Identification and Expression Profile Analysis of Odorant Binding Proteins in the Oriental Fruit Fly Bactrocera dorsalis

Abstract

Olfaction is crucial in many insects for critical behaviors, including those regulating survival and reproduction. Insect odorant-binding proteins (OBPs) function in the first step of the olfactory system and play an essential role in the perception of odorants, such as pheromones and host chemicals. The oriental fruit fly, Bactrocera dorsalis, is a destructive fruit-eating pest, due to its wide host range of up to 250 different types of fruits and vegetables, and this fly causes severe economic damage to the fruit and vegetable industry. However, OBP genes have not been largely identified in B. dorsalis. Based on our previously constructed B. dorsalis cDNA library, ten OBP genes were identified in B. dorsalis for the first time. A phylogenetic tree was generated to show the relationships among the 10 OBPs of B. dorsalis to OBP sequences of two other Dipteran species, including Drosophila melanogaster and the mosquito Anopheles gambiae. The expression profiles of the ten OBPs in different tissues (heads, thoraxes, abdomens, legs, wings, male antennae and female antenna) of the mated adults were analyzed by real-time PCR. The results showed that nine of them are highly expressed in the antenna of both sexes, except BdorOBP7. Four OBPs (BdorOBP1, BdorOBP4, BdorOBP8, and BdorOBP10) are also enriched in the abdomen, and BdorOBP7 is specifically expressed in leg, indicating that it may function in other biological processes. This work will provide insight into the roles of OBPs in chemoreception and help develop new pest-control strategies.

Sniffing Out Chemosensory Genes from the Mediterranean Fruit Fly, Ceratitis capitata

“…Specifically, we considered: ten OBPs from B. dorsalis [47] and BdorOBP2 (unpublished, GenBank accession no. AGO28153), nine OBPs from R. suavis [48], and fifteen OBPs from R. pomonella [49].…”

Novel odorant-binding proteins and their expression patterns in grasshopper, Oedaleus asiaticus

“…Additionally, several OBPs are female-biased expression, such as OBP1, OBP2, OBP3, OBP4, OBP5, OBP8, OBP9, OBP10, and OBP13. These may be involved in female-specific chemosensory processes, such as laying eggs [20]. Among these OBPs, OBP5 is barely detected in male antennae and may be involved in recognition of male-specific pheromones.…”

Protein Baits, Volatile Compounds and Irradiation Influence the Expression Profiles of Odorantbinding Protein Genes in Bactrocera Dorsalis (Diptera: Tephritidae)

“…A phylogenetic tree was generated by obtaining the amino acid sequences of the ten putative OBPs of B. dorsalis from the work of (Zheng et al, 2013). The phylogenetic tree was built to demonstrate the relationships of the 10 BdorOBPs to 140 OBP sequences from 7 different orders and families of insects that possess the same OBP family, including 20 OBP sequences from Apis mellifera (Hymenoptera: Apidae), 7 OBP sequences from Bombyx mori (Lepidoptera: Bombycidae), 30 OBP sequences Idrees et (Figure 2).…”

“…The results of that study revealed that volatiles could potentially evoke higher relative EAG values in the antennae of B. dorsalis during three adult physiological stages (Jang and Light, 1991;Du et al, 2015). A total of 20 BdorOBP genes have been identified from the B. dorsalis transcriptome; however, only 10 BdorOBPs have been characterized (Zheng et al, 2012b(Zheng et al, , 2013.…”

“…A variety of methods such as the sterile insect technique, mass trapping, protein bait spraying and male annihilation have been developed to suppress or eradicate B. dorsalis and related species throughout Asia and the Pacific regions (Jessup et Chemoreception is an important characteristic in the selection of host plants by phytophagous insects. Odorant-binding proteins (OBPs) present in the olfactory system play an essential role in sensitivity to odorants, such as pheromones and host chemicals (Zheng et al, 2013). The olfactory system in insects consists of three central types of proteins: OBPs, odorant receptors (ORs) and odorant-degrading esterases (ODEs) (Justice et al, 2003).…”