Identification and fine mapping of two blast resistance genes in rice cultivar 93-11

Lei, Cailin; Hao, Kun; Yang, Yayuan; Ma, Jing; Wang, Shuai; Wang, Jiulin; Cheng, Zhijun; Zhao, Shasha; Zhang, Xin; Guo, Xiuping; Wang, Chunming; Wan, Jianmin

doi:10.1016/j.cj.2013.07.007

Cited by 35 publications

(37 citation statements)

References 64 publications

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“…Genetic studies on blast resistance initiated as early as 1960s and now more than 70 R genes have been identified in rice (Lei et al 2013) and majority of them have been identified from indica land races. Pib, Pita, Pi9, Piz-t and Pi2, Pid2, Pi36, Pi37, Pikm, Pi5, Pid3/Pi25, Pit, Pish, pi21, Pb1, Pia/PiCO39, Pi-kh/Pi54, Pik, Pik-p and Pi1 have been isolated and characterized (Hua et al 2012;Wang et al 1999;Bryan et al 2000;Qu et al 2006;Zhou et al 2006;Chen et al 2006Chen et al , 2011Liu et al 2007;Lin et al 2007;Ashikawa et al 2008;Lee et al 2009;Shang et al 2009;Hayashi 2009;Takahashi et al 2010;Fukuoka et al 2009;Hayashi et al 2010;Okuyama et al 2011;Cesari et al 2013;Rai et al 2011;Zhai et al 2011;Yuan et al 2011).…”

Section: Significance Of Rice Germplasmmentioning

confidence: 99%

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Molecular breeding of rice for improved disease resistance, a review

Khan

2015

Australasian Plant Pathol.

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Diseases are considered to be the major limiting factors in rice production around the globe. Considering rice as an important cereal crop, developing disease resistant cultivars is a prime objective of breeders. Compared to conventional breeding, molecular breeding especially using marker assisted selection appears to be more effective and precise. The best management of disease is to bring durable wide spectrum resistance in rice cultivars. This can be accomplished by accumulating both qualitative and quantitative resistance genes in to rice cultivars. Introducing these resistance genes from the wild relatives of rice in to commercial cultivars has greatly helped the breeders to accomplish this task. Marker assisted selection is extremely valuable in resolving the issues the breeders face with traditional breeding. A number of transgenic rice lines harboring the so called 'foreign' resistant genes have been produced. However such promising lines must be extensively replicated in fields to evaluate stable integration and continued expression of genes. None the less identification of disease resistance genes and quantitative trait loci (QTLs), use of marker assisted selection along with gene pyramiding and transgenic approaches all provide breeders a hope to build high yielding disease resistant cultivars. Based on such premises, we can truly envision broadening of our understanding the genetic and molecular basis of disease resistance in rice.

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Section: Significance Of Rice Germplasmmentioning

confidence: 99%

“…Recently Lei et al (2013) identified and mapped two blast resiatance genes Pi60(t) and Pi61(t) in cultivar 93-11 using F2 and F3 segregating generations.…”

Section: Significance Of Rice Germplasmmentioning

confidence: 99%

Molecular breeding of rice for improved disease resistance, a review

Khan

2015

Australasian Plant Pathol.

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“…Because these markers had high selection accuracy for resistant plant sources, they were suggested to be used in MAS for the resistant gene. Lei et al (2013) identified and mapped two blast resistance genes, Pi60(t) and Pi61(t), in Chinese rice cultivar 93-11 using F2 and F3 populations derived from a cross between the susceptible cv. Lijiangxintuanheigu and resistant cv.…”

Section: Blastmentioning

confidence: 99%

Current Applicable DNA Markers for Marker Assisted Breeding in Abiotic and Biotic Stress Tolerance in Rice (Oryza sativa L.)

Nogoy¹,

Song²,

Ouk³

et al. 2016

Plant Breed. Biotech.

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This is an Open-Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. ABSTRACT Abiotic and biotic stresses adversely affect rice (Oryza sativa L.) growth and yield. Conventional breeding is a very effective method to develop tolerant rice variety; however, it takes a decade long to establish a new rice variety. DNA-based markers have a huge potential to improve the efficiency and precision of conventional plant breeding via marker-assisted selection (MAS). The large number of quantitative trait loci (QTLs) mapping studies for rice has provided an abundance of DNA marker-trait associations. The limitations of conventional breeding such as linkage drag and lengthy time consumption can be overcome by utilizing DNA markers in plant breeding. The major applications of DNA markers such as MAS, QTL mapping and gene pyramiding have been surveyed. In this review, we presented the latest markers available for some of the most important abiotic and biotic stresses in rice breeding programs. Achieving a significant impact on crop improvement by marker assisted breeding (MAB) represents the great challenge for agricultural scientists in the next few decades.

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“…Moderate resistance of Nipponbare to M. oryzae race PO6-6 has been reported (23). Rice 93-11 is resistant to the major Chinese M. oryzae isolates and contains three blast R genes: Pi41, Pi60(t), and Pi61(t) (17,30).Four major QTLs for resistance to six isolates of M. oryzae were reported in a recombinant inbred line (RIL) population, containing 259 F 7 RILs, which was developed from a cross of Nipponbare and 93-11 (29). Three of these genes-qPi93-1, qPi93-2, and qPi93-3-originated from the resistant donor 93-11, and qPi93-3 was linked to SSR marker RM7102 on chromosome 12.…”

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Characterization of Rice Blast Resistance Gene Pi61(t) in Rice Germplasm

Ma¹,

Jia

2014

Plant Disease

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Ma, J., Jia, M. H., and Jia, Y. 2014. Characterization of rice blast resistance gene Pi61(t) in rice germplasm. Plant Dis. 98:1200-1204.Identification of resistance (R) genes to races of Magnaporthe oryzae in rice (Oryza sativa) germplasm is essential for the development of rice cultivars with long-lasting blast resistance. In the present study, one major quantitative trait locus, qPi93-3, was fine mapped using a recombinant inbred line (RIL), F 8 RIL171, derived from the cross between 'Nipponbare' and '93-11'. RIL171 contained a heterozygous qPi93-3 allele which was found to be resistant against nine U.S. common races-ID1, IA1, IB49, IE1, IA45, IB1, IC17, IB45, and IH1-of M. oryzae. An F 2 mapping population consisting of 2,381 individuals derived from RIL171was evaluated with a field isolate (race) ARB82 (IA1) of M. oryzae under greenhouse conditions. Disease reaction of a resistant/susceptible ratio of 3:1 was identified with F 2 :F 3 families. In total, 12 simple sequence repeat markers spanning qPi93-3 were used for fine mapping. Consequently, qPi93-3 was delimited to 4.2 Mb between RM3246 and RM7102. Three insertion-deletion (InDel) markers located between RM3246 and RM7102, that had previously used to map Pi61(t), showed that qPi93-3 was Pi61(t). The existence of Pi61(t) in 136 rice germplasm lines from the United States Department of Agriculture rice core collection was evaluated using Pi61(t)-specific InDel markers. Pi61(t) was identified as a source of resistance in 5 of the 136 lines. The characterized germplasm will be useful for rice breeders to use for improving blast resistance.Rice blast, caused by the filamentous fungal pathogen Magnaporthe oryzae, is one of the most devastating diseases that reduces rice yield around the world. Reliance on host resistance (R) is the most economical and environmentally friendly strategy to control rice blast. After the first blast R gene, Pia, was identified in 1967 (14), about 100 blast R genes have been mapped on rice chromosomes (1,22). Blast R genes were found in all chromosomes except chromosome 3, and some of them are clustered (i.e., chromosomes 6, 11, and 12) (1,18,22). However, major R genes are often rapidly overcome by new virulent M. oryzae isolates. Efforts have been made worldwide to identify a broad spectrum of resistance to blast (3,18).In many crops, the majority of traits are regulated by multiple genes rather than a single gene. These multiple genes, each with a relatively minor effect, are referred to as quantitative trait loci (QTLs; 4). Identification and mapping QTLs is an important strategy to discover blast R genes. Up to now, about 350 QTLs for blast resistance have been identified, and a few have been validated (1). In addition, 23 blast resistance genes have been mapped on rice chromosomes within identified QTL regions (22). DNA markers tightly linked to known R genes are vital for breeding via a marker-assisted selection (MAS) approach. Pik-m and Pik were differentiated by allele-specific DNA markers (2), while Pi-z and Pi-b were iden...

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Identification and fine mapping of two blast resistance genes in rice cultivar 93-11

Cited by 35 publications

References 64 publications

Molecular breeding of rice for improved disease resistance, a review

Molecular breeding of rice for improved disease resistance, a review

Current Applicable DNA Markers for Marker Assisted Breeding in Abiotic and Biotic Stress Tolerance in Rice (Oryza sativa L.)

Characterization of Rice Blast Resistance Gene Pi61(t) in Rice Germplasm

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