Identification and functional characterization of the ZmCOPT copper transporter family in maize

Wang, Hongling; Du, Hanmei; Li, Hongyou; Huang, Ying; Ding, Jianzhou; Lee, Chan; Wang, Ning; Long, Hai; Zhang, Suzhi

doi:10.1371/journal.pone.0199081

Cited by 16 publications

(12 citation statements)

References 43 publications

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“…The genome wide identification, expression analysis and functional characterization of NRT1 family transporters in sorghum may help to improve the NUE of sorghum and other closely related cereals, particularly small millets. Plant NRTs are encoded by 4 gene families, NRT1/peptide transporter family (NPF), NRT2, chloride channel (CLC) and slow anion channel-associated 2 (SLAC2)/ SLAC homologues (SLAH) (Fan et al 2017;Wang et al 2018a). To date only one work related to sorghum's NRT family transporter has been published (Huang et al 1999).…”

Section: Identification Of Qtl and Transporters For Improving N Use-efficiency (Nue)mentioning

confidence: 99%

Improving abiotic stress tolerance in sorghum: focus on the nutrient transporters and marker-assisted breeding

Maharajan¹,

Krishna²,

Kiriyanthan

et al. 2021

Planta

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Main conclusion Identification of molecular markers and characterization of nutrient transporters could help to improve the tolerance under abiotic and low nutrient stresses in sorghum ensuring higher yield to conserve food securityAbstract Sorghum is an important cereal crop delivering food and energy security in the semi-arid tropics of the world. Adverse climatic conditions induced by global warming and low input agriculture system in developing countries demand for the improvement of sorghum to tolerate various abiotic stresses. In this review, we discuss the application of marker-assisted breeding and nutrient transporter characterization studies targeted towards improving the tolerance of sorghum under drought, salinity, cold, low phosphate and nitrogen stresses. Family members of some nutrient transporters such as nitrate transporter (NRT), phosphate transporter (PHT) and sulphate transporter (SULTR) were identified and characterized for improving the low nutrient stress tolerance in sorghum. Several quantitative trait loci (QTL) were identified for drought, salinity and cold stresses with an intention to enhance the tolerance of sorghum under these stresses. A very few QTL and nutrient transporters have been identified and validated under low nitrogen and phosphorus stresses compared to those under drought, salinity and cold stresses. Marker-assisted breeding and nutrient transporter characterization have not yet been attempted in sorghum under other macro-and micro-nutrient stresses. We hope this review will raise awareness among plant breeders, scientists and biotechnologists about the importance of sorghum and need to conduct the studies on marker-assisted breeding and nutrient transporter under low nutrient stresses to improve the sorghum production.

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Section: Identification Of Qtl and Transporters For Improving N Use-efficiency (Nue)mentioning

confidence: 99%

Improving abiotic stress tolerance in sorghum: focus on the nutrient transporters and marker-assisted breeding

Maharajan¹,

Krishna²,

Kiriyanthan

et al. 2021

Planta

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“…To evaluate Cu transporters in Lotus spp., L. japonicus was chosen for two main reasons: a) It is a model species whose genome has been completely sequenced [54]; and b) in response to partial submergence, its Cu levels are markedly reduced, leading to Cu deficiency (Figure 2A). Previously, the relevance of the COPT family of transporters has been evaluated in other species of agronomic interest, such as rice, maize, or vine [35,36,37,55]. In legumes, COPT members have been predicted by in silico analysis for Glycine max , Phaseolus vulgaris , and Medicago truncatula , among others.…”

Section: Discussionmentioning

confidence: 99%

“…Some of these proteins have been characterized in the species of agronomical interest. Thus, seven genes encoding Cu transporters have been described in rice ( Oryza Sativa ) [35]; eight members in grapevine ( Vitis vinifera ) [36]; three proteins in maize [37]; and seven in Populus trichocarpa [37]. Recently, the Copper Transporter 1 (MtCOPT1) protein from the legume Medicago truncatula , has been functionally characterized as a Cu transporter from the apoplast into nodule cells [13].…”

Section: Introductionmentioning

confidence: 99%

Characterization of the Copper Transporters from Lotus spp. and Their Involvement under Flooding Conditions

Escaray

Antonelli

Copello

et al. 2019

IJMS

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Forage legumes are an important livestock nutritional resource, which includes essential metals, such as copper. Particularly, the high prevalence of hypocuprosis causes important economic losses to Argentinian cattle agrosystems. Copper deficiency in cattle is partially due to its low content in forage produced by natural grassland, and is exacerbated by flooding conditions. Previous results indicated that incorporation of Lotus spp. into natural grassland increases forage nutritional quality, including higher copper levels. However, the biological processes and molecular mechanisms involved in copper uptake by Lotus spp. remain poorly understood. Here, we identify four genes that encode putative members of the Lotus copper transporter family, denoted COPT in higher plants. A heterologous functional complementation assay of the Saccharomyces cerevisiae ctr1∆ctr3∆ strain, which lacks the corresponding yeast copper transporters, with the putative Lotus COPT proteins shows a partial rescue of the yeast phenotypes in restrictive media. Under partial submergence conditions, the copper content of L. japonicus plants decreases and the expression of two Lotus COPT genes is induced. These results strongly suggest that the Lotus COPT proteins identified in this work function in copper uptake. In addition, the fact that environmental conditions affect the expression of certain COPT genes supports their involvement in adaptive mechanisms and envisages putative biotechnological strategies to improve cattle copper nutrition.

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“…Plants mainly respond to high Cu homeostasis through various plant Cu transporters (COPT), heavy metal transporting ATPase (HMA), zinc/iron-regulated transporters (ZIP), and yellow stripe-like protein (YSL), among which COPT has the most abundant functions and is widely involved in Cu uptake, distribution, and recirculation in cells of Arabidopsis (Arabidopsis thaliana), rice ( O. sativa ), green algae ( Chlamydomonas reinhardtii ), and corn ( Z. mays ) plants . Meanwhile, in terms of the effects of different forms of Cu sources on plant growth and toxicity, the current research focus is mainly on a comparison with Cu-based NPs and inorganic Cu, while other forms of Cu sources, such as Cu–glycine and Cu–proteinate, have been less reported.…”

Section: Copper and Plantsmentioning

confidence: 99%

Latent Benefits and Toxicity Risks Transmission Chain of High Dietary Copper along the Livestock–Environment–Plant–Human Health Axis and Microbial Homeostasis: A Review

Zhen

Chen

et al. 2022

J. Agric. Food Chem.

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The extensive use of high-concentration copper (Cu) in feed additives, fertilizers, pesticides, and nanoparticles (NPs) inevitably causes significant pollution in the ecological environment. This type of chain pollution begins with animal husbandry: first, Cu accumulation in animals poisons them; second, high Cu enters the soil and water sources with the feces and urine to cause toxicity, which may further lead to crop and plant pollution; third, this process ultimately endangers human health through consumption of livestock products, aquatic foods, plants, and even drinking water. High Cu potentially alters the antibiotic resistance of soil and water sources and further aggravates human disease risks. Thus, it is necessary to formulate reasonable Cu emission regulations because the benefits of Cu for livestock and plants cannot be ignored. The present review evaluates the potential hazards and benefits of high Cu in livestock, the environment, the plant industry, and human health. We also discuss aspects related to bacterial and fungal resistance and homeostasis and perspectives on the application of Cu−NPs and microbial high-Cu removal technology to reduce the spread of toxicity risks to humans.

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Identification and functional characterization of the ZmCOPT copper transporter family in maize

Cited by 16 publications

References 43 publications

Improving abiotic stress tolerance in sorghum: focus on the nutrient transporters and marker-assisted breeding

Improving abiotic stress tolerance in sorghum: focus on the nutrient transporters and marker-assisted breeding

Characterization of the Copper Transporters from Lotus spp. and Their Involvement under Flooding Conditions

Latent Benefits and Toxicity Risks Transmission Chain of High Dietary Copper along the Livestock–Environment–Plant–Human Health Axis and Microbial Homeostasis: A Review

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