Identification and functional characterization of muscle satellite cells in Drosophila

Abstract

Work on genetic model systems such as Drosophila and mouse has shown that the fundamental mechanisms of myogenesis are remarkably similar in vertebrates and invertebrates. Strikingly, however, satellite cells, the adult muscle stem cells that are essential for the regeneration of damaged muscles in vertebrates, have not been reported in invertebrates. In this study, we show that lineal descendants of muscle stem cells are present in adult muscle of Drosophila as small, unfused cells observed at the surface and in close proximity to the mature muscle fibers. Normally quiescent, following muscle fiber injury, we show that these cells express Zfh1 and engage in Notch-Delta-dependent proliferative activity and generate lineal descendant populations, which fuse with the injured muscle fiber. In view of strikingly similar morphological and functional features, we consider these novel cells to be the Drosophila equivalent of vertebrate muscle satellite cells.

The African turquoise killifish: A research organism to study vertebrate aging and diapause

“…( 2017), Tropini, Earle, Huang and Sonnenburg ( 2017); excretory system: King and Goldstein (1985), Buechner (2002), Gautam, Verma and Tapadia ( 2017); sleep and circadian system: Raizen et al. ( 2008); Trojanowski and Raizen (2016); Miyazaki, Liu and Hayashi ( 2017); others: Micchelli and Perrimon (2006), Ohlstein and Spradling (2006), Chaturvedi, Reichert, Gunage and VijayRaghavan ( 2017), Gunage, Dhanyasi, Reichert and VijayRaghavan (2017)…”

Making sense out of missense mutations: Mechanistic dissection of Notch receptors through structure‐function studies inDrosophila

“…Furthermore, these alleles have allowed researchers to explore the post-developmental roles of Notch signaling in adult flies, revealing its roles in tissue homeostasis through regulation of stem cells (Chaturvedi, Reichert, Gunage, & VijayRaghavan, 2017;Micchelli & Perrimon, 2006;Ohlstein & Spradling, 2006;Song, Call, Kirilly, & Xie, 2007), stereotypic behavior including learning and memory (Presente, Boyles, Serway, Belle, & Andres, 2004), and longevity (Presente, Andres, & Nye, 2001). Our understanding of Notch signal regulation will likely advance if we can precisely understand how the N ts1 missense mutation in EGFr-32 causes these temperature sensitive phenotypes at the molecular level.…”

An abundant quiescent stem cell population inDrosophilaMalpighian tubules protects principal cells from kidney stones

“…Active stem cells such as intestinal stem cells and germline stem cells have been extensively studied in Drosophila. However, studies on Drosophila quiescent stem cells are just emerging (Boukhatmi and Bray, 2018;Chaturvedi et al, 2017;Otsuki and Brand, 2018).…”