Identification and functional characterization of a novel C-type lectin from the kuruma shrimp, Marsupenaeus japonicus

Zheng, Jinbin; Mao, Yong; Su, Yongquan

doi:10.1016/j.bbrc.2020.07.067

Cited by 19 publications

(4 citation statements)

References 40 publications

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“…C-Type lectin (CTL), a pattern recognition receptor, plays an essential role in immune reactions such as bacterial and viral defense, phagocytosis, cell adhesion, prophenoloxidase activation, etc. [39,40]. In this study, increased expression levels of ALF1, ALF2, C-Lectin, Crustin, and Lysozyme were noticeable after A. veronii infection.…”

Section: Discussionsupporting

confidence: 53%

Pathogenicity of Aeromonas veronii Isolated from Diseased Macrobrachium rosenbergii and Host Immune-Related Gene Expression Profiles

Gao,

Chen,

Zhang

et al. 2024

Microorganisms

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Aeromonas veronii is widespread in aquatic environments and is responsible for infecting various aquatic animals. In this study, a dominant strain was isolated from the hepatopancreas of diseased Macrobrachium rosenbergii and was named JDM1-1. According to its morphological, physiological, and biochemical characteristics and molecular identification, isolate JDM1-1 was identified as A. veronii. The results of artificial challenge showed isolate JDM1-1 had high pathogenicity to M. rosenbergii with an LD50 value of 8.35 × 105 CFU/mL during the challenge test. Histopathological analysis revealed severe damage in the hepatopancreas and gills of the diseased prawns, characterized by the enlargement of the hepatic tubule lumen and gaps between the tubules as well as clubbing and degeneration observed at the distal end of the gill filament. Eight virulence-related genes, namely aer, ompA, lip, tapA, hlyA, flgA, flgM, and flgN, were screened by PCR assay. In addition, virulence factor detection showed that the JDM1-1 isolate produced lipase, lecithinase, gelatinase, and hemolysin. Furthermore, the mRNA expression profiles of immune-related genes of M. rosenbergii following A. veronii infection, including ALF1, ALF2, Crustin, C-lectin, and Lysozyme, were assessed, and the results revealed a significant upregulation in the hepatopancreas and intestines at different hours post infection. This study demonstrates that A. veronii is a causative agent associated with massive die-offs of M. rosenbergii and contributes valuable insights into the pathogenesis and host defense mechanisms of A. veronii invasion.

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Section: Discussionsupporting

confidence: 53%

Pathogenicity of Aeromonas veronii Isolated from Diseased Macrobrachium rosenbergii and Host Immune-Related Gene Expression Profiles

Gao,

Chen,

Zhang

et al. 2024

Microorganisms

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“…The VP24-truncated isolate of white spot syndrome virus possessed lower infectivity in viral infection [28] . Furthermore, several previous studies suggested that VP24 interacted with several pathogen-associated molecular pattern during WSSV infection [29] , [30] , [31] . Herein, we discovered two highly homologous lysozyme from crayfish.…”

Section: Discussionmentioning

confidence: 94%

Identification and characterization of two highly homologous lysozymes from red swamp crayfish, Procambarus clarkii

Yin

Li-Chao

et al. 2021

Fish and Shellfish Immunology Reports

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“…The recombinant proteins (including the individual CRDs) were able to agglutinate microorganisms in a Ca 2+ ‐independent or Ca 2+ ‐dependent manner eliciting its PRR functions 79–81 . Additionally, transcriptome analysis in crustacean models, including P. trituberculatus (four CTLs) 82 and P. japonicus (six CTLs), 83 has improved the repertoire of CTLs gaining insights into the transcript variants and their immune activity. The functions of CTL in shrimp and other crustaceans defence response to pathogens have been reviewed concerning PRR specificity 5,9,84 .…”

Section: Prrs In Crustaceansmentioning

confidence: 99%

The role of pattern recognition receptors in crustacean innate immunity

Patnaik

Baliarsingh

Sarkar³

et al. 2023

Reviews in Aquaculture

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Crustacean species are among the most valued seafood with socioeconomic and ecological implications in the global fisheries sector. Microbial infections to the farming stock due to continued expansion and aquaculture puts global crustacean production at risk. Understanding and reviewing the complex innate immune system of crustaceans would be pivotal in formulating informed decisions for the control and management of epidemic diseases, thus making the global crustacean aquaculture industry sustainable. Under the purview of innate immunity in crustaceans, pattern recognition receptors (PRRs) represent the sensors capable of discriminating non‐self pathogenic modules and invoking the activation of immune responses. The immunity to infections can be manifested at cellular (via phagocytosis, apoptosis, nodulation and encapsulation) and humoral (effected by prophenoloxidase cascade, lectins, antimicrobial proteins) levels leading to the elimination of pathogens or subversion of the pathogenic implications. In this review, we discuss the participatory role of 12 types of PRRs identified from crustacean species with an exhaustive focus on structural and functional specificities in binding to pathogens and possible execution of the immune activity. Moreover, we have elaborated on the PRRs‐associated or possible signalling cascades (Toll‐NFκB, IMD‐NFκB and JAK‐STAT) in crustacean species that could bridge our understanding of the resistance or susceptibility of the host to varied pathogenic infections thus providing clues to the regulatory mechanisms involved in pathogenesis of infectious diseases and mechanisms of immunity.

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Identification and functional characterization of a novel C-type lectin from the kuruma shrimp, Marsupenaeus japonicus

Cited by 19 publications

References 40 publications

Pathogenicity of Aeromonas veronii Isolated from Diseased Macrobrachium rosenbergii and Host Immune-Related Gene Expression Profiles

Pathogenicity of Aeromonas veronii Isolated from Diseased Macrobrachium rosenbergii and Host Immune-Related Gene Expression Profiles

Identification and characterization of two highly homologous lysozymes from red swamp crayfish, Procambarus clarkii

The role of pattern recognition receptors in crustacean innate immunity

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