Identification and immunoregulatory role of cathepsin A in the red swamp crayfish, Procambarus clarkii

Huang, Long; Dai, Li-Shang; Wu, Benli; Zhang, Ye; Chen, Jing; He, Junna

doi:10.1016/j.ijbiomac.2020.03.051

Cited by 9 publications

(3 citation statements)

References 38 publications

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“…Altogether, these results allowed us to infer that Pc-cathepsin D-like is involved in the regulation of immune-pathway associated genes. The involvement of cathepsins in the modulation of immune genes is not entirely a new phenomenon, and it has also been reported in many other studies (Liu et al, 2020;Huang et al, 2020). However, further research is required to elucidate molecular mechanisms by which cathepsins regulate immune genes.…”

Section: Clarkiimentioning

confidence: 76%

“…We investigated the spatial expression patterns of Pc-cathepsin D-like gene of P. clarkii and found it to be very similar to those of cathepsins of other crustaceans (Apitanyasai et al, 2016;Sun et al, 2018;Liu et al, 2020;Huang et al, 2020). In healthy and non-induced P. clarkii, Pc-cathepsin D-like mRNA was expressed in all the tested tissues, including muscle, heart, stomach, hepatopancreas, gut, hemocyte, and gill.…”

Section: Discussionmentioning

confidence: 93%

“…Many authors reported the upregulation of cathepsins in tissues/organ of aquatic invertebrates infected with microbial pathogens. For example, cathepsin A and C gene expression were upregulated in the hepatopancreas of crayfish infected with bacterial and viral pathogens (Oidtmann et al, 2004;Liu et al, 2020;Huang et al, 2020). In both cases, the crayfish exposed to pathogens for different time periods to evaluate the change in the expression of cathepsins.…”

Section: R a F Tmentioning

confidence: 99%

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Molecular characterization and functional analysis of the lysosomal cathepsin D-like gene in red swamp crayfish, Procambarus clarkii

Huang

et al. 2021

Genome

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Aspartic proteinases are one of the four families of proteinase enzymes that are widely present in living organisms. They are involved in various physiological events, such as protein degradation, development, and host defense. However, the characterization and functional roles of aspartic proteinases remain to be elucidated in crustaceans. Here, we characterized a fragment of cathepsin D-like cDNA from red swamp crayfish, Procambarus clarkii (Pc-cathepsin D-like). The open reading frame of the Pc-cathepsin D-like gene contained 1152 bp, encoding a protein of 383 amino acid residues. We also evaluated the immunological role of the Pc-cathepsin D-like gene in vivo. Spatial distribution analysis revealed that the Pc-cathepsin D-like mRNA was high in the hepatopancreas, followed by the gut, gills, and hemocytes of P. clarkii. The expression levels of the Pc-cathepsin D-like gene increased following challenge with viral (polyinosinic: polycytidylic acid) and bacterial (lipopolysaccharides, peptidoglycan) PAMPs compared with PBS injection. The suppression of the Pc-cathepsin D-like gene by RNA interference significantly increased the expression of immune-associated genes. These results showed that the Pc-cathepsin D-like gene has an essential biological role in innate immune responses because it regulates the expression of immune-associated genes.

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Section: Clarkiimentioning

confidence: 76%