Identification and functional characterization of ten AP2/ERF genes in potato

Bouaziz, Donia; Charfeddine, Mariam; Jbir, Rania; Saidi, Mohammed Najib; Pirrello, Julien; Charfeddine, Safa; Bouzayen, Mondher; Gargouri-Bouzid, Radhia

doi:10.1007/s11240-015-0823-2

Cited by 29 publications

(9 citation statements)

References 108 publications

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“…Similar results were also observed in JA‐ and SA‐induced genes, such as IbERF5 and IbERF7 which were upregulated by JA and SA treatments, and were increased by different abiotic stresses. The data are similar to other reports, such as potato StERF3 and StERF6 genes were induced by salt, ABA, JA, and SA treatments (Bouaziz et al., 2015). These findings suggested that multiple IbERF s might participate in JA or SA pathways, and might be involved in the biotic response of sweetpotato.…”

Section: Discussionsupporting

confidence: 92%

“…Jasmonic acid and SA play crucial roles in the defense response of plants to pathogens and abiotic stress (Bari & Jones, 2009). Multiple studies in potato (Bouaziz et al., 2015), tomato (Sharma et al., 2010), and rice (Sharoni et al., 2011) have shown that some AP2/ERFs could regulate stress response by both ABA‐dependent and ABA‐independent pathways in plants. The expression of seven IbERF s was obviously upregulated by ABA.…”

Section: Discussionmentioning

confidence: 99%

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Identification, expression analysis, and functional characterization of salt stress‐responsive genes of AP2/ERF transcription factors in sweetpotato

et al. 2020

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The AP2/ERF transcription factors (TFs) regulate various processes of plant growth, development, and response to environmental stimuli. Although they have been extensively documented in many plants, little is known about stress responsive APETALA2/ethylene response factor (AP2/ERF) genes in sweetpotato (Ipomoea batatas L.). In this study, 20 putative AP2/ERF genes, named IbERF1, IbERF2, and IbERF5–IbERF23 were identified based on the salt‐treated RNA‐seq data from two sweetpotato cultivars with different salt tolerance. Phylogenetic analysis and sequence characterization identified that all the screened IbERF proteins contained a conserved AP2 domain, which belonged to dehydration‐responsive element binding proteins (DREB; 7 members) or ERF (13 members) subfamily. The systematic expression analysis of 12 mainly salt‐induced IbERFs by quantitative real‐time polymerase chain reaction (qRT‐PCR) revealed that most IbERFs were remarkably increased by multiple abiotic stresses, such as NaCl, dehydration, and cold stress. Furthermore, varying degrees of upregulation were also detected in the expression of most IbERFs when treated with hormone abscisic acid, jasmonic acid, and salicylic acid. The expression profiles of IbERFs corroborate their involvement in diverse biological processes. In addition, the IbERF5, which was exclusively upregulated by the treatments, was identified as a nuclear protein without transcriptional activation activity. Collectively, the data of stress responsive IbERFs will provide valuable knowledge for further functional studies of IbERFs in sweetpotato stress tolerance.

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Section: Discussionsupporting

confidence: 92%

Section: Discussionmentioning

confidence: 99%

Identification, expression analysis, and functional characterization of salt stress‐responsive genes of AP2/ERF transcription factors in sweetpotato

et al. 2020

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“…The drought‐responsive element binding (DREB) factors comprise a subfamily of the AP2/ERF family of TFs containing single AP2 DNA‐binding domains, which recognize six nucleotides (A/G)CCGAC of the dehydration‐responsive element/C‐repeat (DRE/CRT). These cis ‐elements are located on promoter regions of target stress‐responsive genes and play an important role in regulation of stress‐inducible transcription (Agarwal et al ., ; Bouaziz et al ., ; Hrmova and Lopato, ; Sakuma et al ., ).…”

Section: Introductionmentioning

confidence: 98%

DREB/CBF expression in wheat and barley using the stress‐inducible promoters of HD‐Zip I genes: impact on plant development, stress tolerance and yield

Yang

Al-Baidhani

Harris

et al. 2019

Plant Biotechnology Journal

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Summary Networks of transcription factors regulate diverse physiological processes in plants to ensure that plants respond to abiotic stresses rapidly and efficiently. In this study, expression of two DREB/CBF genes, TaDREB3 and TaCBF5L, was modulated in transgenic wheat and barley, by using stress‐responsive promoters HDZI‐3 and HDZI‐4. The promoters were derived from the durum wheat genes encoding the γ‐clade TFs of the HD‐Zip class I subfamily. The activities of tested promoters were induced by drought and cold in leaves of both transgenic species. Differences in sensitivity of promoters to drought strength were dependent on drought tolerance levels of cultivars used for generation of transgenic lines. Expression of the DREB/CBF genes under both promoters improved drought and frost tolerance of transgenic barley, and frost tolerance of transgenic wheat seedlings. Expression levels of the putative TaCBF5L downstream genes in leaves of transgenic wheat seedlings were up‐regulated under severe drought, and up‐ or down‐regulated under frost, compared to those of control seedlings. The application of TaCBF5L driven by the HDZI‐4 promoter led to the significant increase of the grain yield of transgenic wheat, compared to that of the control wild‐type plants, when severe drought was applied during flowering; although no yield improvements were observed when plants grew under well‐watered conditions or moderate drought. Our findings suggest that the studied HDZI promoters combined with the DREB/CBF factors could be used in transgenic cereal plants for improvement of abiotic stress tolerance, and the reduction of negative influence of transgenes on plant development and grain yields.

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“…So far, a few salt-responsive genes have been identified in potato, such as zinc finger protein gene (StZFP1) 16 ; ethylene-responsive transcription factors (StERF1, StERF3 and StERF6) 17 ; dehydration-responsive element-binding protein genes (StDREB1, StDREB2, StDREB3 and StDREB4) 17,18 . With the development of high throughput sequencing technology, RNA-seq has been more and more frequently used in plant stress resistance research.…”

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confidence: 99%

Transcriptome analysis uncovers the gene expression profile of salt-stressed potato (Solanum tuberosum L.)

Qin

et al. 2020

Sci Rep

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potato (Solanum tuberosum L.) is an important staple food worldwide. However, its growth has been heavily suppressed by salt stress. The molecular mechanisms of salt tolerance in potato remain unclear. It has been shown that the tetraploid potato Longshu No. 5 is a salt-tolerant genotype. Therefore, in this study we conducted research to identify salt stress response genes in Longshu No. 5 using a NaCl treatment and time-course RNA sequencing. The total number of differentially expressed genes (DEGs) in response to salt stress was 5508. Based on Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis, it was found that DEGs were significantly enriched in the categories of nucleic acid binding, transporter activity, ion or molecule transport, ion binding, kinase activity and oxidative phosphorylation. Particularly, the significant differential expression of encoding ion transport signaling genes suggests that this signaling pathway plays a vital role in salt stress response in potato. Finally, the DEGs in the salt response pathway were verified by Quantitative real-time PCR (qRT-PCR). These results provide valuable information on the salt tolerance of molecular mechanisms in potatoes, and establish a basis for breeding salt-tolerant cultivars. Salt is a major abiotic factor affecting plant growth and secondary metabolism 1. Soil salinization has become a global problem with about 8 × 10 8 hectares of soil worldwide threatened by salinization 2. Salinity interferes with plant growth as it leads to physiological drought and ion toxicity 3. In addition, other secondary stresses, such as oxidative damage, can occur in plants subjected to high NaCl concentrations 1. With the increase of salinization, it is a tough challenge to increase grain output and achieve food security. Potato (Solanum tuberosum L.) is an extremely important food staple worldwide due to its versatility and nutritional value. However, potato is quite sensitive to salt stress, which is one of the most important factors limiting its cultivation 4 and which can lead to serious declines in yield 5,6. Therefore, there is a great need to improve the salt tolerance of potato and breed salt-tolerant varieties. What's more, illuminating the molecular mechanisms underlying salt tolerance and identifying the related genes of tolerant plants may contribute to further understanding the functions of these unique genes. Previous studies have revealed mechanisms underlying salt stress tolerance in plants. Plant membrane receptors sense extracellular salt stress stimuli, and then these stimuli signals are translated into intracellular signals through the generation of second messengers such as calcium, reactive oxygen species (ROS) and inositol phosphates. These second messengers then activate transcription factors (TFs) or protein kinases (PKs), inducing specific genes to be differentially expressed 7. These signal cascades result in the expression of multiple stress-responsive genes, the products of which can directly or indirectly confer s...

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Identification and functional characterization of ten AP2/ERF genes in potato

Cited by 29 publications

References 108 publications

Identification, expression analysis, and functional characterization of salt stress‐responsive genes of AP2/ERF transcription factors in sweetpotato

Identification, expression analysis, and functional characterization of salt stress‐responsive genes of AP2/ERF transcription factors in sweetpotato

DREB/CBF expression in wheat and barley using the stress‐inducible promoters of HD‐Zip I genes: impact on plant development, stress tolerance and yield

Transcriptome analysis uncovers the gene expression profile of salt-stressed potato (Solanum tuberosum L.)

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