Identification and functional analysis of a galactosyltransferase capable of cholesterol glycolipid formation in the Lyme disease spirochete Borrelia burgdorferi

Hove, Petronella R.; Magunda, Forgivemore; Marques, Maria Angela de Mello; Islam, M. Nurul; Harton, Marisa; Jackson, Mary; Belisle, John T.

doi:10.1371/journal.pone.0252214

Cited by 6 publications

(4 citation statements)

References 65 publications

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“…Additionally, glycans are found on lipids ( Hove et al, 2021 ) and, as recently detailed, even on cell-surface RNAs ( Flynn et al, 2021 ). The omnipresence of polysaccharides on fundamental building blocks demonstrates their necessity across all levels of biological systems.…”

Section: Introductionmentioning

confidence: 96%

The Role of Fucose-Containing Glycan Motifs Across Taxonomic Kingdoms

Thomès

Bojar

2021

Front. Mol. Biosci.

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The extraordinary diversity of glycans leads to large differences in the glycomes of different kingdoms of life. Yet, while most monosaccharides are solely found in certain taxonomic groups, there is a small set of monosaccharides with widespread distribution across nearly all domains of life. These general monosaccharides are particularly relevant for glycan motifs, as they can readily be used by commensals and pathogens to mimic host glycans or hijack existing glycan recognition systems. Among these, the monosaccharide fucose is especially interesting, as it frequently presents itself as a terminal monosaccharide, primed for interaction with proteins. Here, we analyze fucose-containing glycan motifs across all taxonomic kingdoms. Using a hereby presented large species-specific glycan dataset and a plethora of methods for glycan-focused bioinformatics and machine learning, we identify characteristic as well as shared fucose-containing glycan motifs for various taxonomic groups, demonstrating clear differences in fucose usage. Even within domains, fucose is used differentially based on an organism’s physiology and habitat. We particularly highlight differences in fucose-containing motifs between vertebrates and invertebrates. With the example of pathogenic and non-pathogenic Escherichia coli strains, we also demonstrate the importance of fucose-containing motifs in molecular mimicry and thereby pathogenic potential. We envision that this study will shed light on an important class of glycan motifs, with potential new insights into the role of fucosylated glycans in symbiosis, pathogenicity, and immunity.

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Section: Introductionmentioning

confidence: 96%

The Role of Fucose-Containing Glycan Motifs Across Taxonomic Kingdoms

Thomès

Bojar

2021

Front. Mol. Biosci.

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“…Cholesteryl-acylphosphoglycosides (CPGs) have been detected in H. pylori [ 93 , 94 ] and Borella burgdorferi [ 95 , 96 ]. We confirm that H. pylori has these unique lipids and report for the first time that P. mirabilis also has these membrane lipids ( Supplementary Table S2 ).…”

Section: Resultsmentioning

confidence: 99%

“…Corynebacterium glutamicum, C. striatum [18] TDM (Trehalose dimycolate) Corynebacterium glutamicum, C. striatum [18] Acyltrehalose (MAT) and Di-Rhodococcus ruber, M. tuberculosis [33,34] -Phospho-Glycoside (CPG) Helicobacter pylori, Borrelia burgdorferi [93][94][95][96] Cholesteryl Phosphoethanolamine-Glycoside (CEPG) Helicobacter pylori [94] Bacteriohopanetetrol cyclitol ethers (BHT-CE) Burkholderia spp., Methylobacterium organophilum, Chloracidobacteria spp.…”

Section: Lipid Class Bacterial Strainsmentioning

confidence: 99%

Construction of a Bacterial Lipidomics Analytical Platform: Pilot Validation with Bovine Paratuberculosis Serum

Wood

Erol

2023

Metabolites

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Lipidomics analyses of bacteria offer the potential to detect and monitor infections in a host since many bacterial lipids are not present in mammals. To evaluate this omics approach, we first built a database of bacterial lipids for representative Gram-positive and Gram-negative bacteria. Our lipidomics analysis of the reference bacteria involved high-resolution mass spectrometry and electrospray ionization with less than a 1.0 ppm mass error. The lipidomics profiles of bacterial cultures clearly distinguished between Gram-positive and Gram-negative bacteria. In the case of bovine paratuberculosis (PTB) serum, we monitored two unique bacterial lipids that we also monitored in Mycobacterium avian subspecies PTB. These were PDIM-B C82, a phthiodiolone dimycocerosate, and the trehalose monomycolate hTMM 28:1, constituents of the bacterial cell envelope in mycolic-containing bacteria. The next step will be to determine if lipidomics can detect subclinical PTB infections which can last 2-to-4 years in bovine PTB. Our data further suggest that it will be worthwhile to continue building our bacterial lipidomics database and investigate the further utility of this approach in other infections of veterinary and human clinical interest.

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“…The B. burgdorferi membrane includes lipoproteins and lipids, such as phosphatidylcholine (PC), phosphatidylglycerol (PG), and cholesterol (5)(6)(7)(8). Because of an evolutionarily reduced genome, B. burgdorferi lack the metabolic pathways to synthesize many required lipids and rely on scavenging lipids from the host environment or culture medium (9).…”

Section: Dependence Of Borrelia On Host Lipidsmentioning

confidence: 99%

Use of host lipids by the Lyme disease spirochete may lead to biomarkers

Arora¹,

Hart²,

Fikrig³

2022

Journal of Clinical Investigation

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Insights into the challenges of Lyme disease diagnosisLyme disease is the most common tickborne infection in North America (1). Two-tiered serologic testing, consisting of an ELISA followed by immunoblotting against Borrelia antigens, is routinely used to aid clinicians in the diagnosis of Lyme disease. The sensitivity rates, however, are not always ideal, particularly during early infection, when Borrelia burgdorferispecific antibodies have not yet developed (2, 3). In addition, the long-term presence of B. burgdorferi-specific antibodies after an initial infection can make serodiagnosis of a repeat infection difficult. In this issue of the JCI, Gwynne et al. demonstrate that mice developed antiphospholipid antibodies following B. burgdorferi infection (Figure 1 and ref. 4). Further, the authors found many of the same antibodies in the sera of patients with Lyme disease, mostly in early disease marked by erythema migrans. A further comparison with sera from patients with syphilis implied that some of these antiphospholipid antibodies were specific to B. burgdorferi infection. The findings suggest that antibodies against specific phospholipids could aid in the diagnosis of early or repeat infection by the Lyme disease spirochete.

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Identification and functional analysis of a galactosyltransferase capable of cholesterol glycolipid formation in the Lyme disease spirochete Borrelia burgdorferi

Cited by 6 publications

References 65 publications

The Role of Fucose-Containing Glycan Motifs Across Taxonomic Kingdoms

The Role of Fucose-Containing Glycan Motifs Across Taxonomic Kingdoms

Construction of a Bacterial Lipidomics Analytical Platform: Pilot Validation with Bovine Paratuberculosis Serum

Use of host lipids by the Lyme disease spirochete may lead to biomarkers

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