Identification and function of FAR protein family genes from a transcriptome analysis of <i>Aphelenchoides besseyi</i>

Wang, Dong-Wei; Xu, Chunling; Ding, S.; Huang, Xin; Cheng, Xi; Zhang, Chao; Chen, Chun; Xie, Hui

doi:10.1093/bioinformatics/bty209

Cited by 12 publications

(18 citation statements)

References 45 publications

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“…For example, Ace-far-1 mRNA from Ancylostoma ceylanicum ; and Ab-far-1 , Ab-far-2 , and Ab-far-7 mRNAs from A. besseyi were also localized in genital system of nematodes [15,21,27]. Mhi-far-1 mRNA from M. hispanica was localized in the subesophageal gland of second-stage juveniles [23], and Ha-far-1 mRNA from H. avenae and Ab-far-3 mRNA from A. besseyi were localized in intestines of nematodes [12,27]. More interestingly, Ab-far-3 and Ab-far-5 mRNAs were also present in the nerve ring of A. besseyi [27].…”

Section: Discussionmentioning

confidence: 99%

“…Mhi-far-1 mRNA from M. hispanica was localized in the subesophageal gland of second-stage juveniles [23], and Ha-far-1 mRNA from H. avenae and Ab-far-3 mRNA from A. besseyi were localized in intestines of nematodes [12,27]. More interestingly, Ab-far-3 and Ab-far-5 mRNAs were also present in the nerve ring of A. besseyi [27]. In the study, Ar-far-1 mRNA was mainly detected in the muscle layer of CFN, especially in the muscle layers near the end of the esophageal glands which were different from the reported far mRNA [12,13,15,21,22,25,26,27].…”

Section: Discussionmentioning

confidence: 99%

“…More interestingly, Ab-far-3 and Ab-far-5 mRNAs were also present in the nerve ring of A. besseyi [27]. In the study, Ar-far-1 mRNA was mainly detected in the muscle layer of CFN, especially in the muscle layers near the end of the esophageal glands which were different from the reported far mRNA [12,13,15,21,22,25,26,27]. Ar-far-1 mRNA was also localized in the intestine, female genital system, and egg of CFN.…”

Section: Discussionmentioning

confidence: 99%

“…Approximately 20 μg of gDNA was obtained from CFN and digested with restriction enzymes (Dra I and EcoR I) (Figure S4). The digested DNA products were separated by 0.8% ( w / v ) agarose gel electrophoresis and transferred to a Hybond N + membrane (Amersham-Biosciences, Little Chalfont Buckinghamshire, England) [27,40]. The membrane was hybridized for 18 h at 42 °C with the probe.…”

Section: Methodsmentioning

confidence: 99%

“…With the development of transcriptome sequencing technology, the nematode-specific FAR proteins were being extensively discovered and studied [8,9,10,12,13,14,15,16,17,18,19,20,21,22,23,24,25,26,27]. According to our data, at least seventy FAR proteins have been identified in thirty-nine species of nematodes [8,9,10,12,13,14,15,16,17,18,19,20,21,22,23,24,25,26,27]. In plant-parasitic nematodes, twenty-six FAR proteins from seventeen species have been identified, most of which are Tylenchida nematodes, while only two species, A. besseyi and Bursaphelenchus xylophilus, are Aphelenchida nematodes.…”

Section: Introductionmentioning

confidence: 99%

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Identification and Characterization of a Fatty Acid- and Retinoid-Binding Protein Gene (Ar-far-1) from the Chrysanthemum Foliar Nematode, Aphelenchoides ritzemabosi

Ding

Wang

et al. 2019

IJMS

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The chrysanthemum foliar nematode (CFN), Aphelenchoides ritzemabosi, is a migratory, plant-parasitic nematode that is widely distributed and infects the aboveground parts of many plants. The fatty acid- and retinoid-binding proteins (FAR) are nematode-specific proteins that are involved in the development, reproduction, and infection of nematodes and are secreted into the tissues to disrupt the plant defense reaction. In this study, we obtained the full-length sequence of the FAR gene (Ar-far-1) from CFN, which is 727 bp and includes a 546 bp ORF that encodes 181 amino acids. Ar-FAR-1 from CFN has the highest sequence similarity to Ab-FAR-1 from A. besseyi, and they are located within the same branch of the phylogenetic tree. Fluorescence-based ligand-binding analysis confirmed that recombinant Ar-FAR-1 was bound to fatty acids and retinol. Ar-far-1 mRNA was expressed in the muscle layer, intestine, female genital system, and egg of CFN, and more highly expressed in females than in males among the four developmental stages of CFN. We demonstrated that the reproduction number and infection capacity of CFN decreased significantly when Ar-far-1 was effectively silenced by in vitro RNAi. Ar-far-1 plays an important role in the development, reproduction, infectivity, and pathogenesis of CFN and may be used as an effective target gene for the control of CFN. The results provide meaningful data about the parasitic and pathogenic genes of CFN to study the interaction mechanism between plant-parasitic nematodes and hosts.

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