Identification and functional characterization of a c-type lysozyme from Fenneropenaeus penicillatus

Cai, Shuanghu; Zhang, Yilin; Wu, Fan; Wu, Rimin; Yang, Shiping; Li, Ya; Xu, Youhou

doi:10.1016/j.fsi.2019.02.043

Cited by 25 publications

(10 citation statements)

References 32 publications

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“…It catalyzes the hydrolysis of bacterial cell walls and acts as a non-specific innate immunity factor against the invasion of bacterial pathogens (Jollès and Jollès, 1984). Some recent studies found that LYZ also shows antiviral activity against WSSV and IHHNV in shrimp (Mai and Wang, 2010;Cai et al, 2019). The melanization pathway activated by the proPO system is a principal innate immune response in shrimp (Amparyup et al, 2013;Charoensapsri et al, 2014).…”

Section: Discussionmentioning

confidence: 99%

The Molecular Mechanism of Hemocyte Immune Response in Marsupenaeus japonicus Infected With Decapod Iridescent Virus 1

Zhao

Chen

et al. 2021

Front. Microbiol.

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As a new type of shrimp lethal virus, decapod iridescent virus 1 (DIV1) has caused huge economic losses to shrimp farmers in China. Up to now, DIV1 has been detected in a variety of shrimps, but there is no report in Marsupenaeus japonicus. In the current study, we calculated the LC50 to evaluate the toxicity of DIV1 to M. japonicus and determined through nested PCR that M. japonicus can be the host of DIV1. Through enzyme activity study, it was found that DIV1 can inhibit the activities of superoxide dismutase, catalase, lysozyme, and phenoloxidase, which could be a way for DIV1 to achieve immune evasion. In a comprehensive study on the transcriptomic changes of M. japonicus in response to DIV1 infection, a total of 52,287 unigenes were de novo assembled, and 20,342 SSR markers associated with these unigenes were obtained. Through a comparative transcriptomic analysis, 6,900 differentially expressed genes were identified, including 3,882 upregulated genes and 3,018 downregulated genes. The Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analysis showed that some GO terms related to virus invasion, replication, and host antiviral infection were promoted under DIV1 infection, such as carbohydrate binding, chitin binding, chitin metabolic process, and DNA replication initiation, and some KEGG pathways related to immune response were significantly influenced by DIV1 infection, including Toll and IMD signaling pathway, JAK-STAT signaling pathway, IL-17 signaling pathway, C-type lectin receptor signaling pathway, complement and coagulation cascades, antigen processing and presentation, necroptosis, apoptosis, NOD-like receptor signaling pathway, apoptosis—multiple species, and TNF signaling pathway. Further analysis showed that STAT, Dorsal, Relish, heat shock protein 70 (HSP70), C-type lectins, and caspase play an important role in DIV1 infection. This is the first detailed study of DIV1 infection in M. japonicus, which initially reveals the molecular mechanism of DIV1 infection in M. japonicus by using the transcriptome analysis of hemocytes combined with enzyme activity study.

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Section: Discussionmentioning

confidence: 99%

The Molecular Mechanism of Hemocyte Immune Response in Marsupenaeus japonicus Infected With Decapod Iridescent Virus 1

Zhao

Chen

et al. 2021

Front. Microbiol.

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“…ACP is a typical lysosome enzyme involved in killing and digesting pathogens ( 61 ). LZM also has potent non-specific antimicrobial activity ( 62 ). Moreover, as a component of antioxidant defenses, enzymatic antioxidants can protect fish against oxidative damage ( 63 , 64 ).…”

Section: Discussionmentioning

confidence: 99%

Fish Galectin8-Like Exerts Positive Regulation on Immune Response Against Bacterial Infection

et al. 2020

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Galectin-8 is a member of the galectin family that is involved in immune response against pathogens. However, the roles of fish galectin-8 during pathogen infection require comprehensive studies. In this study, a galectin-8 homolog (OnGal8-like, OnGal8-L) was characterized from Nile tilapia (Oreochromis niloticus), and its roles in response to bacterial infection were analyzed. The OnGal8-L contains an open reading frame of 891 bp, encoding a peptide of 296 amino acids with two CRD regions of tandem-repeat galectin and two carbohydrate recognition sites. The OnGal8-L protein shares 46.42% identities with reported Oreochromis niloticus galectin-8 protein. Transcriptional expression analysis revealed that OnGal8-L was constitutively expressed in all examined tissues and was highly expressed in spleen. The transcript levels of OnGal8-L were up-regulated in the spleen, head kidney, and brain, following Streptococcus agalactiae (S. agalactiae) challenge. Further in vitro analysis indicated that the recombinant protein of OnGal8-L (rOnGal8L) could agglutinate erythrocyte, S. agalactiae, and A. hydrophila and bind S. agalactiae, A. hydrophila, and various PAMPs (lipopolysaccharides, lipoteichoic acid, poly I:C, peptidoglycan, galactose, mannose, and maltose). Also, rOnGal8L could regulate inflammatory-related gene expression, phagocytosis, and a respiratory burst of monocytes/macrophages. Moreover, in vivo analysis showed that OnGal8-L overexpression could protect O. niloticus from S. agalactiae infection through modulating serum antibacterial activity (AKP, ACP, and LZM), antioxidant capacity (CAT, POD, and SOD), and monocyte/macrophage proliferation and cytokine expression, as well as reducing bacterial burden and decreasing tissue damage. Our results collectively indicate that OnGal8-L plays important regulatory roles in immune response against bacterial infection.

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“…In P. monodon, both c-type (Ye et al 2009) and i-type (Supungul et al 2010) lysozymes have been identified and shown to have broad antibacterial activities (Tyagi et al 2007). The presence of lysozyme homologs in various tissues of other penaeid shrimps including P. indicus, P. stylirostris and Penaeus penicillatus (Table 4) has been reported, most of which possess antimicrobial activities against bacteria, viruses and fungi (Mai & Wang 2010;Karthik et al 2014;Liao et al 2018;Cai et al 2019).…”

Section: Lysozymesmentioning

confidence: 99%

The expanding repertoire of immune‐related molecules with antimicrobial activity in penaeid shrimps: a review

Aweya

Zheng

et al. 2021

Reviews in Aquaculture

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Shrimps live in a complex aquatic ecosystem with numerous other biotic and abiotic factors, especially pathogenic microorganisms, which require the adaptation of prudent protective and survival strategies. As invertebrates, shrimps reply on humoral and cellular innate immune response mechanisms for protection against pathogens. One integral part of the humoral innate immune defence mechanism involves many biomolecules that are constitutively expressed or induced by pathogens. Shrimps, therefore, produce various biomolecules and secondary metabolites for physiological and pathophysiological purposes, most of which also act as effector molecules in immune-related functions. A growing number of molecules have been identified as essential constituents of the immune arsenal of biomolecules in penaeid shrimps. This review provides a synthesis of important information on penaeid shrimp-encoded immune-related molecules with antimicrobial activities, their molecular regulation and importance in shrimp innate immune response. Research gaps would be identified and proposed research themes emphasized for future studies.

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Identification and functional characterization of a c-type lysozyme from Fenneropenaeus penicillatus

Cited by 25 publications

References 32 publications

The Molecular Mechanism of Hemocyte Immune Response in Marsupenaeus japonicus Infected With Decapod Iridescent Virus 1

The Molecular Mechanism of Hemocyte Immune Response in Marsupenaeus japonicus Infected With Decapod Iridescent Virus 1

Fish Galectin8-Like Exerts Positive Regulation on Immune Response Against Bacterial Infection

The expanding repertoire of immune‐related molecules with antimicrobial activity in penaeid shrimps: a review

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