2002
DOI: 10.1074/jbc.m108747200
|View full text |Cite
|
Sign up to set email alerts
|

Identification and Functional Characterization of an Intragenic DNA Binding Site for the Spumaretroviral trans-Activator in the Human p57 Gene

Abstract: Expression of the human cyclin-dependent protein kinase inhibitor p57Kip2 gene was previously shown to be specifically and strongly activated by the retroviral trans-activator Bel1 of human foamy virus by means of expression profiling, Northern, and Western blot analysis. Here we report that Bel1-mediated trans-activation was conferred by a Bel1 response element (BRE) located in the second exon of p57Kip2 . The intragenic Kip2-BRE was capable of trans-activating the luciferase reporter gene upon cotransfection… Show more

Help me understand this report

Search citation statements

Order By: Relevance

Paper Sections

Select...
2

Citation Types

0
16
0
1

Year Published

2003
2003
2016
2016

Publication Types

Select...
3
2
1

Relationship

1
5

Authors

Journals

citations
Cited by 11 publications
(17 citation statements)
references
References 31 publications
(32 reference statements)
0
16
0
1
Order By: Relevance
“…Plasmids pUC18, pCMV␤-gal, pbel1s (24), pGL3-basic-HFV-IP (Ϫ192 to Ϫ1), pGL3-pro-IP.BRE Ϫ166 to Ϫ116 and Ϫ166 to Ϫ140 derivatives (23) were transfected into 293T cells using the coprecipitation method of Chen and Okayama (25). In general, 6 g of plasmid DNA were transfected into 293T cells grown in 6-cm Petri dishes.…”
Section: Methodsmentioning
confidence: 99%
See 4 more Smart Citations
“…Plasmids pUC18, pCMV␤-gal, pbel1s (24), pGL3-basic-HFV-IP (Ϫ192 to Ϫ1), pGL3-pro-IP.BRE Ϫ166 to Ϫ116 and Ϫ166 to Ϫ140 derivatives (23) were transfected into 293T cells using the coprecipitation method of Chen and Okayama (25). In general, 6 g of plasmid DNA were transfected into 293T cells grown in 6-cm Petri dishes.…”
Section: Methodsmentioning
confidence: 99%
“…Identical amounts of proteins were separated by SDS-PAGE, blotted, reacted with monoclonal serum directed against the HA epitope of the four different HA-tagged NFI proteins (Roche) or against hNFI-X1 protein (sc870X, Santa Crux), or polyclonal sera directed against Bel1/Bet, Gag, or RNase H proteins (23), and detected by enhanced chemiluminescence as described previously (23).…”
Section: Methodsmentioning
confidence: 99%
See 3 more Smart Citations