Identification and functional characterization of the mannose receptor in astrocytes

Burudi, E.M.E.; Riese, Sigrid; Stahl, Philip D.; Régnier‐Vigouroux, Anne

doi:10.1002/(sici)1098-1136(19990101)25:1<44::aid-glia5>3.0.co;2-c

Cited by 70 publications

(62 citation statements)

References 35 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Primary MG cultures were prepared from newborn mouse brains as described by Burudi et al 13 Briefly, the skullcap was opened medially, the brain was carefully removed and transferred into a tube containing 1 Â Hank's balanced salt solution. Meninges were removed under a stereoscope.…”

Section: Materials and Methods Cellsmentioning

confidence: 99%

Activation of a glioma-specific immune response by oncolytic parvovirus Minute Virus of Mice infection

et al. 2012

View full text Add to dashboard Cite

Rodent autonomous parvoviruses (PVs) are endowed with oncotropic properties and represent virotherapeutics with inherent oncolytic features. This work aimed to evaluate the capacity of Minute Virus of Mice (MVMp) to act as an adjuvant stimulating a mouse glioblastoma-specific immune response. MVMp was shown to induce cell death through apoptosis in glioma GL261 cells. Antigen-presenting cells (APCs) provide the initial cue for innate and adaptive immune responses, and thus MVMp-infected GL261 cells were tested for their ability to activate dendritic cells (DCs) and microglia (MG), two distinct cell types that are able to act as APCs. MG and discrete DC subsets were activated after co-culture with MVMp-infected glioma GL261 cells, as evidenced by upregulation of specific activation markers (CD80, CD86) and release of proinflammatory cytokines (tumor necrosis factor-a and interleukin-6). The in vivo analysis of immunodeficient and immunocompetent mice revealed a clear difference in their susceptibility to MVMp-mediated tumor suppression. Immunocompetent mice were fully protected from tumor outgrowth of GL261 cells infected ex vivo with MVMp. In contrast, immunodeficient animals were less competent for MVMp-dependent tumor inhibition, with only 20% of the recipients being protected, arguing for an additional immune component to allow full tumor suppression. In keeping with this conclusion, immunocompetent mice engrafted with MVMp-infected glioma cells developed a level of anti-tumor immunity with isolated splenocytes producing elevated levels of interferon-g. In rechallenge experiments using uninfected GL261 cells, we could show complete protection against the tumor, arguing for the induction of a T-cell-mediated, tumor-specific, long-term memory response. These findings indicate that the anticancer effect of PVs can be traced back not only for their direct oncolytic effect, but also to their ability to break tumor tolerance.

show abstract

Section: Materials and Methods Cellsmentioning

confidence: 99%

Activation of a glioma-specific immune response by oncolytic parvovirus Minute Virus of Mice infection

et al. 2012

View full text Add to dashboard Cite

show abstract

“…Proteins were separated in SDS-polyacrylamide slabs gels using the discontinuous gel and buffer system of Laemmli (1970) with 8% polyacrylamide in the separate gel. After electrophoresis, the proteins were transferred to nitrocellulose paper, which was incubated for 60 min with a rabbit anti-GLUT1 polyclonal antibody (1 : 200), a rabbit anti-MR polyclonal antibody (1 : 5000) (Burudi et al 1999;Burudi and Regnier-Vigouroux 2001) or a rabbit anti-Man II polyclonal antibody (1: 2000) (Moremen and Robbins 1991) in TBS containing 0.05% Tween-20. Thereafter, the paper was incubated for 60 min with an anti-rabbit IgG-AP conjugate.…”

Section: Glut1 Mannose Receptor (Mr) and Mannosidase II (Man Ii) Immmentioning

confidence: 99%

Ethanol impairs monosaccharide uptake and glycosylation in cultured rat astrocytes

Tomàs

Fornas

Megías

et al. 2002

Journal of Neurochemistry

View full text Add to dashboard Cite

Astrocyte and glial-neuron interactions have a critical role in brain development, which is partially mediated by glycoproteins, including adhesion molecules and growth factors. Ethanol affects the synthesis, intracellular transport, subcellular distribution and secretion of these glycoproteins, suggesting alterations in glycosylation. We analyzed the effect of long-term exposure to low doses of ethanol (30 mM) on glycosylation process in growing cultured astrocytes in vitro. Cells were incubated for short (5 min) and long (90 min) periods with several radioactively labeled carbohydrate precursors. The uptake, kinetics and metabolism of these precursors, as well as the radioactivity distribution in protein gels were analyzed. The levels of GLUT1 and mannosidase II were also determined. Ethanol increased the uptake of monosaccharides and the protein levels of GLUT1 but decreased those of mannosidase II. It altered the carbohydrate moiety of proteins and increased cell surface glycoproteins containing terminal non-reduced mannose. These results indicate that ethanol impairs glycosylation in rat astrocytes, thus disrupting brain development.

show abstract

“…However, there is some evidence that under certain conditions astrocytes are capable of phagocytosis (13,14). In contrast to microglia, only SR-BI seems to be expressed by astrocytes from adult mouse and human brains (15), although there are reports suggesting that astrocytes also express the mannose receptor (16). Under pathological conditions, like injury of the nervous system, the activation of astrocytes has been associated with changes in the expression of cytoplasmatic proteins, surface proteins, and soluble factors, such as chemokines (10,12).…”

mentioning

confidence: 99%

Expression of Scavenger Receptors in Glial Cells

Alarcón

Fuenzalida

Santibáñez

et al. 2005

Journal of Biological Chemistry

142

View full text Add to dashboard Cite

Identification and functional characterization of the mannose receptor in astrocytes

Cited by 70 publications

References 35 publications

Activation of a glioma-specific immune response by oncolytic parvovirus Minute Virus of Mice infection

Activation of a glioma-specific immune response by oncolytic parvovirus Minute Virus of Mice infection

Ethanol impairs monosaccharide uptake and glycosylation in cultured rat astrocytes

Expression of Scavenger Receptors in Glial Cells

Contact Info

Product

Resources

About