Identification and Functional Characterization of Two Highly Divergent Aryl Hydrocarbon Receptors (AHR1 and AHR2) in the TeleostFundulus heteroclitus

Karchner, Sibel I.; Powell, Wade H.; Hahn, Mark E.

doi:10.1074/jbc.274.47.33814

Cited by 154 publications

(169 citation statements)

References 91 publications

(93 reference statements)

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“…Gradients were centrifuged in a vertical tube rotor at 327,398 x g for 2 h at 4 °C, and fractionated (Brandel Gaithersburg, MD) to approximately 120 μL/fraction (0.750 mL/min). Fractions were counted by liquid scintillation; the pattern of specific binding of [ 3 H]TCDD was similar to that reported by Karchner et al (1999). Fractions 10-19 were collected and pooled for each of the TCDF-containing AHR1, AHR2, and UPL gradients processed in parallel to the [ 3 H]TCDD gradients.…”

Section: Purification Of In Vitro-expressed Proteinsmentioning

confidence: 87%

“…Karchner et al (1999) reported that killifish AHR1 mRNA was most highly expressed in heart, brain and ovary with lower expression in liver, kidney, and gill. Our results suggest that the AHR1 protein is more widely expressed than mRNA expression levels would suggest.…”

Section: Discussionmentioning

confidence: 99%

“…In vitroexpressed AHR1, AHR2, and unprogrammed lysate (TNT reactions with empty vector, UPL) were incubated overnight with TCDF (Karchner et al 2002). To identify the AHR-containing fractions, additional tubes were prepared with AHR proteins incubated with 2 nM of the radioligand [ 3 H]TCDD (Tsui and Okey 1981;Karchner et al 1999).…”

Section: Purification Of In Vitro-expressed Proteinsmentioning

confidence: 99%

“…Several killifish genes in the AHR signaling pathway have been characterized, including CYP1A (Morrison et al 1998), ARNT2 , AHR1 and AHR2 (Hahn et al 1997;Karchner et al 1999;Hahn et al 2004), and AHRR (Karchner et al 2002). Both killifish AHR1 and AHR2 bind TCDD and other AHR ligands in vitro and stimulate transactivation of reporter genes regulated by the CYP1A promoter in cultured mammalian cells.…”

Section: Introductionmentioning

confidence: 99%

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Development and characterization of polyclonal antibodies against the aryl hydrocarbon receptor protein family (AHR1, AHR2, and AHR repressor) of Atlantic killifish Fundulus heteroclitus

Merson

Franks

Karchner

et al. 2006

Comparative Biochemistry and Physiology Part C: Toxicology &

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The aryl hydrocarbon receptor (AHR) and AHR repressor (AHRR) proteins regulate gene expression in response to some halogenated aromatic hydrocarbons and polycyclic aromatic hydrocarbons. The Atlantic killifish is a valuable model of the AHR signaling pathway, but antibodies are not available to fully characterize AHR and AHRR proteins. Using bacterially expressed AHRs, we developed specific and sensitive polyclonal antisera against the killifish AHR1, AHR2, and AHRR. In immunoblots, these antibodies recognized full-length killifish AHR and AHRR proteins synthesized in rabbit reticulocyte lysate, proteins expressed in mammalian cells transfected with killifish AHR and AHRR constructs, and AHR proteins in cytosol preparations from killifish tissues. Killifish AHR1 and AHR2 proteins were detected in brain, gill, kidney, heart, liver, and spleen. Antisera specifically precipitated their respective target proteins in immunoprecipitation experiments with in vitro-expressed proteins. Killifish ARNT2 co-precipitated with AHR1 and AHR2. These sensitive, specific, and versatile antibodies will be valuable to researchers investigating AHR signaling and other physiological processes involving AHR and AHRR proteins.

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Section: Purification Of In Vitro-expressed Proteinsmentioning

confidence: 87%

Section: Discussionmentioning

confidence: 99%

Section: Purification Of In Vitro-expressed Proteinsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Development and characterization of polyclonal antibodies against the aryl hydrocarbon receptor protein family (AHR1, AHR2, and AHR repressor) of Atlantic killifish Fundulus heteroclitus

Merson

Franks

Karchner

et al. 2006

Comparative Biochemistry and Physiology Part C: Toxicology &

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“…; 2014). In light of the key role of AHR in controlling sensitivity in a variety of vertebrates, an initial focus was on AHR itself as a candidate resistance gene, a hypothesis complicated by the identification of multiple killifish AHRs (Karchner, Powell, & Hahn, 1999). Several polymorphic variants of both AHR1 and AHR2 were identified, and both loci (now known as AHR1a and AHR2a ) showed signals of selection in the NBH population (Hahn, Karchner, Franks, & Merson, 2004; Reitzel et al., 2014).…”

Section: Genetic Basis Of Pollution Tolerance In Killifishmentioning

confidence: 99%

When evolution is the solution to pollution: Key principles, and lessons from rapid repeated adaptation of killifish (Fundulus heteroclitus) populations

Whitehead

Clark

Reid

et al. 2017

Evolutionary Applications

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For most species, evolutionary adaptation is not expected to be sufficiently rapid to buffer the effects of human‐mediated environmental changes, including environmental pollution. Here we review how key features of populations, the characteristics of environmental pollution, and the genetic architecture underlying adaptive traits, may interact to shape the likelihood of evolutionary rescue from pollution. Large populations of Atlantic killifish (Fundulus heteroclitus) persist in some of the most contaminated estuaries of the United States, and killifish studies have provided some of the first insights into the types of genomic changes that enable rapid evolutionary rescue from complexly degraded environments. We describe how selection by industrial pollutants and other stressors has acted on multiple populations of killifish and posit that extreme nucleotide diversity uniquely positions this species for successful evolutionary adaptation. Mechanistic studies have identified some of the genetic underpinnings of adaptation to a well‐studied class of toxic pollutants; however, multiple genetic regions under selection in wild populations seem to reflect more complex responses to diverse native stressors and/or compensatory responses to primary adaptation. The discovery of these pollution‐adapted killifish populations suggests that the evolutionary influence of anthropogenic stressors as selective agents occurs widely. Yet adaptation to chemical pollution in terrestrial and aquatic vertebrate wildlife may rarely be a successful “solution to pollution” because potentially adaptive phenotypes may be complex and incur fitness costs, and therefore be unlikely to evolve quickly enough, especially in species with small population sizes.

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Impacts of Dioxin‐Activated AHR Signaling in Fish and Birds

Simonich

Tanguay

2011

The AH Receptor in Biology and Toxicology

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Identification and Functional Characterization of Two Highly Divergent Aryl Hydrocarbon Receptors (AHR1 and AHR2) in the TeleostFundulus heteroclitus

Cited by 154 publications

References 91 publications

Development and characterization of polyclonal antibodies against the aryl hydrocarbon receptor protein family (AHR1, AHR2, and AHR repressor) of Atlantic killifish Fundulus heteroclitus

Development and characterization of polyclonal antibodies against the aryl hydrocarbon receptor protein family (AHR1, AHR2, and AHR repressor) of Atlantic killifish Fundulus heteroclitus

When evolution is the solution to pollution: Key principles, and lessons from rapid repeated adaptation of killifish (Fundulus heteroclitus) populations

Impacts of Dioxin‐Activated AHR Signaling in Fish and Birds

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