Identification and functional characterization of a human sTRAIL homolog, CasTRAIL, in an invertebrate oyster Crassostrea ariakensis

Yang, Shoubao; Wu, Xinzhong

doi:10.1016/j.dci.2009.12.014

Cited by 18 publications

(11 citation statements)

References 43 publications

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“…Interactions between protein kinase C, FAK and Src protein tyrosine kinases occur during the integrin-mediated spreading of Lymnaea stagnalis haemocytes [71] and robust intracellular signalling is essential to cytoskeleton remodelling, cell adhesion and migration of PAMP-activated haemocytes [26,72,73]. Although more than 60 MGCs contain a DNA-binding domain and some of them include the SH2 domain (IPR000980), there is no proof in Mytibase of a mussel JAK/STAT pathway, the main signalling system for a wide array of mammalian cytokines and growth factors.…”

Section: Resultsmentioning

confidence: 99%

Insights into the innate immunity of the Mediterranean mussel Mytilus galloprovincialis

et al. 2011

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BackgroundSessile bivalves of the genus Mytilus are suspension feeders relatively tolerant to a wide range of environmental changes, used as sentinels in ecotoxicological investigations and marketed worldwide as seafood. Mortality events caused by infective agents and parasites apparently occur less in mussels than in other bivalves but the molecular basis of such evidence is unknown. The arrangement of Mytibase, interactive catalogue of 7,112 transcripts of M. galloprovincialis, offered us the opportunity to look for gene sequences relevant to the host defences, in particular the innate immunity related genes.ResultsWe have explored and described the Mytibase sequence clusters and singletons having a putative role in recognition, intracellular signalling, and neutralization of potential pathogens in M. galloprovincialis. Automatically assisted searches of protein signatures and manually cured sequence analysis confirmed the molecular diversity of recognition/effector molecules such as the antimicrobial peptides and many carbohydrate binding proteins. Molecular motifs identifying complement C1q, C-type lectins and fibrinogen-like transcripts emerged as the most abundant in the Mytibase collection whereas, conversely, sequence motifs denoting the regulatory cytokine MIF and cytokine-related transcripts represent singular and unexpected findings. Using a cross-search strategy, 1,820 putatively immune-related sequences were selected to design oligonucleotide probes and define a species-specific Immunochip (DNA microarray). The Immunochip performance was tested with hemolymph RNAs from mussels injected with Vibrio splendidus at 3 and 48 hours post-treatment. A total of 143 and 262 differentially expressed genes exemplify the early and late hemocyte response of the Vibrio-challenged mussels, respectively, with AMP trends confirmed by qPCR and clear modulation of interrelated signalling pathways.ConclusionsThe Mytibase collection is rich in gene transcripts modulated in response to antigenic stimuli and represents an interesting window for looking at the mussel immunome (transcriptomes mediating the mussel response to non-self or abnormal antigens). On this basis, we have defined a new microarray platform, a mussel Immunochip, as a flexible tool for the experimental validation of immune-candidate sequences, and tested its performance on Vibrio-activated mussel hemocytes. The microarray platform and related expression data can be regarded as a step forward in the study of the adaptive response of the Mytilus species to an evolving microbial world.

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Section: Resultsmentioning

confidence: 99%

Insights into the innate immunity of the Mediterranean mussel Mytilus galloprovincialis

et al. 2011

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“…Other ligands related to the apoptotic process, such as the TRAIL (Apo2-ligand) and the FasL ligand, have 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52 53 54 55 56 57 58 59 60 61 62 63 64 been identified in Crassostrea ariakensis, C. gigas, O. edulis and H. discus discus [22,84,143,147,148]. Transmembrane receptors, such as TRAF and TTRAP proteins, were described in C. farreri [102,142], and adaptor proteins similar to human FADD were identified in C. gigas [147,148] (Fig.…”

Section: Caspase Activation: the Extrinsic Pathwaymentioning

confidence: 97%

The complexity of apoptotic cell death in mollusks: An update

Romero

Novoa

Figueras

2015

Fish & Shellfish Immunology

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“…[30] and modified by Yang [15]. Briefly, hemocytes suspensions were settle onto slides and incubated in a humid chamber at room temperature for 15 min.…”

Section: Immunofluorescence and Immunoelectron Microscopy Of The Tetrmentioning

confidence: 99%

Molecular characterization of a novel tetraspanin from the oyster, Crassostrea ariakensis: Variation, localization and relationship to oyster host defense

Luo¹,

Ye²,

Xu³

et al. 2012

Fish & Shellfish Immunology

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Identification and functional characterization of a human sTRAIL homolog, CasTRAIL, in an invertebrate oyster Crassostrea ariakensis

Cited by 18 publications

References 43 publications

Insights into the innate immunity of the Mediterranean mussel Mytilus galloprovincialis

Insights into the innate immunity of the Mediterranean mussel Mytilus galloprovincialis

The complexity of apoptotic cell death in mollusks: An update

Molecular characterization of a novel tetraspanin from the oyster, Crassostrea ariakensis: Variation, localization and relationship to oyster host defense

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