Identification and in silico characterization of structural and functional impacts of genetic variants in milk protein genes in the Zebu breeds Guzerat and Gyr

Matosinho, Carolina Guimarães Ramos; Rosse, Izinara Cruz; Fonseca, Pablo A S; Oliveira, Francislon Silva de; Santos, Fausto Gonçalves Dos; Araújo, Flávio Marcos Gomes; Salim, Anna Christina de Matos; Lopes, Beatriz Cordenonsi; Arbex, Wagner; Machado, M. A.; Peixoto, Maria Gabriela Campolina Diniz; Verneque, Rui da Silva; Martins, Marta Fonseca; Silva, Marcos Vinícius Gualberto Barbosa da; Oliveira, Guilherme; Pires, Douglas E V; Carvalho, Maria Raquel Santos

doi:10.1007/s11250-021-02970-2

Cited by 3 publications

(1 citation statement)

References 103 publications

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“…There are polymorphism and insertion-deletion sites in the promoter region of the CSN1S1 gene, which affect the protein content in mare milk [28]. Although the promoter sequence of the CSN1S1 gene has been well analyzed in silico, the research on its transcriptional regulation mechanism remains scarce [29]. In this study, we focus on the regulation mechanism of CSN1S1 promoter activity in goat mammary epithelial cells, which contributes to the improvement of milk quality for human consumption [30].…”

Section: Discussionmentioning

confidence: 99%

Mutation of Signal Transducer and Activator of Transcription 5 (STAT5) Binding Sites Decreases Milk Allergen αS1-Casein Content in Goat Mammary Epithelial Cells

Song

Luo

Huang

et al. 2022

Foods

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αS1-Casein (encoded by the CSN1S1 gene) is associated with food allergy more than other milk protein components. Milk allergy caused by αS1-casein is derived from cow milk, goat milk and other ruminant milk. However, little is known about the transcription regulation of αS1-casein synthesis in dairy goats. This study aimed to investigate the regulatory roles of signal transducer and activator of transcription 5 (STAT5) on αS1-casein in goat mammary epithelial cells (GMEC). Deletion analysis showed that the core promoter region of CSN1S1 was located at −110 to −18 bp upstream of transcription start site, which contained two putative STAT5 binding sites (gamma-interferon activation site, GAS). Overexpression of STAT5a gene upregulated the mRNA level and the promoter activity of the CSN1S1 gene, and STAT5 inhibitor decreased phosphorylated STAT5 in the nucleus and CSN1S1 transcription activity. Further, GAS site-directed mutagenesis and chromatin immunoprecipitation (ChIP) assays revealed that GAS1 and GAS2 sites in the CSN1S1 promoter core region were binding sites of STAT5. Taken together, STAT5 directly regulates CSN1S1 transcription by GAS1 and GAS2 sites in GMEC, and the mutation of STAT5 binding sites could downregulate CSN1S1 expression and decrease αS1-casein synthesis, which provide the novel strategy for reducing the allergic potential of goat milk and improving milk quality in ruminants.

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Section: Discussionmentioning

confidence: 99%

Mutation of Signal Transducer and Activator of Transcription 5 (STAT5) Binding Sites Decreases Milk Allergen αS1-Casein Content in Goat Mammary Epithelial Cells

Song

Luo

Huang

et al. 2022

Foods

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Kappa and beta-casein alleles in dairy zebu cattle

Pessoa

Silva

Borba

et al. 2022

Animal Biotechnology

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The CABANA model 2017–2022: research and training synergy to facilitate bioinformatics applications in Latin America

Campos-Sánchez,

Willis,

Gopalasingam

et al. 2024

Front. Educ.

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The CABANA project (Capacity Building for Bioinformatics in Latin America) was funded by the UK’s Global Challenges Research Fund in 2017 with the aim to strengthen the bioinformatics capacity and extend its applications in Latin America focused on three challenge areas – communicable diseases, sustainable food production and protection of biodiversity. For 5 years, the project executed activities including data analysis workshops, train-the-trainer workshops, secondments, eLearning development, knowledge exchange meetings, and research projects in 10 countries. The project was successful in accomplishing all its goals with a major impact on the region. It became a model by which the research needs determined the training that was delivered. Multiple publications and over 800 trainees are part of the legacy of the project.

show abstract

Identification and in silico characterization of structural and functional impacts of genetic variants in milk protein genes in the Zebu breeds Guzerat and Gyr

Cited by 3 publications

References 103 publications

Mutation of Signal Transducer and Activator of Transcription 5 (STAT5) Binding Sites Decreases Milk Allergen αS1-Casein Content in Goat Mammary Epithelial Cells

Mutation of Signal Transducer and Activator of Transcription 5 (STAT5) Binding Sites Decreases Milk Allergen αS1-Casein Content in Goat Mammary Epithelial Cells

Kappa and beta-casein alleles in dairy zebu cattle

The CABANA model 2017–2022: research and training synergy to facilitate bioinformatics applications in Latin America

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