Identification and in vitro validation of diagnostic and prognostic biomarkers for lung squamous cell carcinoma

Abstract

Background:The aim of the present study was to find diagnostic and prognostic biomarkers for lung squamous cell carcinoma (LUSC) and to validate key biomarkers in vitro.Methods: RNA sequencing was used to identify differentially expressed mRNAs (DEmRNAs) and differentially expressed long non-coding RNAs (DElncRNAs) in LUSC tissues. RNA sequencing results were validated using a published dataset. Diagnostic and prognostic values of candidate genes were evaluated by receiver-operating characteristic (ROC) curve analysis and survival analysis, respectively. To determine the effect of MIR205HG in LUSC, MIR205HG expression was knocked down in NCI-H520 cells. 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay and Transwell assay were used to respectively detect the effect of MIR205HG on cell proliferation and migration. Results: In total, 1,946 DEmRNAs and 428 DElncRNAs were identified in LUSC compared with normal tissues. A total of 851 DElncRNA-DEmRNA co-expression pairs were obtained. With the exception of NEAT1, MCM2, SERPINB5, ITGB8, CASC19, and MIR205HG were upregulated in LUSC. ROC curve analysis indicated that MCM2, SERPINB5, ITGB8, CASC19, and MIR205HG could predict LUSC. Survival analysis suggested that SERPINB5, NEAT1, and MIR205HG had potential prognostic value for LUSC.MIR205HG knockdown inhibited cell proliferation and migration, and significantly reduced the expression of ITGB8. Conclusions:The findings of the present study could help determine the pathogenesis of LUSC and provide new and accurate therapeutic targets for its treatment.

Data‐independent acquisition and quantification of extracellular matrix from human lung in chronic inflammation‐associated carcinomas

“…SERPINB5 has a tumor suppressive activity [ 74 ]. In LSCC, this protein may be associated with cancer development by regulating the p53 signaling pathway [ 75 ]. SERPINH1 is an endoplasmic reticulum protein with chaperone activity which ensures proper folding and ultimately conformation of type I procollagen trimer [ 76 ].…”

Six-Gene Signature for Differential Diagnosis and Therapeutic Decisions in Non-Small-Cell Lung Cancer—A Validation Study

“…However, this choice may have inadvertently limited our insights into the broader cancer biology, as it omitted potential comparisons with healthy tissues. Additionally, the gene MIR205HG, a pivotal focus in our study, exhibits variations across various cancer types and even within lung squamous cell carcinoma [30,31]. This variability poses challenges for its clinical application as a diagnostic marker.…”

“…Its expression has been reported to vary across different cancer types, being downregulated in esophageal cancer [26], upregulated in hepatoblastoma [27] and cervical cancer [28], and positively correlated with head and neck squamous cell carcinoma, particularly in cases with TP53 mutations [29]. However, in the context of SCC, the existing literature presents conflicting results, some suggesting downregulation while others indicating upregulation in comparison to healthy tissue [30,31].…”

“…In head and neck squamous cell carcinoma, MIR205HG inactivates miR-590-3p, leading to increased expression of cyclin B, cdk1, and YAP [29]. In lung squamous cell carcinoma, it promotes proliferation, migration, and tumor progression by modulating the expression of Bcl-2 and Bax [32], enhancing ITGB8 expression [30], and binding to miR-299-3p, resulting in elevated MAP3K2 expression [31].…”