Identification and genetic diversity of Spodoptera frugiperda in Lampung Province, Indonesia

Abstract

Abstract. Lestari P, Budiarti A, Fitriana Y, Susilo FX, Swibawa IG, Sudarsono H, Suharjo R, Hariri AM, Purnomo, Nuryasin, Solikhin, Wibowo L, Jumari, Hartaman M. 2020. Identification and genetic diversity of Spodoptera frugiperda in Lampung Province, Indonesia. Biodiversitas 21: 1670-1677. Spodoptera frugiperda is one of the most recent invasive and destructive insect pest in Indonesia. Recently, it has been reported that this pest was found in some cornfield areas in Sumatera, including Lampung. This research was performed to confirm the presence of S. frugiperda in Lampung Province by collecting and identifying larvae of Spodoptera found in the field as well as investigation on the genetic diversity of the established populations and to observe the damage caused by this pest on cornfields in the Lampung Province. The observation was conducted from February-April 2019 at four locations (districts) representing corn-producing areas in Lampung, namely Lampung Selatan, Lampung Timur, Pesawaran and Pringsewu, each location comprising five plots. The plot is a cornfield with plants aged 14-40 days after planting. Twenty plants were randomly chosen in every plot as plant samples to collect the Spodoptera larvae and to calculate the absolute plant damage caused by the larvae. The absolute plant damage was analyzed by dividing the attacked plants with total plants observed and multiply by 100%. Identification of the Spodoptera larvae was performed based on morphological characters and molecular techniques using sequence analysis of Cytochrome c Oxidase subunit I (COI) gene. The result confirmed that the larvae found in the cornfield in Lampung were S. frugiperda. There was no nucleotides variation in the sequence of COI gene among S. frugiperda found in Lampung Province (Lampung Selatan, Lampung Timur, Pesawaran and Pringsewu) as well as S. frugiperda that was found in corn from foreign countries. The absolute plant damage caused by this pest in the four districts of Lampung was in the range of 26.50-70%.

The endophytic fungi from South Sumatra (Indonesia) and their pathogenecity against the new invasive fall armyworm, Spodoptera frugiperda

“…Maize in Indonesia is currently facing a big problem in invasion and outbreaks of newcomer insect pests, namely the fall armyworm (Spodoptera frugiperda) (Lepidoptera: Noctuidae). S. frugiperda comes from South America (Nagoshi et al 2017;Otim et al 2018) and entered Indonesia for the first time on March 26, 2019 in West Sumatra, then in June 2019 it was found in Banten and West Java (Sartiami et al 2020) and now it has spread rapidly to various provinces in Indonesia, such as South Sumatra (Herlinda et al 2020b;Hutasoit et al 2020), Lampung (Lestari et al 2020), and Bengkulu (Ginting et al 2020). The fall armyworm has caused maize yield losses in Africa of 250-630 million US dollars per year (Bateman et al 2018).…”

Exploring entomopathogenic fungi from South Sumatra (Indonesia) soil and their pathogenicity against a new invasive maize pest, Spodoptera frugiperda

“…The pest began to enter Asia in 2018 and was first discovered in India (Ganiger et al 2018). In April 2019, the FAW reportedly began entering Indonesia and was first discovered in West Sumatra, after which it spread throughout Sumatra, including Lampung (Lestari et al 2020) and Palembang. Then, the pest crossed to Java and Kalimantan (IPPC 2019).…”

Spatial distribution pattern, bionomic, and demographic parameters of a new invasive species of armyworm Spodoptera frugiperda (Lepidoptera; Noctuidae) in maize of South Sumatra, Indonesia

“…Nonci et al (2019) reported that this pest has entered and attacked the maize plantation in Indonesia, precisely in Koto Baru, Luhak Nan Duo West Pasaman, West Sumatra. This pest quickly spread to several locations in Indonesia (Maharani et al 2019;Lestari et al 2020). Based on the survey results conducted to farmers, the damage and loss of yield caused by S. frugiperda in maize plantations in Africa were reported to be very serious.…”