Identification and Immunological Characterization of Somatic Proteins from Adults of Toxocara cati by Proteomics Technique

Abstract

Background: Toxocara cati is considered as one of the main etiological agents of toxocariasis with global and regional importance. As there is no information on proteomics of T. cati, herein, we reported the results obtained by proteomic analysis of somatic proteins extract, using a mass spectrometry (LC–MS/MS) approach.
 Methods: Somatic extract fractions were separated by two-dimensional SDSPAGE and were electro blotted on to PVDF membranes for immunoblot analysis, then collected the immunogenic spots which response of antibodies of the paratenic hosts (mice) to the antigens ( Mashhad, 2017), and analyzed by LC–MS/MS. The LC-MS/MS data were analyzed by Mascot database, Taxonomy Toxocara, and common contaminants, in Omics Center, Biotechnology Medical University of Graz (Austria, 2018).
 Result: The protein spots were isolated between 15–140 kDa ranges using 3–10 non-linear IPG strips and Brilliant Blue Coomassie. Ten proteins were characterized as immunogenic proteins, seven of them were identified and three of them were unknown proteins.
 Conclusion: This study provided additional information about the somatic antigens of T. cati, which can lead to the development of new strategies for novel immuno-modulators, drug targets, subunit vaccines and immunodiagnostic kits for toxocariasis.

Comprehensive control of toxocariasis in communities

“…Metabolomics studies were also carried out, where the nonprotein components of the parasite extract were characterized for the three metabolic pathways: fatty acid, amino acid, and carbohydrate metabolism [71]. Soleymani et al [72] through proteomic analyses identified a variety of proteins from the soluble extract of T. c a t i adults, which could have a role in the host-parasite interaction, as well as 10 somatic proteins of this parasite with the capacity to generate an immune response by the host.…”

Evaluation of Somatic Antigens of Adult Toxocara helminthes for Detection of Human Toxocariasis

“…In addition, they could be more reliable for assessment in larger sample size [9,14,15]. The immune reactive bands of T. canis crude antigen were revealed with dog serum and the reactivity properties of somatic proteins of T. cati adult worms were identified using techniques such as proteomics [16][17][18].…”