Identification and functional characterization of antimicrobial peptide from the marine crab Dromia dehaani

Anbuchezian, R.; Ravichandran, S.; Rajan, Durairaj Karthick; Tilivi, Supriya; Devi, Shikha

doi:10.1016/j.micpath.2018.08.056

Cited by 22 publications

(14 citation statements)

References 30 publications

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“…Samples of about (∼5 mg) was dissolved in D 2 O (99.96 atom %). One dimensional 1 H NMR was performed on at 400MHZ on a Bruker model 500 NMR type (Anbuchezian et al 2018).…”

Section: Nuclear Magnetic Resonancementioning

confidence: 99%

Identification and characterization of peptides from marine crustacean crabs and their bioactive properties; An approach to develop novel drugs for Alzheimer’s disease

Bakthavachalam

Sivakumar

2023

Preprint

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Peptide-based drugs have become the most promising drug therapy in the pharmaceutical industry. It accommodates a major part in treating diseases like neurodegenerative disorders, type 2 diabetes, blood pressure, etc. This study aims to isolate and characterize the marine peptides from the crustacean crab species named Scylla Serrata. The hemolymph was collected and subjected to thin-layer chromatography (TLC) and Fourier Transform Infrared spectroscopy for identifying amine groups. Further the peptide groups in the sample was confirmed by nuclear magnetic resonance analysis. Ultra-performance liquid chromatography (UPLC) for the crude was performed to identify the intensity of the peaks. The sample was further purified with Sephadex G-25 to elute the small molecular peptides. Antioxidant activity (DPPH) for the crude sample showed 55% of activity at 100µg/ml concentration and the fractions showed significant activity. The eluted fractions from the column chromatography were then subjected to SDS PAGE to identify the molecular weight of the peptides in which the peptide bands ranged between 75–100 kDa. Further, the best compounds must be developed as potential drugs for Alzheimer’s disease with an In-silico approach.

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“…Samples of about (∼5 mg) was dissolved in D 2 O (99.96 atom %). One dimensional 1 H NMR was performed on at 400MHZ on a Bruker model 500 NMR type (Anbuchezian et al 2018).…”

Section: Nuclear Magnetic Resonancementioning

confidence: 99%

Identification and characterization of peptides from marine crustacean crabs and their bioactive properties; An approach to develop novel drugs for Alzheimer’s disease

Bakthavachalam

Sivakumar

2023

Preprint

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“…Table 1. Summary of the techniques used for the preparation and purification of marine bioactive peptides commercially [72][73][74][75][76][77][78][79][80][81][82][83][84][85]. Choline-oxalic acid based on eutectic solvent * HPLC (High performance liquid chromatography), reverse-phase-high-performance liquid chromatography, RP-HPLC, mass spectrometry (MS), liquid chromatography followed by tandem mass spectrometric detection (LC-MS/MS), electrospray ionization (ESI), matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF), high-performance liquid chromatography with evaporative light scattering detection (HPLC-ELSD), ultra-high-performance liquid chromatography-tandem mass spectrometry (UHPLC-MS/MS), and liquid chromatography-tandem mass spectrometry (RRLC-MS).…”

Section: Techniques Used For the Commercial Preparation And Purificat...mentioning

confidence: 99%

Therapeutic Potential of Marine Bioactive Peptides against Human Immunodeficiency Virus: Recent Evidence, Challenges, and Future Trends

et al. 2022

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Acquired immunodeficiency syndrome (AIDS) is a chronic and potentially fatal ailment caused by the human immunodeficiency virus (HIV) and remains a major health problem worldwide. In recent years, the research focus has shifted to a greater emphasis on complementing treatment regimens involving conventional antiretroviral (ARV) drug therapies with novel lead structures isolated from various marine organisms that have the potential to be utilized as therapeutics for the management of HIV-AIDS. The present review summarizes the recent developments regarding bioactive peptides sourced from various marine organisms. This includes a discussion encompassing the potential of these novel marine bioactive peptides with regard to antiretroviral activities against HIV, preparation, purification, and processing techniques, in addition to insight into the future trends with an emphasis on the potential of exploration and evaluation of novel peptides to be developed into effective antiretroviral drugs.

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“…Previous research has demonstrated the presence of functional molecules, such as antimicrobial peptides (AMPs), which exhibit antimicrobial activity in marine crustacean decapods, including SC (Beaulieu et al, 2010;El Menif et al, 2018;Haug et al, 2002;Sperstad et al, 2011). AMPs are small cationic peptides that display a broad range of antimicrobial activity against Gram-positive and Gram-negative bacteria (Yue et al, 2010); they can typically be found in the hemocytes of the hemolymph of the marine crabs as an integral component of their immune system (Anbuchezian et al, 2018), and their levels and activity can be related to the stress status of the crab (Coates and Söderhäll, 2020). For some crustacean species, including the spider crab (Hyas araneus) and shore crab (Carcinus maenas), AMPs have displayed stability to heat, specifically when exposed to 85 • C for 15 min (Haug et al, 2002) and 100 • C for 10 min (Relf et al, 1999), respectively.…”

Section: Effect Of Lh Time and Temperature In Scmentioning

confidence: 99%

Live holding of red king crab (Paralithodes camtschaticus) and snow crab (Chionoecetes opilio) — Effect on microbial growth in processed leg meat during refrigerated storage

Lian¹,

Jøstensen²,

Siikavuopio³

et al. 2022

Food Microbiology

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The red king crab (RKC, Paralithodes camtschaticus) and snow crab (SC, Chionoecetes opilio) are valuable decapods that can undergo live holding (LH) in onshore facilities before either live export or processing into two cookedfrozen sections (i.e., clusters). This study investigated the effect of the LH time (up to two months without feeding) and temperature (5|10 • C for RKC; 1|5 • C for SC) on the total viable psychrotrophic count (TVC P ) and Pseudomonas spp. in the leg meat of cooked RKC and SC. The effect of freezing after cooking was also evaluated. The counts were determined during storage at 4 • C after cooking on the clusters undergoing either immediate refrigeration (IR) or 24-month frozen storage before refrigeration (FBR). In the RKC cooked leg meat, the LH temperature significantly affected the TVC P , with LH at 10 • C leading to higher counts, while the Pseudomonas spp. levels were mainly influenced by the freezing, with lower levels in FBR samples compared to their IR counterparts. In the SC cooked leg meat, the LH conditions did not significantly affect the counts, which were instead significantly lowered by the freezing. From an industrial and commercial standpoint, it can be concluded that LH of RKC at high temperature (10 • C) led to a shorter microbial shelf-life of cooked RKC clusters, with the clusters undergoing FBR showing longer microbial shelf-life than their IR counterparts. By contrast, with the mild cooking regime applied, the LH time and temperature of SC had a lower influence on the microbial shelf-life of cooked SC clusters than that given by the choice of the storage type (IR or FBR) after cooking.

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Identification and functional characterization of antimicrobial peptide from the marine crab Dromia dehaani

Cited by 22 publications

References 30 publications

Identification and characterization of peptides from marine crustacean crabs and their bioactive properties; An approach to develop novel drugs for Alzheimer’s disease

Identification and characterization of peptides from marine crustacean crabs and their bioactive properties; An approach to develop novel drugs for Alzheimer’s disease

Therapeutic Potential of Marine Bioactive Peptides against Human Immunodeficiency Virus: Recent Evidence, Challenges, and Future Trends

Live holding of red king crab (Paralithodes camtschaticus) and snow crab (Chionoecetes opilio) — Effect on microbial growth in processed leg meat during refrigerated storage

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