Identification and glass biodeterioration of Chaetomium globosum TTHF1-3 isolated from optical instrument at Thai Hoa, Nghe An province

Abstract

The colonization and growth of harmful fungi on the glass surface have caused irreversible damage to optical quality. Harmful grades observed on the optical instruments depend on the biological characteristics of fungi that vary from species to species. The present study focused on isolation, identification, and evaluation of glass biodeterioration properties such as organic acid and exopolysaccharide production of fungal strain Chaetomium globosum TTHF1-3 isolated from lens of optical instrument collected at Thai Hoa, Nghe An province. Under microscopic observation, the fungal strain TTHF1-3 cells showed brown or dark brown color perithecia and ascospores. Based on ITS sequence analyses, the strain TTHF1-3 was found to share 100% sequence identity with that of C. globosum species deposited on GenBank (NCBI). A in situ biodeterioration test exhibited the hyphal surface coverage of strain TTHF1-3 reaching 29.77±1.15%, which corresponded to harmful grade 2 based on the ISO 9022-11:2015 criteria. When incubated on MT4 medium containing glucose and mineral elements, the pH values of C. globosum TTHF1-3 culture were significantly decreased from 6.5 to 3.12±0.12, which was in contrast to MT1 medium. In addition, fungal strain TTHF1-3 was able to produce 8.2±0.3 g/L exopolysaccharides. The findings in the present study confirmed that C. globosum TTHF1-3 was harmful fungus responsible for glass biodeterioration.