Identification and functional characterization of the human T-cell receptor beta gene transcriptional enhancer: common nuclear proteins interact with the transcriptional regulatory elements of the T-cell receptor alpha and beta genes.

Gottschalk, Lisa; Leiden, Jeffrey M.

doi:10.1128/mcb.10.10.5486

Cited by 111 publications

(139 citation statements)

References 43 publications

Supporting

Mentioning

129

Contrasting

Order By: Relevance

“…(29,30). Although these sites were identified earlier, a consensus opinion on their function in the TCR␤ enhancer had proven hard to establish (20,21) until the recent studies of the human TCR␤ enhancer (23). Our observations conclusively demonstrate that individual elements of both composite motifs are essential for activity of the murine TCR␤ enhancer.…”

Section: Figuresupporting

confidence: 64%

“…Alignment of the murine and human ␤ enhancer sequences show that the murine ␤E1 and ␤E2 motifs (including a GATA site) fall within T␤2, ␤E4 falls within T␤3, ␤E5 and ␤E6 fall within T␤4, and ␤E7 falls within T␤5. Mutations or deletion of T␤2 and T␤3 reduced enhancer 30 -50% (20). Deletions of T␤4 gave more variable results, reducing activity to 10% (20) or 40% (21) in two different studies.…”

Section: Functional Analysis Of the Murine Tcr␤-chain Gene Enhancer 1mentioning

confidence: 99%

“…Mutations or deletion of T␤2 and T␤3 reduced enhancer 30 -50% (20). Deletions of T␤4 gave more variable results, reducing activity to 10% (20) or 40% (21) in two different studies. Recently, Kim et al (23) showed that mutation of individual ETS and CBF elements of the human TCR␤ enhancer significantly decreased activity in BW5147 cells.…”

Section: Functional Analysis Of the Murine Tcr␤-chain Gene Enhancer 1mentioning

confidence: 99%

“…Five regions (T␤1-T␤5) of a 480-bp fragment of the human ␤ enhancer were shown to bind nuclear factors by DNaseI footprinting assays (20). In addition, Prosser et al (22) previously identified closely juxtaposed binding sites for ETS domain proteins and core binding factor (CBF/AML1/PEBP2) in the T␤3 and T␤4 motifs of the human TCR␤ enhancer.…”

Section: Functional Analysis Of the Murine Tcr␤-chain Gene Enhancer 1mentioning

confidence: 99%

“…The human TCR␤ enhancer has also been identified and characterized (20,21). Five regions (T␤1-T␤5) of a 480-bp fragment of the human ␤ enhancer were shown to bind nuclear factors by DNaseI footprinting assays (20).…”

Section: Functional Analysis Of the Murine Tcr␤-chain Gene Enhancer 1mentioning

confidence: 99%

See 4 more Smart Citations

Functional Analysis of the Murine TCRβ-Chain Gene Enhancer

Carvajal

Sen

2000

The Journal of Immunology

View full text Add to dashboard Cite

The TCRβ-chain gene enhancer activates transcription and V(D)J recombination in immature thymocytes. In this paper we present a systematic analysis of the elements that contribute to the activity of the murine TCRβ enhancer in mature and immature T cell lines. We identified a region containing the βE4, βE5, and βE6 motifs as the essential core of the TCRβ enhancer in pro-T cells. In mature cells, the core enhancer had low activity and required, in addition, either 5′ or 3′ flanking sequences whose functions may be partially overlapping. Mutation of any of the six protein binding sites located within the βE4–βE6 elements essentially abolished enhancer activity, indicating that this core enhancer contained no redundant elements. The βE4 and βE6 elements contain binding sites for ETS-domain proteins and the core binding factor. The βE5 element bound two proteins that could be resolved chromatographically and that were both essential for enhancer activity.

show abstract

Section: Figuresupporting

confidence: 64%

Section: Functional Analysis Of the Murine Tcr␤-chain Gene Enhancer 1mentioning

confidence: 99%

Section: Functional Analysis Of the Murine Tcr␤-chain Gene Enhancer 1mentioning

confidence: 99%

Section: Functional Analysis Of the Murine Tcr␤-chain Gene Enhancer 1mentioning

confidence: 99%

Section: Functional Analysis Of the Murine Tcr␤-chain Gene Enhancer 1mentioning

confidence: 99%

See 3 more Smart Citations

Functional Analysis of the Murine TCRβ-Chain Gene Enhancer

Carvajal

Sen

2000

The Journal of Immunology

View full text Add to dashboard Cite

show abstract

Interrelationships of nuclear structure and transcriptional control: Functional consequences of being in the right place at the right time

et al. 1998

View full text Add to dashboard Cite

Functional interrelationships between components of nuclear architecture and control of gene expression are becoming increasingly evident. In this article we focus on the concept that association of genes and cognate transcription factors with the nuclear matrix may support the formation and/or activities of nuclear domains that facilitate transcriptional regulation. Several lines of evidence are consistent with the concept that association of transcription factors with the nuclear matrix may be obligatory for fidelity of gene expression and maximal transcriptional activity. The identification of specific regions of transcription factors that are responsible for intranuclear trafficking to nuclear matrix-associated sites that support transcription, reinforces the linkage of nuclear structure to regulation of genes. CBFA2/AML-1 and CBFA1/AML-3 provide paradigms for directing gene regulatory factors to RNA polymerase II containing foci within the nucleus. The implications of modifications in the intranuclear trafficking of transcription factors for developmental and tissue-specific control, as well as for aberrations in gene expression that are associated with cancer and neurological disorders, are addressed.

show abstract