Identification and Functional Characterization of Ion Channels in CD34+ Hematopoietic Stem Cells from Human Peripheral Blood

Park, Kyoung Sun; Pang, Bo; Park, Su Jung; Lee, Yun Gyoo; Bae, Ji‐Young; Park, Wan Beom; Kim, Inho; Kim, Sung-Joon

doi:10.1007/s10059-011-0068-9

Cited by 18 publications

(13 citation statements)

References 34 publications

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“…The composition and potential use of cAMSC-CM have not been studied yet, but since CM contains cell paracrine factors [ 37 ], the application of cAMSC-CM in clinical studies seems to be promising. In our study, cAMSC phenotype was confirmed through FACS analysis and showed a high expression of CD29, CD44, and CD90 surface markers, whereas CD34 and CD45 expression, which are associated with hematopoietic stem cells [ 57 , 58 ], was low ( Figure 1 ). This phenotype has also been found in other studies using canine MSCs [ 59 , 60 , 61 ], and CD29, CD44, and CD90 surface antigens are found on adipose, bone marrow, and umbilical cord blood-derived mesenchymal stem cells [ 62 , 63 ].…”

Section: Discussionsupporting

confidence: 61%

Conditioned Medium from Canine Amniotic Membrane-Derived Mesenchymal Stem Cells Improved Dog Sperm Post-Thaw Quality-Related Parameters

Mahiddine

Kim

Qamar

et al. 2020

Animals

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This study investigated the effects of conditioned medium (CM) from canine amniotic membrane-derived MSCs (cAMSCs) on dog sperm cryopreservation. For this purpose, flow cytometry analysis was performed to characterize cAMSCs. The CM prepared from cAMSCs was subjected to proteomic analysis for the identification of proteins present in the medium. Sperm samples were treated with freezing medium supplemented with 0%, 5%, 10%, and 15% of the CM, and kinetic parameters were evaluated after 4–6 h of chilling at 4 °C to select the best concentration before proceeding to cryopreservation. Quality-related parameters of frozen–thawed sperm were investigated, including motility; kinetic parameters; viability; integrity of the plasma membrane, chromatin, and acrosome; and mitochondrial activity. The results showed that 10% of the CM significantly enhanced motility, viability, mitochondrial activity, and membrane integrity (p < 0.05); however, the analysis of chromatin and acrosome integrity showed no significant differences between the treatment and control groups. Therefore, we concluded that the addition of 10% CM derived from cAMSC in the freezing medium protected dog sperm during the cryopreservation process.

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Section: Discussionsupporting

confidence: 61%

Conditioned Medium from Canine Amniotic Membrane-Derived Mesenchymal Stem Cells Improved Dog Sperm Post-Thaw Quality-Related Parameters

Mahiddine

Kim

Qamar

et al. 2020

Animals

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“…55 A plausible mechanism would be that maturation of HSCs is voltage regulated. HSCs were found to express mRNA for voltage-gated potassium channels Kv1.3, Kv7.1, 56 and Kv2.1, 57 rendering these cells responsive to voltage-gated potassium efflux, which results in calcium influx. 58 Electrical pulsed currents activate homing of EPCs and other progenitor cells to the site of injury in vivo and thereby increase perfusion and drive regeneration of hypoxia-damaged tissue.…”

Section: Discussionmentioning

confidence: 99%

Electrical Muscle Stimulation Induces an Increase of VEGFR2 on Circulating Hematopoietic Stem Cells in Patients With Diabetes

Hidmark

Spanidis

Fleming

et al. 2017

Clinical Therapeutics

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Three EMS treatments decreased symptoms of pain caused by DN and reduced diastolic blood pressure and biomarkers of stress. A single EMS treatment increased molecules mediating attachment and differentiation on the surface of HSCs in circulation. We hypothesize that the EMS-induced increase in surface attachment molecules on the HSCs caused the HSCs to leave circulation and that EMS treatment improves the function of HSCs and EPCs in vivo.

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“…Equally important to the role metal ions play within the niche are the metal ion channels that are expressed by HSC. HSC express various ion channels including the P2 family of purinergic receptors (reviewed in [Feng et al, ]) and various K + channels [Park et al, ]. Specifically, HSC express both inwardly rectifying K + channels (K ir ) [Shirihai et al, ] and hERG K + channels [Pillozzi et al, ], which have been implicated in cytokine induced HSC proliferation and differentiation.…”

Section: Non‐protein Metabolitesmentioning

confidence: 99%

Niche Extracellular Matrix Components and Their Influence on HSC

et al. 2017

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Maintenance of hematopoietic stem cells (HSC) takes place in a highly specialized microenvironment within the bone marrow. Technological improvements, especially in the field of in vivo imaging, have helped unravel the complexity of the niche microenvironment and have completely changed the classical concept from what was previously believed to be a static supportive platform, to a dynamic microenvironment tightly regulating HSC homeostasis through the complex interplay between diverse cell types, secreted factors, extracellular matrix molecules, and the expression of different transmembrane receptors. To add to the complexity, non-protein based metabolites have also been recognized as a component of the bone marrow niche. The objective of this review is to discuss the current understanding on how the different extracellular matrix components of the niche regulate HSC fate, both during embryonic development and in adulthood. Special attention will be provided to the description of non-protein metabolites, such as lipids and metal ions, which contribute to the regulation of HSC behavior. J. Cell. Biochem. 118: 1984-1993, 2017. © 2017 Wiley Periodicals, Inc.

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Identification and Functional Characterization of Ion Channels in CD34+ Hematopoietic Stem Cells from Human Peripheral Blood

Cited by 18 publications

References 34 publications

Conditioned Medium from Canine Amniotic Membrane-Derived Mesenchymal Stem Cells Improved Dog Sperm Post-Thaw Quality-Related Parameters

Conditioned Medium from Canine Amniotic Membrane-Derived Mesenchymal Stem Cells Improved Dog Sperm Post-Thaw Quality-Related Parameters

Electrical Muscle Stimulation Induces an Increase of VEGFR2 on Circulating Hematopoietic Stem Cells in Patients With Diabetes

Niche Extracellular Matrix Components and Their Influence on HSC

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