Identification and expression of water stress- and abscisic acid-regulated genes in a drought-tolerant sunflower genotype

Ouvrard, Olivier; Cellier, Françoise; Ferrare, Karine; Tousch, Didier; Lamaze, Thierry; Dupuis, Jean‐Marc; Casse-Delbart, Francine

doi:10.1007/bf00019469

Cited by 133 publications

(81 citation statements)

References 48 publications

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“…Alignment of amino acid sequences was performed by using CLUSTALW (Des Higgins, European Bioinformatics Institute, U.K.) with manual correction of gaps, and an Elip consensus sequence was obtained by using the MULTALIN program (20). An Elip consensus sequence was constructed on the basis of Elip or Elip-related amino acid sequences in Pisum sativum (21), Hordeum vulgare (13), Glycine max (22), Helianthus annuus (23), and Craterostigma plantagineum (24), the red algae, such as Porphyra purpurea (25) and Cyanidium caldarium (26), the green alga Dunaliella bardawil (27), and the cyanobacteria Synechocystis (9).…”

Section: Methodsmentioning

confidence: 99%

Light stress-regulated two-helix proteins in Arabidopsis thaliana related to the chlorophyll a/b-binding gene family

Heddad¹

2000

Proceedings of the National Academy of Sciences

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The chlorophyll a͞b, chlorophyll a͞c, and chlorophyll a͞a light-harvesting proteins are part of an extended gene family that also includes the transiently expressed stress proteins, the Elips (early light-induced proteins). Four Elip homologue proteins, encoded by single-copy nuclear genes, have been identified in the Arabidopsis thaliana database. These proteins were divided into two groups according to the expression pattern under light-stress conditions and the predicted secondary structure. Group one included two members of the Elip family with three predicted transmembrane helices and a gene expression strictly related to light stress. Group two included two proteins, the Seps (stress-enhanced proteins), which possessed two predicted transmembrane segments. The transcripts of Sep1 and Sep2 were present under low light conditions, but their level increased 4-to 10-fold during illumination of plants with high-intensity light. Preliminary data indicated that the induced transcripts were translated in vivo. Other physiological stress conditions, such as cold, heat, desiccation, salt, wounding, or oxidative stress, did not significantly influence the expression of Sep genes. In vitro import of radioactively labeled precursors of Seps into isolated chloroplasts confirmed the thylakoid membrane localization of these proteins. Considering the predicted protein structure and homology to other pigment-antenna proteins, the two-helix Seps might represent an evolutionary missing link between the one-and three-helix antenna proteins present in pro-and eukaryota.ancestors ͉ chloroplast ͉ pigment proteins P hotosynthetic eukaryotes are traditionally divided into three major groups, largely on the basis of their light-harvesting antenna systems. The Chlorophytes (green algae and higher plants) have chlorophyll a͞b antennas, the Chromophytes have chlorophyll a͞c antennas, and the Rhodophytes (red algae) have only chlorophyll a and use phycobilisomes as the major photosystem (PS) II antenna (1, 2). The primary function of photosynthetic lightharvesting complexes is the absorption of light and the transfer of the excitation energy to the photochemical reaction centers. Because of their high sequence homology and similar structure and function, all of the eukaryotic light-harvesting antenna proteins are considered part of an extended gene family.The chlorophyll a͞b-binding (Cab) family in Arabidopsis thaliana contains at least 30 different members, associated with PSI or PSII (3). During the last few years, the Cab gene family was extended by the distant relatives, the PSII-S protein (4-6), the one-helix protein, the OHP (3), and the early light-induced proteins, the Elips (7, 8). The discovery of the cyanobacterial Hlip (high light-induced proteins) and the SCPs (small Cab-like proteins), clearly sharing the same conserved residues with the eukaryotic Cab proteins (9, 10), supported the idea that the prokaryotic Hlips and SCPs and the eukaryotic antenna proteins had a common ancestor (1, 11).The higher plant Elips are nuclear...

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Section: Methodsmentioning

confidence: 99%

Light stress-regulated two-helix proteins in Arabidopsis thaliana related to the chlorophyll a/b-binding gene family

Heddad¹

2000

Proceedings of the National Academy of Sciences

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“…Based on the presence of general and specific stress tolerance mechanisms (4), it is logical to expect plants to have multiple stress perception and signal transduction pathways, which may cross-talk at various steps. The alteration of protein synthesis or degradation is one of the fundamental metabolic processes that may influence dehydration tolerance (5,6). Both quantitative and qualitative changes of proteins have been detected during dehydration stress (7).…”

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confidence: 99%

Proteomics Approach to Identify Dehydration Responsive Nuclear Proteins from Chickpea (Cicer arietinum L.)

Pandey

Chakraborty

Datta

et al. 2008

Molecular & Cellular Proteomics

170

149

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Dehydration or water-deficit is one of the most important environmental stress factors that greatly influences plant growth and development and limits crop productivity. Plants respond and adapt to such stress by altering their cellular metabolism and activating various defense machineries. Mechanisms that operate signal perception, transduction, and downstream regulatory events provide valuable information about the underlying pathways involved in environmental stress responses. The nuclear proteins constitute a highly organized, complex network that plays diverse roles during cellular development and other physiological processes. To gain a better understanding of dehydration response in plants, we have developed a comparative nuclear proteome in a food legume, chickpea (Cicer arietinum L.). Three-week-old chickpea seedlings were subjected to progressive dehydration by withdrawing water and the changes in the nuclear proteome were examined using two-dimensional gel electrophoresis. Approximately 205 protein spots were found to be differentially regulated under dehydration. Mass spectrometry analysis allowed the identification of 147 differentially expressed proteins, presumably involved in a variety of functions including gene transcription and replication, molecular chaperones, cell signaling, and chromatin remodeling. The dehydration responsive nuclear proteome of chickpea revealed a coordinated response, which involves both the regulatory as well as the functional proteins. This study, for the first time, provides an insight into the complex metabolic network operating in the nucleus during dehydration. Molecular & Cellular Proteomics 7:88 -107, 2008.Environmental stress limits growth, development, and crop productivity (1, 2) and plays a major role in determining the geographic distribution of plant species. Several environmental stresses are united by the fact that at least part of their detrimental effect on plant performance is caused by disruption of plant water status. Periods of little or no rainfall can lead to a meteorological condition called drought. However, water deficit or dehydration, can also occur at conditions in which water is not limiting, through altered ion content and water uptake caused by high salinity or by formation of extracellular ice during freezing. Desiccation is the extreme form of dehydration wherein most of the protoplasmic "free" water is lost and the cell survival relies on the 'bound' water associated with the cell matrix.

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“…Linking the expression of a gene to a high degree of tolerance suggests a possible role for this gene in adaptation (Ouvrard et al 1996). It is thus critical to study the functions of stress-induced genes to understand the mechanisms involved in stress tolerance in plants.…”

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confidence: 99%

Differential expression of dehydrin genes in hull-less barley (Hordeum vulgare ssp. vulgare) depending on duration of dehydration stress

Qian¹,

Liu²,

Ao³

et al. 2008

Can. J. Plant Sci.

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M. 2008. Differential expression of dehydrin genes in hull-less barley (Hordeum vulgare ssp. vulgare) depending on duration of dehydration stress. Can. J. Plant Sci. 88: 899Á906. Dehydrins (DHN) are water-soluble lipid-associating proteins that accumulate during low-temperature and water-stress conditions, and are thought to play a protective role in freezing and drought tolerance in plants. The synthesis of the dehydrins, representing a link between environment and nuclear activity through dehydrin genes (Dhn) expression, was known to be a common response to drought in plants. The association of mRNA accumulations of Dhn genes (Dhn6, Dhn11 and Dhn13) with phenotypically diverse drought-tolerant genotypes was investigated in hull-less barley (Hordeum vulgare subsp. vulgare). Notably, differences between genotypes were observed, mainly in Dhn6 and Dhn13 gene, depending on the duration of the dehydration stress. Compared with drought-sensitive genotypes, the relative expression levels of Dhn6 gene were significantly higher in drought-tolerant lines subjected to 8 h water deficit. By control with continuative ascending accumulations in sensitive plants during drought, the highest transcript level of Dhn13 was observed in tolerant genotypes after 8 h of dehydration, and tended to descend the same levels as sensitive lines subjected to 12 h of dehydration. Based on decreased accumulations of Dhn11 during drought stress, it was concluded that Dhn11 was not up-regulated by water stress between genotypes. The observed differences in the present study may indicate an association of physiological response to water stress with differential accumulations of Dhn genes between drought-contrasting genotypes. The authors suggest different DHNs may play variable functional roles in structural protection in plants subjected to progressive water stress, associated with drought-contrasting genotypes.Key words: Dhydrin genes, quantitative reverse transcription polymerase chain reaction, water deficit, Tibetan hull-less barley Qian, G., Liu, Y., Ao, D., Yang, F. et Yu, M. 2008. Variation de l'expression des ge`nes de la de´hydrine dans une orge nue (Hordeum vulgare ssp. vulgare) selon la dure´e de la de´shydratation. Can. J. Plant Sci. 88: 899Á906. La de´hydrine (DHN) est une prote´ine lipophile hydrosoluble qui s'accumule quand la tempe´rature descend et qu'il y a stress hydrique. On pense qu'elle prote`ge la plante contre le gel et lui permet de tole´rer la se´cheresse. La synthe`se des de´hydrines, qui repre´sente un lien entre l'environnement et l'activite´du noyau par le biais des ge`nes qui la codent (Dhn), est une re´action commune a`la se´cheresse chez les ve´ge´taux. Les auteurs ont e´tudie´la relation entre l'accumulation de l'ARNm des ge`nes Dhn (Dhn6, Dhn11 et Dhn13) et des varie´te´s de phe´notype variable tole´rant la se´cheresse chez l'orge nue (Hordeum vulgare subsp. vulgare). Ils ont ainsi observe´des variations entre les ge´notypes, principalement au niveau des ge`nes Dhn6 et Dhn13, selon la dure´e du stress engend...

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Identification and expression of water stress- and abscisic acid-regulated genes in a drought-tolerant sunflower genotype

Cited by 133 publications

References 48 publications

Light stress-regulated two-helix proteins in Arabidopsis thaliana related to the chlorophyll a/b-binding gene family

Light stress-regulated two-helix proteins in Arabidopsis thaliana related to the chlorophyll a/b-binding gene family

Proteomics Approach to Identify Dehydration Responsive Nuclear Proteins from Chickpea (Cicer arietinum L.)

Differential expression of dehydrin genes in hull-less barley (Hordeum vulgare ssp. vulgare) depending on duration of dehydration stress

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