Identification and expression profiling of neuropeptides and neuropeptide receptor genes in Atrijuglans hetaohei

Li, Feifei; Zhao, Xing; Zhu, Shuying; Wang, Tao; Li, Tianfeng; Woolfley, Tracy; Tang, Guanghui

doi:10.1016/j.gene.2020.144605

Cited by 7 publications

(8 citation statements)

References 42 publications

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“…This is, however, not the same conclusion drawn based on morphology and comparisons of mitochondrial genomes of Lepidoptera (Wang et al, 2016). Based on the predicted AKHs of A. hetaohei as deduced from transcriptome screening from heads of the moth (Li et al, 2020), we agree that A. hetaohei should be placed into family Stathmopodidae (superfamily Gelechioidea). Manse-AKH and Piebr-AKH both first appear in the superfamily Papillionoidea and cannot be reconciled to such an early appearance as in the superfamily Yponomeutoidea.…”

Section: Akhs Of Lepidoptera Are (Almost) Exclusively Order-specificcontrasting

confidence: 59%

“…6. The one investigated species in the superfamily Gelechioidea, Atrijuglans hetaohei, produces Manse-AKH and Piebr-AKH (see Li et al, 2020). Apparently, there is still much controversy and discussion about the taxonomic status and the phylogenetic position of A. hetaohei: Chinese scholars have widely applied the proposal that A. hetaohei is a member of Heliodinidae (superfamily Yponomeutoidea) (see Wang et al, 2016).…”

Section: Akhs Of Lepidoptera Are (Almost) Exclusively Order-specificmentioning

confidence: 99%

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Unique Members of the Adipokinetic Hormone Family in Butterflies and Moths (Insecta, Lepidoptera)

2020

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Lepidoptera is amongst one of the four most speciose insect orders and ecologically very successful because of their ability to fly. Insect flight is always aerobic and exacts a high metabolic demand on the animal. A family of structurally related neuropeptides, generically referred to as adipokinetic hormones (AKHs), play a key role in triggering the release of readily utilizable fuel metabolites into the hemolymph from the storage forms in the fat body. We used mass spectrometry to elucidate AKH sequences from 34 species of Lepidoptera and searched the literature and publicly available databases to compile (in a phylogenetic context) a comprehensive list of all Lepidoptera sequences published/predicted from a total of 76 species. We then used the resulting set of 15 biochemically characterized AKHs in a physiological assay that measures lipid or carbohydrate mobilization in three different lepidopteran species to learn about the functional cross-activity (receptor-ligand interactions) amongst the different butterfly/moth families. Our results include novel peptide structures, demonstrate structural diversity, phylogenetic trends in peptide distribution and order-specificity of Lepidoptera AKHs. There is almost an equal occurrence of octa-, nona-, and decapeptides, with an unparalleled emphasis on nonapeptides than in any insect order. Primitive species make Peram-CAH-II, an octapeptide found also in other orders; the lepidopteran signature peptide is Manse-AKH. Not all of the 15 tested AKHs are active in Pieris brassicae; this provides insight into structure-activity specificity and could be useful for further investigations into possible biorational insecticide development.

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Section: Akhs Of Lepidoptera Are (Almost) Exclusively Order-specificcontrasting

confidence: 59%

Section: Akhs Of Lepidoptera Are (Almost) Exclusively Order-specificmentioning

confidence: 99%

Unique Members of the Adipokinetic Hormone Family in Butterflies and Moths (Insecta, Lepidoptera)

2020

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“…In recent years, the continuous innovation of next-generation sequencing technology and bioinformatics technology has greatly promoted the prediction and identification of insect neuropeptides and their receptors (6)(7)(8)(9). However, only Acyrthosiphon pisum has so far been comprehensively investigated for neuropeptides and their receptors in aphids (10)(11)(12).…”

Section: Introductionmentioning

confidence: 99%

Identification and Characterization of Neuropeptides and Their G Protein-Coupled Receptors (GPCRs) in the Cowpea Aphid Aphis craccivora

Xiao

Du²,

Jiang³

et al. 2020

Front. Endocrinol.

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Neuropeptides are the most abundant and diverse signal molecules in insects. They act as neurohormones and neuromodulators to regulate the physiology and behavior of insects. The majority of neuropeptides initiate downstream signaling pathways through binding to G protein-coupled receptors (GPCRs) on the cell surface. In this study, RNA-seq technology and bioinformatics were used to search for genes encoding neuropeptides and their GPCRs in the cowpea aphid Aphis craccivora. And the expression of these genes at different developmental stages of A. craccivora was analyzed by quantitative real-time PCR (qRT-PCR). A total of 40 candidate genes encoding neuropeptide precursors were identified from the transcriptome data, which is roughly equivalent to the number of neuropeptide genes that have been reported in other insects. On this basis, software analysis combined with homologous prediction estimated that there could be more than 60 mature neuropeptides with biological activity. In addition, 46 neuropeptide GPCRs were obtained, of which 40 belong to rhodopsin-like receptors (A-family GPCRs), including 21 families of neuropeptide receptors and 7 orphan receptors, and 6 belong to secretin-like receptors (B-family GPCRs), including receptors for diuretic hormone 31, diuretic hormone 44 and pigment-dispersing factor (PDF). Compared with holometabolous insects such as Drosophila melanogaster, the coding genes for sulfakinin, corazonin, arginine vasopressin-like peptide (AVLP), and trissin and the corresponding receptors were not found in A. craccivora. It is speculated that A. craccivora likely lacks the above neuropeptide signaling pathways, which is consistent with Acyrthosiphon pisum and that the loss of these pathways may be a common feature of aphids. In addition, expression profiling revealed neuropeptide genes and their GPCR genes that are differentially expressed at different developmental stages and in different wing morphs. This study will help to deepen our understanding of the neuropeptide signaling systems in aphids, thus laying the foundation for the development of new methods for aphid control targeting these signaling systems.

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“…proteins were previously known to be expressed in the salivary glands. Moreover, we identified 77% of the proteins described by Wasinpiyamongkol et al 2010 As a class of neuronal signal molecules, neuropeptides are produced by the neurosecretory cells, that are mainly located in the brain, suboesophageal ganglion, among others (Li et al, 2020). In insects, neuropeptides and their receptors play important physiological processes such as development, reproduction, behavior and feeding (Holt et al, 2002).…”

Section: -Identification Of Salivary Gland Proteins Neuropeptides Anmentioning

confidence: 78%

Comprehensive quantitative proteome analysis of Aedes aegypti identifies proteins and pathways involved in Wolbachia pipientis and Zika virus interference phenomenon

Martins

Ramos

Murillo

et al. 2020

Preprint

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Zika virus is a global public health emergency due to its association with microcephaly, Guillain-Barré syndrome, neuropathy, and myelitis in children and adults. A total of 87 countries have had evidence of autochthonous mosquito-borne transmission of Zika virus, distributed across four continents, and no antivirus therapy or vaccines are available. Therefore, several strategies have been developed to target the main mosquito vector, Aedes aegypti, to reduce the burden of different arboviruses. Among such strategies, the use of the maternally-inherited endosymbiont Wolbachia pipientis has been applied successfully to reduce virus susceptibility and decrease transmission. However, the mechanisms by which Wolbachia orchestrate resistance to ZIKV infection remain to be elucidated. In this study, we apply isobaric labeling quantitative mass spectrometry-based proteomics to quantify proteins and identify pathways altered during ZIKV infection; Wolbachia infection; co-infection with Wolbachia/ZIKV in the Ae. aegypti heads and salivary glands. We show that Wolbachia regulates proteins involved in ROS production, regulates humoral immune response, and antioxidant production. The reduction of ZIKV polyprotein in the presence of Wolbachia in mosquitoes was determined by mass spectrometry and corroborates the idea that Wolbachia helps to block ZIKV infections in Ae. aegypti. The present study offers a rich resource of data that may help to elucidate mechanisms by which Wolbachia orchestrate resistance to ZIKV infection in Ae. aegypti, and represents a step further on the development of new targeted methods to detect and quantify ZIKV and Wolbachia directly in complex tissues.HighlightsThe abundance of ZIKV polyprotein is reduced in the presence of WolbachiaShotgun proteomics quantifies ZIKV and Wolbachia proteins directly in tissuesWolbachia regulates proteins involved in ROS productionWolbachia regulates humoral immune response and antioxidant productionMetabolism and detoxification processes were associated with mono infections

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Identification and expression profiling of neuropeptides and neuropeptide receptor genes in Atrijuglans hetaohei

Cited by 7 publications

References 42 publications

Unique Members of the Adipokinetic Hormone Family in Butterflies and Moths (Insecta, Lepidoptera)

Unique Members of the Adipokinetic Hormone Family in Butterflies and Moths (Insecta, Lepidoptera)

Identification and Characterization of Neuropeptides and Their G Protein-Coupled Receptors (GPCRs) in the Cowpea Aphid Aphis craccivora

Comprehensive quantitative proteome analysis of Aedes aegypti identifies proteins and pathways involved in Wolbachia pipientis and Zika virus interference phenomenon

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