Identification and expression profiles of prdm1 in medaka Oryzias latipes

Zhao, Haobin; Zhang, Xiaosha; Cheng, Nana; Duan, Jundan; Wang, Jialin; Nagahama, Yoshitaka; Zhou, Qingchun; Wang, Yaping

doi:10.1007/s11033-013-2899-2

Cited by 16 publications

(17 citation statements)

References 60 publications

(122 reference statements)

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“…Previously, Prdm1a was reported involving in innate immunity in medaka and zebrafish (Zhao et al, 2014), and in the development and activation of B cells in zebrafish (Page et al, 2013). Bcl6aa A strong expression of bcl6aa was detected in the developing eyes of medaka embryos in 5 dpf suggested the major function of Bcl6aa in eye development.…”

Section: Discussionmentioning

confidence: 90%

“…In mammal, Bcl6 and Prdm1 are antagonists for each other to regulate immunity such as the development of B cells (Diehl et al, ) and T cells (Cimmino et al, ; Johnston et al, ). Previously, Prdm1a was reported involving in innate immunity in medaka and zebrafish (Zhao et al, ), and in the development and activation of B cells in zebrafish (Page et al, ). Bcl6aa or Bcl6ab functions possibly through repression of Prdm1a in the immune system of the teleost.…”

Section: Discussionmentioning

confidence: 99%

“…The cycling program was 95℃ 3 min; 30 cycles of 95℃ 30 s, 62℃ 30 s, 72℃ 25 s; and 72℃ 5 min. The 173 bp fragments of bcl6aa and the 195 bp fragments of bcl6ab were amplified with primers bcl6aa‐QF and bcl6aa‐QR, and bcl6ab‐QF and bcl6ab‐QR respectively, whereas the ribosomal protein RPS18 was used as internal control (Zhao et al, ) with primers RPS18QF and RPS18QR (Table ).…”

Section: Methodsmentioning

confidence: 99%

“…Adult fish were randomly divided into three groups and wereintraperitoneally injected with 10 μl of phosphate buffer solution (PBS), LPS (serotype: O55:B5; MilliporeSigma, St. Louis, MO), polyI:C (MilliporeSigma), respectively (Zhang et al, ; Zhao et al, ). LPS and polyI:C were dissolved in PBS in a concentration of 5 μg/μl, respectively.…”

Section: Methodsmentioning

confidence: 99%

“…The specific primers were bcl6aa-OF and bcl6aa-OR for bcl6aa and bcl6ab-OF and bcl6ab-OR for bcl6ab (Table 1). and the 195 bp fragments of bcl6ab were amplified with primers bcl6aa-QF and bcl6aa-QR, and bcl6ab-QF and bcl6ab-QR respectively, whereas the ribosomal protein RPS18 was used as internal control (Zhao et al, 2014) with primers RPS18QF and RPS18QR (Table 1).…”

Section: Identificationofbcl6aaandbcl6ab In Medakamentioning

confidence: 99%

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Bcl6aa and bcl6ab are ubiquitously expressed and are inducible by lipopolysaccharide and polyI:C in adult tissues of medaka Oryzias latipes

Zhang

et al. 2019

J Exp Zool Pt B

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B‐cell lymphoma‐6 (Bcl6) is a transcriptional repressor that plays important roles in various physiological activities such as innate and adaptive immune response, lymphocyte differentiation, and cell cycle regulation in mammals. Two homologs of Bcl6a, namely Bcl6aa and Bcl6ab, are identified in teleost fish including medaka Oryzias latipes. The expression profiles of bcl6aa and bcl6ab in medaka were studied using reverse‐transcription polymerase chain reaction and in situ hybridization. The transcripts of bcl6aa and bcl6ab were detected from very early embryos such as the four‐cell stage until hatching. Bcl6aa and bcl6ab were clearly detected in the embryonic body from 5 days postfertilization onward by in situ hybridization. Bcl6aa was specifically expressed in the retina, whereas bcl6ab was expressed in entire embryonic body. The results referred to that both bcl6aa and bcl6ab originate maternally in the zygotes and may play major roles in embryogenesis of medaka. The transcripts of bcl6aa and bcl6ab were detected in all examined adult tissues, including immune organs such as the gill, spleen, kidney, liver, and intestine. The expression of bcl6aa and bcl6ab in the liver, spleen, head–kidney, and intestine could be upregulated or downregulated by lipopolysaccharide and polyriboinosinic–polyribocytidylic acid. These results indicate that both bcl6aa and bcl6ab may be involved in immune response in medaka.

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Section: Discussionmentioning

confidence: 90%

Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Identificationofbcl6aaandbcl6ab In Medakamentioning

confidence: 99%

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Bcl6aa and bcl6ab are ubiquitously expressed and are inducible by lipopolysaccharide and polyI:C in adult tissues of medaka Oryzias latipes

Zhang

et al. 2019

J Exp Zool Pt B

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Identification, expression pattern, and immune response of Tim‐1 and Tim‐4 in embryos and adult medaka (Oryzias latipes)

Shen

Nibona

et al. 2020

J Exp Zool Pt B

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T‐cell immunoglobulin (Ig) and mucin domain‐containing 1 (Tim‐1) and Tim‐4 are two members of the Tim family. In mammals, Tim‐1 and Tim‐4 are proteins mainly expressed in immune cells and are associated with immune response. In the present study, medaka Oryzias latipes' Tim‐1 (OlTim‐1) and OlTim‐4 were identified and characterized using bioinformatics analyses. With the use of reverse‐transcription polymerase chain reaction, the expression profiles of OlTim‐1 and OlTim‐4 were examined in embryos and adult fish and in immune tissues following the intraperitoneal injection of stimulants. The results revealed that OlTim‐1 possesses a cytoplasmic region, a transmembrane region, a mucin domain, and an Ig‐like domain, while OlTim‐4 is composed of two Ig‐like domains and a mucin domain, but without the transmembrane region and cytoplasmic region. OlTim‐1 and OlTim‐4 expressions are detectable from the gastrula stage on, indicating that they are zygotic genes. Furthermore, OlTim‐1 and OlTim‐4 are expressed ubiquitously in the adult. Administration of immune stimulants, namely lipopolysaccharides and polyinosinic:polycytidylic acid, significantly increased the expression levels of OlTim‐1 and OlTim‐4 in the liver and intestine within 1 day and in the head, kidney, and spleen within 3 to 4 days postinjection. These results suggest that OlTim‐1 and OlTim‐4 are possibly involved in both innate and adaptive immunities.

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Sequences analyses and expression profiles in tissues and embryos of Japanese flounder (Paralichthys olivaceus) PRDM1

Liu

Lin

et al. 2015

Fish Physiol Biochem

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PRDM1 (PRDI-BF1-RIZ1 homologous domain containing 1) appears to be a pleiotropic regulatory factor in various processes. It contains a PR (PRDI-BF1-RIZ1 homologous) domain protein and five zinc fingers. In the present study, a gene coding the homolog of prdm1 and the 5' regulatory region of prdm1 was identified from the Paralichthys olivaceus (denoted Po-prdm1). Results of real-time quantitative polymerase chain reaction amplification (RT-qPCR) and in situ hybridization (ISH) in embryos revealed that Po-prdm1 was highly expressed between the early gastrula and tail bud stages, with its expression peaking in the mid-gastrula stage, whereas the results of RT-qPCR and ISH in tissues demonstrated that Po-prdm1 transcripts were ubiquitously detected in all tissues, which indicates its pleiotropic function in multiple processes. ISH of gonadal tissues revealed that the transcripts were located in the nucleus and cytoplasm of the oocytes in the ovaries but only in the spermatogonia and not in the spermatocytes in the testes. The Po-prdm1 transcription factor binding sites and their conserved binding region among vertebrates were analyzed in this study. The combined results suggest that Po-PRDM1 has a conserved function in teleosts and mammals.

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Identification and expression profiles of prdm1 in medaka Oryzias latipes

Cited by 16 publications

References 60 publications

Bcl6aa and bcl6ab are ubiquitously expressed and are inducible by lipopolysaccharide and polyI:C in adult tissues of medaka Oryzias latipes

Bcl6aa and bcl6ab are ubiquitously expressed and are inducible by lipopolysaccharide and polyI:C in adult tissues of medaka Oryzias latipes

Identification, expression pattern, and immune response of Tim‐1 and Tim‐4 in embryos and adult medaka (Oryzias latipes)

Sequences analyses and expression profiles in tissues and embryos of Japanese flounder (Paralichthys olivaceus) PRDM1

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