Identification and frequency of circulating <scp>CD</scp>4<sup>+</sup> T lymphocytes specific to <scp>B</scp>enzylpenicillin in healthy donors

Nhim, Cathy; Delluc, Stéphanie; Halgand, F.; Chaisemartin, Luc de; Weaver, Richard J.; Claude, Nancy; Joseph, Delphine; Maillère, Bernard; Pallardy, Marc

doi:10.1111/all.12173

Cited by 28 publications

(58 citation statements)

References 31 publications

(43 reference statements)

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“…BP‐HSA was produced as described previously . The presence of BP on HSA was confirmed using Western blots and mass spectrometry MALDI‐TOF analysis and is published in a companion work .…”

Section: Methodsmentioning

confidence: 99%

“…BP‐HSA and BP‐haptenated peptides‐specific CD4 + and CD4 + CD45RA + T‐cell lines were generated as previously described . CD4 + T cells were restimulated on days 7, 14, and 21, with fresh autologous DCs loaded with BP‐HSA .…”

Section: Methodsmentioning

confidence: 99%

“…There is in vitro evidence that T cells from allergic patients react with BP‐HSA biocojugates . Our group has recently shown the existence of naïve CD4 + T cells specific for BP‐HSA in healthy donors . Moreover, activation of T cell clones from allergic patients may occur selectively in response to free drug or to drug conjugates .…”

Section: Introductionmentioning

confidence: 99%

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Identification of T‐cell epitopes from benzylpenicillin conjugated to human serum albumin and implication in penicillin allergy

Azoury

Filì

Bechara

et al. 2018

Allergy

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Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

See 1 more Smart Citation

Identification of T‐cell epitopes from benzylpenicillin conjugated to human serum albumin and implication in penicillin allergy

Azoury

Filì

Bechara

et al. 2018

Allergy

Self Cite

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“…To address this issue, Nhim et al [33] evaluated the frequency of benylpenicillinspecific T cells in healthy individuals. They found drugresponsive CD4 + T cells in 9 out of 10 donors and in each case, the T cells originated in the naive compartment.…”

Section: Mechanistic Features Of Drug Hypersensitivity Reactionsmentioning

confidence: 99%

Are drug metabolites able to cause T-cell-mediated hypersensitivity reactions?

Sullivan

Gibson

Park

et al. 2014

Expert Opinion on Drug Metabolism & Toxicology

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The role of metabolism in pathogenesis of immunological drug reactions has only been studied with a small number of drugs where synthetic metabolites are available for functional studies. In each case, metabolite-responsive T cells have been detected. However, the field is skewed by the fact that most research is conducted using the parent compound in metabolically inert cell systems. We propose that research efforts are directed towards the synthesis of drug metabolites and/or drug-protein conjugates. Furthermore, analytical methods need to be developed to relate metabolite exposure to the T-cell response. For now, our understanding of the chemical basis of drug hypersensitivity is incomplete.

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“…From a general perspective, it is necessary that several elements concur for activation of the immune system: an antigen that is processed and presented by antigen presenting cells (APC) to T-cells via MHC molecules, a T cell repertoire able to recognize this antigen and co-stimulatory signals or a regulatory response [1]. The interactions of drugs with proteins are critical for the induction of hypersensitivity responses.…”

Section: Introductionmentioning

confidence: 99%

Adduct Formation and Context Factors in Drug Hypersensitivity: Insight from Proteomic Studies

González-Morena¹,

Montanez²,

Aldini³

et al. 2017

CPD

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Drug hypersensitivity reactions result from the activation of the immune system by drugs or their metabolites. The clinical presentations of drug hypersensitivity can range from relatively mild local manifestations to severe systemic syndromes that can be life-threatening. As in other allergic reactions, the causes are multifactorial as genetic, metabolic and concomitant factors may influence the occurrence of drug hypersensitivity. Formation of drug protein adducts is considered a key step in drug adverse reactions, and in particular in the immunological recognition in drug hypersensitivity reactions. Nevertheless, non-covalent interactions of drugs with receptors in immune cells or with MHC clefts and/or exposed peptides can also play an important role. In recent years, development of proteomic approaches has allowed the identification and characterization of the protein targets for modification by drugs in vivo and in vitro, the nature of peptides exposed on MHC molecules, the changes in protein levels induced by drug treatment, and the concomitant modifications induced by danger signals, thus providing insight into context factors. Nevertheless, given the complexity and multifactorial nature of drug hypersensitivity reactions, understanding the underlying mechanisms also requires the integration of knowledge from genomic, metabolomic and clinical studies.

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Identification and frequency of circulating CD4⁺ T lymphocytes specific to Benzylpenicillin in healthy donors

Abstract: This study showed for the first time the existence of naive CD4(+) T lymphocytes specific to benzylpenicillin in healthy donors.

Cited by 28 publications

References 31 publications

Identification of T‐cell epitopes from benzylpenicillin conjugated to human serum albumin and implication in penicillin allergy

Identification of T‐cell epitopes from benzylpenicillin conjugated to human serum albumin and implication in penicillin allergy

Are drug metabolites able to cause T-cell-mediated hypersensitivity reactions?

Adduct Formation and Context Factors in Drug Hypersensitivity: Insight from Proteomic Studies

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Identification and frequency of circulating CD4+ T lymphocytes specific to Benzylpenicillin in healthy donors

Abstract: This study showed for the first time the existence of naive CD4(+) T lymphocytes specific to benzylpenicillin in healthy donors.

Cited by 28 publications

References 31 publications

Identification of T‐cell epitopes from benzylpenicillin conjugated to human serum albumin and implication in penicillin allergy

Identification of T‐cell epitopes from benzylpenicillin conjugated to human serum albumin and implication in penicillin allergy

Are drug metabolites able to cause T-cell-mediated hypersensitivity reactions?

Adduct Formation and Context Factors in Drug Hypersensitivity: Insight from Proteomic Studies

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Product

Resources

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Identification and frequency of circulating CD4⁺ T lymphocytes specific to Benzylpenicillin in healthy donors