Identification and Genetic Analysis of Pseudomonas fluorescence in Milk and Milk Products in Mosul City

Abstract

HE CURRENT study is aimed to study the prevalence of P. flouresence in milk and its products of cows and ewes at Mosul city. Fifty samples from bovine and ovine dairy products were collected from outdoor public markets in Mosul city. The samples included 20 samples from each of raw milk and cheese from both of cows and ewes and 10 yoghurt samples. P.flouresence were identified using 16SrRNA gene by conventional polymerase chain reaction technique. In order to identify the similarities and differences between the Iraqi strains and the currently recognized worldwide strains, for the sequenced 16srRNA was examined using BLAST analysis. Results revealed the presence of P.flouresence in ( 70%) and (60%) of cows' raw milk and cheese respectively whereas (20%) and (10%) of ewes' raw milk and cheese were positive for P.flouresence . All yoghurt samples were negative for the recovery of P.flouresence in cows and ewes. Three isolates of P.flouresence from cows' raw milk were recorded in Genebank with accession number LC745963, LC745964 and LC745965 respectively. According to phylogenetic tree the local strains were similar to strains recorded in United Kingdom (FM872377.1).