Identification and expression of TRAF6 (TNF receptor-associated factor 6) gene in Zhikong Scallop Chlamys farreri

Qiu, Limei; Song, Linsheng; Yu, Yundong; Zhao, Jianmin; Wang, Lingling; Zhang, Qing‐Chun

doi:10.1016/j.fsi.2008.10.010

Cited by 67 publications

(27 citation statements)

References 64 publications

(72 reference statements)

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“…Thus, TRAF6 is functionally important in innate immunity. The first experimentally identified molluscan TRAF6 was obtained from A. farreri (CfTRAF6) (Qiu et al, 2009). CfTRAF6 mRNA expression was greatly inhibited by peptidoglycan, indicating its function in the immune response of scallop.…”

Section: Discussionmentioning

confidence: 99%

“…Interestingly, similar to A. farreri (Qiu et al, 2009), additional amino acids were located between the second TRAF domain and the coiled-coil region was inserted in P. martensii, M. yessoensis, E. scolopes, and S. purpuratus. Further analysis of this insertion using the SMART software indicated that a low-complexity region (LCR) was contained in the TRAF6 proteins of mollusk, including P. martensii, A. farreri, and M. yessoensis (Figure 3).…”

Section: Homology Analysis Of Pmtraf6mentioning

confidence: 92%

“…In mammals, there are seven known TRAF family members (TRAF1-7) that play essential roles in signal transduction (Qiu et al, 2009;He et al, 2013). In both the gene structure and protein sequence homology of the C-terminal domain that is responsible for receptor binding, TRAF6 is the most divergent TRAF (Lomaga et al, 1999).…”

Section: Introductionmentioning

confidence: 99%

“…TRAF-6 may be a key molecule that participates in the innate defense system of mollusks (Qiu et al, 2009;He et al, 2013). The pearl oyster Pinctada martensii is the main species for marine pearl production in China and Japan.…”

Section: Introductionmentioning

confidence: 99%

“…The identification and characterization of TRAF6 in P. martensii will help our understanding of the innate immune response in pearl oyster. Although the TRAF6 gene has been cloned and identified in several marine invertebrates, such as Mizuhopecten yessoensis (He et al, 2013) and Azumapecten farreri (Qiu et al, 2009), the TRAF6 gene has not been characterized in detail and identified in P. martensii. To help our understanding of the innate immune response in pearl oyster, the aim of this research was to obtain the full-length TRAF6 gene and to investigate the TRAF6 gene expression patterns in different tissues and the temporal expression after LPS challenge in P. martensii.…”

Section: Introductionmentioning

confidence: 99%

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Molecular characterization of tumor necrosis factor receptor-associated factor 6 (TRAF6) in pearl oyster Pinctada martensii

Jiao¹,

Tian²,

Du³

et al. 2014

Genet. Mol. Res.

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ABSTRACT. Tumor necrosis factor receptor-associated factor 6 (TRAF6) is a key signaling adaptor molecule for tumor necrosis factor receptor superfamily and Toll-like receptor/interleukin-1 receptor family members. It signals the upstream receptors and is involved in a wide range of biological functions, such as immunity and bone metabolism. In this report, the TRAF6 gene from the pearl oyster Pinctada martensii (designated as PmTRAF6) was identified and characterized. The obtained full-length PmTRAF6 cDNA was 2273 bp, containing a 5'-untranslated region (UTR) of 297 bp, a 3'-UTR of 128 bp with a 42-bp poly (A) tail, and an open reading frame of 1848 bp that encoded 616-amino acid residues. The deduced protein sequence of PmTRAF6 contained a conserved TRAF family motif including a RING-type zinc finger, two TRAF-type zinc fingers, and a coiled-coil region followed by one meprin and TRAF homology domain. Multiple-sequence alignment indicated that TRAF6 was highly conserved among species, and PmTRAF6 showed 53% sequence identity to Azumapecten farreri and Mizuhopecten yessoensis. Furthermore, an amino acid sequence containing a low-complexity region was inserted in the TRAF6s from mollusk. Quantitative real-time polymerase chain reaction analysis demonstrated that PmTRAF6 was constitutively expressed in all tissues studied, with the most abundant mRNA expression in hepatopancreas and gill in P. martensii. After lipopolysaccharide stimulation, the expression of PmTRAF6 mRNA was dramatically upregulated. These results suggested that the obtained PmTRAF6 was a member of the TRAF6 family and perhaps involved in the innate immune response of pearl oyster.

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Section: Discussionmentioning

confidence: 99%

Section: Homology Analysis Of Pmtraf6mentioning

confidence: 92%