Identification and Functional Characterization of Plant MiRNA Under Salt Stress Shed Light on Salinity Resistance Improvement Through MiRNA Manipulation in Crops

Xu, Tao; Zhang, Long; Yang, Zhiyong; Wei, Yiliang; Dong, Tingting

doi:10.3389/fpls.2021.665439

Cited by 18 publications

(13 citation statements)

References 144 publications

(98 reference statements)

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“…Many researchers have reported that microRNA mediated a significant role in enhancing stress response in plants ( Su et al, 2021 ; Xu et al, 2021 ). Thus, for an in-depth comprehension of miRNA-triggered post-transcriptional modulations of StGLPs , we identified miRNAs targeting StGLPs genes.…”

Section: Resultsmentioning

confidence: 99%

Genome-Wide Identification and Expression Profiling of Germin-Like Proteins Reveal Their Role in Regulating Abiotic Stress Response in Potato

et al. 2022

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Germin and germin-like proteins (GLPs) perform a significant role in plants against biotic and abiotic stress. To understand the role of GLPs in potato, a comprehensive genome-wide analysis was performed in the potato genome. This study identified a total of 70 StGLPs genes in the potato genome, distributed among 11 chromosomes. Phylogenetic analysis exhibited that StGLPs were categorized into six groups with high bootstrap values. StGLPs gene structure and motifs analysis showed a relatively well-maintained intron–exon and motif formation within the cognate group. Additionally, several cis-elements in the promoter regions of GLPs were hormones, and stress-responsive and different families of miRNAs target StGLPs. Gene duplication under selection pressure also exhibited positive and purifying selections in StGLPs. In our results, the StGLP5 gene showed the highest expression in response to salt stress among all expressed StGLPs. Totally 19 StGLPs genes were expressed in response to heat stress. Moreover, three genes, StGLP30, StGLP17, and StGLP14, exhibited a relatively higher expression level in the potato after heat treatment. In total, 22 genes expressed in response to abscisic acid (ABA) treatment indicated that ABA performed an essential role in the plant defense or tolerance mechanism to environmental stress. RNA-Seq data validated by RT-qPCR also confirm that the StGLP5 gene showed maximum expression among selected genes under salt stress. Concisely, our results provide a platform for further functional exploration of the StGLPs against salt and heat stress conditions.

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Section: Resultsmentioning

confidence: 99%

Genome-Wide Identification and Expression Profiling of Germin-Like Proteins Reveal Their Role in Regulating Abiotic Stress Response in Potato

et al. 2022

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“…MicroRNAs (miRNA) are a class of non-coding RNA that play critical roles in regulating the expression of genes at the post-transcriptional level, which is important for plant growth, development, and adaptation to various biotic and abiotic stresses, including salinity stress [ 71 ]. Our data also showed higher expression of a gene encoding serine-/arginine-rich splicing factor RS41-like isoform X2 (cluster-30086.82952) in T03R than T22R.…”

Section: Discussionmentioning

confidence: 99%

Deciphering Molecular Mechanisms Involved in Salinity Tolerance in Guar (Cyamopsis tetragonoloba (L.) Taub.) Using Transcriptome Analyses

Sandhu

Dueñas

Ferreira

et al. 2022

Plants

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Guar is a commercially important legume crop known for guar gum. Guar is tolerant to various abiotic stresses, but the mechanisms involved in its salinity tolerance are not well established. This study aimed to understand molecular mechanisms of salinity tolerance in guar. RNA sequencing (RNA-Seq) was employed to study the leaf and root transcriptomes of salt-tolerant (Matador) and salt-sensitive (PI 340261) guar genotypes under control and salinity. Our analyses identified a total of 296,114 unigenes assembled from 527 million clean reads. Transcriptome analysis revealed that the gene expression differences were more pronounced between salinity treatments than between genotypes. Differentially expressed genes associated with stress-signaling pathways, transporters, chromatin remodeling, microRNA biogenesis, and translational machinery play critical roles in guar salinity tolerance. Genes associated with several transporter families that were differentially expressed during salinity included ABC, MFS, GPH, and P-ATPase. Furthermore, genes encoding transcription factors/regulators belonging to several families, including SNF2, C2H2, bHLH, C3H, and MYB were differentially expressed in response to salinity. This study revealed the importance of various biological pathways during salinity stress and identified several candidate genes that may be used to develop salt-tolerant guar genotypes that might be suitable for cultivation in marginal soils with moderate to high salinity or using degraded water.

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“…Constitutive expression of rice microRNA528 enhances tolerance to salinity stress and nitrogen starvation in creeping bentgrass ( Yuan et al, 2015 ). These results suggested that microRNAs could be used as a powerful tool for improving salinity resistance in crops ( Xu et al, 2021 ).…”

Section: Introductionmentioning

confidence: 97%

“…At present, miRNAs and their target genes related to salt stress have been identified in many plants, such as broccoli ( Brassica oleracea ), Populus tomentosa , radish ( Raphanus sativus ) and maize ( Zea mays ) ( Dong et al, 2009 ; Ren et al, 2013 ; Tian et al, 2014 ). MiRNA enables plant to adapt to salt by regulating salt stress-related genes ( Xu et al, 2021 ). Overexpression of miR156a decreased the salt tolerance of apple plant, while overexpression its target gene MdSPL13 improved salt tolerance ( Ma et al, 2020 ).…”

Section: Introductionmentioning

confidence: 99%

Comparative Analysis of Salt Responsive MicroRNAs in Two Sweetpotato [Ipomoea batatas (L.) Lam.] Cultivars With Different Salt Stress Resistance

et al. 2022

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Sweetpotato [Ipomoea batatas (L.) Lam.] is an important food, vegetable and economic crop, but its productivity is remarkably affected by soil salinity. MiRNAs are a class of endogenous non-coding small RNAs that play an important role in plant resistance to salt stress. However, the function of miRNAs still remains largely unknown in sweetpotato under salt stress. Previously, we identified salt-responsive miRNAs in one salt-sensitive sweetpotato cultivar “Xushu 32.” In this study, we identified miRNAs in another salt-tolerant cultivar “Xushu 22” by high-throughput deep sequencing and compared the salt-responsive miRNAs between these two cultivars with different salt sensitivity. We identified 687 miRNAs in “Xushu 22,” including 514 known miRNAs and 173 novel miRNAs. Among the 759 miRNAs from the two cultivars, 72 and 109 miRNAs were specifically expressed in “Xushu 32” and “Xushu 22,” respectively, and 578 miRNAs were co-expressed. The comparison of “Xushu 32” and “Xushu 22” genotypes showed a total of 235 miRNAs with obvious differential expression and 177 salt-responsive miRNAs that were obviously differently expressed between “Xushu 32” and “Xushu 22” under salt stress. The target genes of the miRNAs were predicted and identified using the Target Finder tool and degradome sequencing. The results showed that most of the targets were transcription factors and proteins related to metabolism and stress response. Gene Ontology analysis revealed that these target genes are involved in key pathways related to salt stress response and secondary redox metabolism. The comparative analysis of salt-responsive miRNAs in sweetpotato cultivars with different salt sensitivity is helpful for understanding the regulatory pattern of miRNA in different sweetpotato genotypes and improving the agronomic traits of sweetpotato by miRNA manipulation in the future.

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Identification and Functional Characterization of Plant MiRNA Under Salt Stress Shed Light on Salinity Resistance Improvement Through MiRNA Manipulation in Crops

Cited by 18 publications

References 144 publications

Genome-Wide Identification and Expression Profiling of Germin-Like Proteins Reveal Their Role in Regulating Abiotic Stress Response in Potato

Genome-Wide Identification and Expression Profiling of Germin-Like Proteins Reveal Their Role in Regulating Abiotic Stress Response in Potato

Deciphering Molecular Mechanisms Involved in Salinity Tolerance in Guar (Cyamopsis tetragonoloba (L.) Taub.) Using Transcriptome Analyses

Comparative Analysis of Salt Responsive MicroRNAs in Two Sweetpotato [Ipomoea batatas (L.) Lam.] Cultivars With Different Salt Stress Resistance

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