Identification and feeding characteristics of the mixotrophic flagellate Poterioochromonas malhamensis, a microalgal predator isolated from outdoor massive Chlorella culture

Ma, Mingyang; Gong, Yingchun

doi:10.1016/j.algal.2017.11.024

Cited by 54 publications

(37 citation statements)

References 38 publications

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“…The predator P. malhamensis was originally isolated from a crashed Chlorella culture and was identified based on morphological characteristics and molecular analysis (Ma et al. ). P. malhamensis was grown mixotrophically with a “wheat medium” (wheat:distilled water ratio of 10 g:1 liter; wheat sterilized with ultraviolet radiation for about 30 min).…”

Section: Methodsmentioning

confidence: 99%

“…Recently, it was reported that the grazing of P. malhamensis might pose the greatest contamination threat for mass algal cultures of Chlorella (Ma et al. ; Wang et al. , ), which is recognized as one of the most successful commercial microalgae because of its high growth rate and high lipid content (Milano et al.…”

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confidence: 99%

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confidence: 99%

“…Poterioochromonas malhamensis is a well-known and widespread mixotrophic flagellate, capable of both photosynthesis and phagotrophy that is now recognized as playing an important role in grazing bacteria and in regenerating nutrients within marine and freshwater planktonic ecosystems (Sanders et al 1990;Zhang and Watanabe 2001), and is also used as a model organism to study the mechanism of trophic transformation (Beisser et al 2017). Recently, it was reported that the grazing of P. malhamensis might pose the greatest contamination threat for mass algal cultures of Chlorella (Ma et al 2018;Wang et al 2017Wang et al , 2018, which is recognized as one of the most successful commercial microalgae because of its high growth rate and high lipid content (Milano et al 2016;Ndimba et al 2013).…”

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confidence: 99%

“…Previous studies on the feeding characteristics of P. malhamensis have shown that it has a large spectrum of food items, including Chlorella sp., Scenedesmus sp., Nanochloropsis oceanica, and Synechocystis sp., and that its grazing ability varies with different prey (microalgae) (Ma et al 2018;Touloupakis et al 2016;Zhang et al 2009). However, in most studies of zooplankton grazing selectivity, the prey are very diverse, with relatively fewer studies having focused on the effect of interspecific difference.…”

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Factors Affecting the Mixotrophic Flagellate Poterioochromonas malhamensis Grazing on Chlorella Cells

Wei

Wang

et al. 2019

J Eukaryotic Microbiology

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Grazing behaviour between protozoa and phytoplankton exists widely in planktonic ecosystems. Poterioochromonas malhamensis is a well‐known and widespread mixotrophic flagellate, which is recognized to play an important role within marine and freshwater planktonic ecosystems and regarded as the greatest contamination threat for mass algal cultures of Chlorella. In this study, a comprehensive range of factors, including morphological characters, biochemical compositions, and specific growth rate of ten species or strains of Chlorella, were evaluated for their effect on the feeding ability of P. malhamensis, which was assessed by two parameters: the clearance rate of P. malhamensis on Chlorella spp. and the specific growth rate of P. malhamensis. The results showed that the clearance rate of P. malhamensis was negatively correlated with cell wall thickness and specific growth rate of Chlorella spp., while the specific growth rate of P. malhamensis was positively correlated with carbohydrate percentage and C/N ratio and negatively correlated with protein, lipid percentage, and nitrogen mass. In conclusion, the factors influencing feeding selectivity include not only the morphological character and chemical composition of Chlorella, but also its population dynamics. Our study provides useful insights into the key factors that affect the feeding selectivity of P. malhamensis and provides basic and constructive data to help in screening for grazing‐resistant microalgae.

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Section: Methodsmentioning

confidence: 99%

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confidence: 99%

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confidence: 99%

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confidence: 99%

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Factors Affecting the Mixotrophic Flagellate Poterioochromonas malhamensis Grazing on Chlorella Cells

Wei

Wang

et al. 2019

J Eukaryotic Microbiology

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Growth of Photosynthetic Microorganisms in Different Photobioreactors Operated Outdoors

Touloupakis

Carlozzi

2021

Cyanobacteria Biotechnology

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Predator dispersal influences predator distribution but not prey diversity in pitcher plant microbial metacommunities

Cuellar-Gempeler

terHorst

Mason

et al. 2023

Ecology

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The spatial distribution of predators can affect both the distribution and diversity of their prey. Therefore, differences in predator dispersal ability that affect their spatial distribution, could also affect prey communities. Here, we use the microbial communities within pitcher plant leaves as a model system to test the relationship between predator (protozoa) dispersal ability and distribution, and its consequences for prey (bacteria) diversity and composition. We hypothesized that limited predator dispersal results in clustered distributions and heterogeneous patches for prey species, whereas wide predator dispersal and distribution could homogenize prey metacommunities. We analyzed the distribution of two prominent bacterivore protozoans from a 2‐year survey of an intact field of Sarracenia purpurea pitcher plants, and found a clustered distribution of Tetrahymena and homogeneous distribution of Poterioochromonas. We manipulated the sources of protozoan colonists and recorded protozoan recruitment and bacterial diversity in target leaves in a field experiment. We found the large ciliate, Tetrahymena, was dispersal limited and occupied few leaves, whereas the small flagellate Poterioochromonas was widely dispersed. However, the bacterial communities these protozoans feed on was unaffected by clustering of Tetrahymena, but likely influenced by Poterioochromonas and other bacterivores dispersing in the field. We propose that bacterial communities in this system are structured by a combination of well dispersed bacterivores, bacterial dispersal, and bottom‐up mechanisms. Clustered predators could become strong drivers of prey communities if they were specialists or keystone predators, or if they exerted a dominant influence on other predators in top‐down controlled systems. Linking dispersal ability within trophic levels and its consequences for trophic dynamics can lead to a more robust perspective on trophic metacommunities.

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Identification and feeding characteristics of the mixotrophic flagellate Poterioochromonas malhamensis, a microalgal predator isolated from outdoor massive Chlorella culture

Cited by 54 publications

References 38 publications

Factors Affecting the Mixotrophic Flagellate Poterioochromonas malhamensis Grazing on Chlorella Cells

Factors Affecting the Mixotrophic Flagellate Poterioochromonas malhamensis Grazing on Chlorella Cells

Growth of Photosynthetic Microorganisms in Different Photobioreactors Operated Outdoors

Predator dispersal influences predator distribution but not prey diversity in pitcher plant microbial metacommunities

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