Identification and expression profiling of putative chemosensory protein genes in two rice planthoppers, Laodelphax striatellus (Fallén) and Sogatella furcifera (Horváth)

Zhou, Wenwu; Yuan, Xin; Qian, Ping; Cheng, Jiaan; Zhang, Chuan‐Xi; Gurr, Geoff M.; Zhu, Zhiliang

doi:10.1016/j.aspen.2015.09.006

Cited by 22 publications

(19 citation statements)

References 34 publications

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“…For the first time, we had identified nine OBPs and nine CSPs , both from the M. persicae transcriptomic and genomic data, including four OBPs and five CSPs reported from the expressed sequence tags in M. persicae (Xu et al ., ). The number of M. persicae OBPs and CSPs identified here is less than the number of OBPs and CSPs identified in A. pisum (15 OBPs , 13 CSPs ) (Zhou et al ., ), the same as in A. gossypii (nine OBPs , nine CSPs ) (Gu et al ., ), and similar to those of other sucking insects such as the plant hoppers N. lugens (10 OBPs , 11 CSPs ) (Zhou et al ., ), S. furcifera (12 OBPs , nine CSPs ) (He and He, ; Zhou et al ., ) and the plant bug A. suturalis (16 OBPs , eight CSPs ) (Cui et al ., ). The high conservation in the sequence identities and genomic structures of the OBP and CSP orthologues among aphid species indicates that aphid OBPs and CSPs are conserved in a single copy across all aphids (with occasional losses), indicating that each OBP and CSP class evolved from a single gene in the common ancestor of aphids without subsequent duplication.…”

Section: Discussionmentioning

confidence: 99%

“…The protein names and sequences of the 199 OBPs and 103 CSPs from 18 different Hemiptera species are listed in Supporting Information Table S3. [Colour figure can be viewed at wileyonlinelibrary.com] the plant hoppers N. lugens (10 OBPs, 11 CSPs) (Zhou et al, 2014), S. furcifera (12 OBPs, nine CSPs) (He and He, 2014;Zhou et al, 2015) and the plant bug A. suturalis (16 OBPs, eight CSPs) (Cui et al, 2017). The high conservation in the sequence identities and genomic structures of the OBP and CSP orthologues among aphid species indicates that aphid OBPs and CSPs are conserved in a single copy across all aphids (with occasional losses), indicating that each OBP and CSP class evolved from a single gene in the common ancestor of aphids without subsequent duplication.…”

Section: Discussionmentioning

confidence: 99%

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Integrative transcriptomic and genomic analysis of odorant binding proteins and chemosensory proteins in aphids

Wang

Zhou

Liu

et al. 2018

Insect Molecular Biology

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Odorant binding proteins (OBPs) and chemosensory proteins (CSPs) play essential roles in insect chemosensory recognition. Here, we identified nine OBPs and nine CSPs from the Myzus persicae transcriptome and genome. Genomic structure analysis showed that the number and length of the introns are much higher, and this appears to be a unique feature of aphid OBP genes. Three M. persicae OBP genes (OBP3/7/8) as well as CSP1/4/6, CSP2/9 and CSP5/8 are tandem arrayed in the genome. Phylogenetic analyses of five different aphid species suggest that aphid OBPs and CSPs are conserved in single copy across all aphids (with occasional losses), indicating that each OBP and CSP class evolved from a single gene in the common ancestor of aphids without subsequent duplication. Motif pattern analysis revealed that aphid OBP and CSP motifs are highly conserved, and this could suggest the conserved functions of aphid OBPs and CSPs. Three OBPs (MperOBP6/7/10) are expressed antennae specifically, and five OBPs (MperOBP2/4/5/8/9) are expressed antennae enriched, consistent with their putative olfactory roles. M. persicae CSPs showed much broader expression profiles in nonsensory organs than OBPs. None of the nine MperCSPs were found to be antennae specific, but five of them (MperCSP1/2/4/5/6) showed higher expression levels in the legs than in other tissues. MperCSP10 mainly expressed in the antennae and legs. The broad and diverse expression patterns of M. persicae CSPs suggest their multifunctions in olfactory perception, development and other processes.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Integrative transcriptomic and genomic analysis of odorant binding proteins and chemosensory proteins in aphids

Wang

Zhou

Liu

et al. 2018

Insect Molecular Biology

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“…To assess the relationship of the Lygus CSPs with other insects, we constructed a maximum likelihood tree incorporating the complete repertoire of CSPs from species representing four additional insect orders: Diptera ( D. melanogaster ), Coleoptera ( Tribolium castaneum ), Hymenoptera ( Apis mellifera ), and Lepidoptera ( B. mori ). Similar to other reports (Vieira & Rozas, ; Kulmuni & Havukainen, ; Pelosi et al ., ; Zhou et al ., ), we found poor bootstrap support for deeper branches. Most CSPs grouped according to order and/or species with clear indications of gene expansion in T. castaneum and B. mori (Fig.…”

Section: Resultsmentioning

confidence: 57%

Molecular cloning and comparative analysis of transcripts encoding chemosensory proteins from two plant bugs, Lygus lineolaris and Lygus hesperus

2019

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Chemosensory proteins (CSPs) are soluble carrier proteins typically characterized by a six-helix bundle structure joined by two disulfide bridges and a conserved Cys spacing pattern (C1-X 6-8 -C2-X 16-21 -C3-X 2 -C4). CSPs are functionally diverse with reported roles in chemosensation, immunity, development, and resistance. To expand our molecular understanding of CSP function in plant bugs, we used recently developed transcriptomic resources for Lygus lineolaris and Lygus hesperus to identify 17 and 14 CSP-like sequences, respectively. The Lygus CSPs are orthologous and share significant sequence identity with previously annotated CSPs. Three of the CSPs are predicted to deviate from the typical CSP structure with either five or seven helical segments rather than six. The seven helix CSP is further differentiated by an atypical C3-X 3 -C4 Cys spacing motif. Reverse transcriptase PCR-based profiling of CSP transcript abundance in adult L. lineolaris tissues revealed broad expression for most of the CSPs with antenna specific expression limited to a subset of the CSPs. Comparative sequence analyses and homology modeling suggest that variations in the amino acids that comprise the Lygus CSP binding pockets affect the size and nature of the ligands accommodated.

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“…We first used the algorithm B asic L ocal A lignment S earch T ool (BLAST) to search the sequences of 10 known sphingolipid enzyme genes of insects or mammals. Then these known sphingolipid genes in GenBank were used to search for their homologous genes in the L. striatellus transcriptome database that was built in our previous work ( Zhou et al 2015 ). To confirm the identity of the putative sphingolipid genes in L. striatellus , BLASTX was performed to compare them against the non-redundant database at NCBI ( Table 1 ).…”

Section: Methodsmentioning

confidence: 99%

Rice Stripe Virus Infection Alters mRNA Levels of Sphingolipid-Metabolizing Enzymes and Sphingolipids Content inLaodelphax striatellus

Jiao

Bai

et al. 2017

J Insect Sci

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Sphingolipids and their metabolites have been implicated in viral infection and replication in mammal cells but how their metabolizing enzymes in the host are regulated by viruses remains largely unknown. Here we report the identification of 12 sphingolipid genes and their regulation by Rice stripe virus in the small brown planthopper (Laodelphax striatellus Fallén), a serious pest of rice throughout eastern Asia. According to protein sequence similarity, we identified 12 sphingolipid enzyme genes in L. striatellus. By comparing their mRNA levels in viruliferous versus nonviruliferous L. striatellus at different life stages by qPCR, we found that RSV infection upregulated six genes (LsCGT1, LsNAGA1, LsSGPP, LsSMPD4, LsSMS, and LsSPT) in most stages of L. striatellus. Especially, four genes (LsCGT1, LsSMPD2, LsNAGA1, and LsSMS) and another three genes (LsNAGA1, LsSGPP, and LsSMS) were significantly upregulated in viruliferous third-instar and fourth-instar nymphs, respectively. HPLC-MS/MS results showed that RSV infection increased the levels of various ceramides, such as Cer18:0, Cer20:0, and Cer22:0 species, in third and fourth instar L. striatellus nymphs. Together, these results demonstrate that RSV infection alters the transcript levels of various sphingolipid enzymes and the contents of sphingolipids in L. striatellus, indicating that sphingolipids may be important for RSV infection or replication in L. striatellus.

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Identification and expression profiling of putative chemosensory protein genes in two rice planthoppers, Laodelphax striatellus (Fallén) and Sogatella furcifera (Horváth)

Cited by 22 publications

References 34 publications

Integrative transcriptomic and genomic analysis of odorant binding proteins and chemosensory proteins in aphids

Integrative transcriptomic and genomic analysis of odorant binding proteins and chemosensory proteins in aphids

Molecular cloning and comparative analysis of transcripts encoding chemosensory proteins from two plant bugs, Lygus lineolaris and Lygus hesperus

Rice Stripe Virus Infection Alters mRNA Levels of Sphingolipid-Metabolizing Enzymes and Sphingolipids Content inLaodelphax striatellus

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