Identification and functional characterization of pfm, a novel gene involved in swimming motility of Pseudomonas aeruginosa

Bai, Fang; Li, Yingli; Xu, Haijing; Xia, Huiming; Yin, Tao; Yao, Hongming; Zhang, Lu; Zhang, Xiuming; Bai, Yanling; Jin, Shouguang; Qiao, Mingqiang

doi:10.1016/j.gene.2007.06.019

Cited by 12 publications

(17 citation statements)

References 33 publications

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“…However, when the incubation time was extended to 48 h, it was able to swim and form a typical swimming pattern, although the average swimming diameter of strain PAO170 (Δ fabV ) was much smaller than that of the wild-type strain and the fabI mutant strain PAO272 (Δ fabI ; Figure 3B ). This result was not consistent with a previous study, in which Bai et al (2007) reported that mini-Mu insertion in fabV caused the PA68 strain of P. aeruginosa to lose swimming motility. However, it is important to note that, when Bai et al (2007) carried out the swimming assay for the fabV strain, they incubated the mutant strain for only 16 h on a swimming assay plate.…”

Section: Resultscontrasting

confidence: 99%

“…This result was not consistent with a previous study, in which Bai et al (2007) reported that mini-Mu insertion in fabV caused the PA68 strain of P. aeruginosa to lose swimming motility. However, it is important to note that, when Bai et al (2007) carried out the swimming assay for the fabV strain, they incubated the mutant strain for only 16 h on a swimming assay plate. Thus, we speculate that mutation of fabV did not inhibit P. aeruginosa swimming motility directly, but led to slow growth and therefore caused it to form the swimming pattern late.…”

Section: Resultscontrasting

confidence: 99%

“…In P. aeruginosa , FabV also exhibits pleiotropic effects (Bai et al, 2007, 2011; Mou et al, 2011). The fabV (formerly named pfm ) gene was first identified to be required for swimming motility, and it was suggested that FabV was involved in energy metabolism, which is critical for the rotation of the flagellum in P. aeruginosa (Bai et al, 2007).…”

Section: Introductionmentioning

confidence: 99%

“…The fabV (formerly named pfm ) gene was first identified to be required for swimming motility, and it was suggested that FabV was involved in energy metabolism, which is critical for the rotation of the flagellum in P. aeruginosa (Bai et al, 2007). Further studies showed that fabV was involved in bacterial protein secretion and bacterial adherence, and mutation of fabV caused P. aeruginosa to produce significantly fewer QS signal molecules and reduced the pathogenicity and virulence in a murine model of acute lung infection.…”

Section: Introductionmentioning

confidence: 99%

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Functional Characterization of Triclosan-Resistant Enoyl-acyl-carrier Protein Reductase (FabV) in Pseudomonas aeruginosa

Huang

Lin

et al. 2016

Front. Microbiol.

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Pseudomonas aeruginosa is extremely resistant to triclosan. Previous studies have shown that P. aeruginosa encodes a triclosan-resistant enoyl-acyl-carrier protein reductase (ENR), FabV, and that deletion of fabV causes P. aeruginosa to be extremely sensitive to triclosan. In this report, we complemented a P. aeruginosa fabV deletion strain with several triclosan-resistant ENR encoding genes, including Vibrio cholerae fabV, Bacillus subtilis fabL and Enterococcus faecalis fabK. All complemented strains restored triclosan resistance to the level of the wild-type strain, which confirmed that triclosan-resistant ENR allows P. aeruginosa to be extremely resistant to triclosan. Moreover, fabV exhibits pleiotropic effects. Deletion of fabV led P. aeruginosa to show attenuated swarming motility, decreased rhamnolipid, pyoverdine and acyl-homoserine lactones (AHLs) production. Complementation of the fabV mutant with any one ENR encoding gene could restore these features to some extent, in comparison with the wild-type strain. Furthermore, we found that addition of exogenous AHLs could restore the fabV mutant strain to swarm on semisolid plates and to produce more virulence factors than the fabV mutant strain. These findings indicate that deletion of fabV reduced the activity of ENR in P. aeruginosa, decreased fatty acid synthesis, and subsequently depressed the production of AHLs and other virulence factors, which finally may led to a reduction in the pathogenicity of P. aeruginosa. Therefore, fabV should be an ideal target for the control of P. aeruginosa infectivity.

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Section: Resultscontrasting

confidence: 99%

Section: Resultscontrasting

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Functional Characterization of Triclosan-Resistant Enoyl-acyl-carrier Protein Reductase (FabV) in Pseudomonas aeruginosa

Huang

Lin

et al. 2016

Front. Microbiol.

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“…A series of virulence factors were tested according to previously described methods including pyocyanin production (Kurachi, 1958), protease assay (Brown and Foster, 1970;Wretlind et al, 1977;Sokol et al, 1979), hemolysin assay (Bettelheim, 1995), swimming (Bai et al, 2007;Wilhelm et al, 2007;Overhage et al, 2008), swarming (Kohler et al, 2000;Overhage et al, 2008) and twitching motility (Alm and Mattick, 1995;Semmler et al, 1999) in both PAO(muxA -) and PAO1. Twitching motility was tested following the method described previously (Alm and Mattick, 1995;Semmler et al, 1999).…”

Section: M9mentioning

confidence: 99%

Inactivation of MuxABC-OpmB transporter system in Pseudomonas aeruginosa leads to increased ampicillin and carbenicillin resistance and decreased virulence

et al. 2011

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Resistance-Nodulation-Cell Division (RND) pumps play important roles in bacterial resistance to antibiotics. Pseudomonas aeruginosa is an important human pathogen which exhibits high level resistance to antibiotics. There are total of 12 RND pumps present in the P. aeruginosa PAOl genome. The recently characterized MuxABC-OpmB system has been shown to play a role in resistance to novobiocin, aztreonam, macrolides, and tetracycline in a multiple knockout mutation. In this study, we examined the expression levels of all the 12 RND pump gene clusters and tested the involvement of MuxABC-OpmB in pathogenicity. The results indicated that in addition to the four known constitutively expressed RND pumps, mexAB-oprM, mexGHI-opmD, mexVW, and mexXY, relatively high levels of expression were observed with mexJK and muxABC-opmB in the conditions tested. Inactivation of muxA in the muxABC-opmB operon resulted in elevated resistance to ampicillin and carbenicillin. The mutant also showed attenuated virulence in both Brassica rapa pekinensis and Drosophila melanogaster infection models. The decreased virulence at least in part was due to decreased twitching motility in the mutant. These results indicate that the RND pump MuxABC-OpmB is associated with ampicillin and carbenicillin susceptibility and also involved in pathogenesis in P. aeruginosa.

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The cold adaption profiles of Pseudoalteromonas shioyasakiensis D1497 from Yap trench to cope with cold

Duan

Guo

2021

Biotechnology Reports

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Highlights P. shioyasakiensis D1497converted more substrate into biomass at low temperature. P. shioyasakiensis D1497 prefered to use codons with A/T in the third position. The energy metabolism related genes were down regulated in cold environment. P. shioyasakiensis D1497 presented an energy saving metabolism strategy to cope with cold.

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Identification and functional characterization of pfm, a novel gene involved in swimming motility of Pseudomonas aeruginosa

Cited by 12 publications

References 33 publications

Functional Characterization of Triclosan-Resistant Enoyl-acyl-carrier Protein Reductase (FabV) in Pseudomonas aeruginosa

Functional Characterization of Triclosan-Resistant Enoyl-acyl-carrier Protein Reductase (FabV) in Pseudomonas aeruginosa

Inactivation of MuxABC-OpmB transporter system in Pseudomonas aeruginosa leads to increased ampicillin and carbenicillin resistance and decreased virulence

The cold adaption profiles of Pseudoalteromonas shioyasakiensis D1497 from Yap trench to cope with cold

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