Identification and expression analysis of <i>fabp2</i> gene from common carp <i>Cyprinus carpio</i>

Chen, X. W.; Jiang, Shan

doi:10.1111/j.1095-8649.2011.03203.x

Cited by 15 publications

(6 citation statements)

References 27 publications

(28 reference statements)

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“…The same expression profile was observed in goat (Wang et al 2015). Moreover, Chen et al (2012) found that FABP2 was mainly expressed in the intestine and the brain from the common carp Cyprinus carpio but was hardly expressed in the liver, heart, pancreas and muscle. This might reflect different expression locations in different species.…”

Section: Discussionsupporting

confidence: 63%

Bioinformatics analysis and tissue-specific expression of intestinal-type fatty acid binding protein in domestic pigeons (Columba livia)

Mao

Liu

et al. 2020

Journal of Applied Animal Research

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Intestinal fatty-acid binding protein (I-FABP, also called FABP2) is involved in the uptake of dietary fatty acids in the intestine, and its binding specificity is only with long-chain fatty acid. In this study, sequences of 4 exons in pigeon FABP2 were successfully amplified. The encoded protein had a molecular weight of 15.13 kDa and the theoretical pI was 6.75. Bioinformatics analysis indicated that the protein was predicted to contain 10 potential phosphorylation sites and did not contain signal peptide structure. The evolutionary analysis suggested the amino acid sequence of pigeon FABP2 was closest to the mallard (Nippoia nippon) homologs. Meanwhile the expression of FABP2 mRNA in different ages and tissues we conducted. It showed that the expression of the FABP2 gene was the highest in the liver, followed by the small intestine and adipose tissue, while lowest in the breast muscle. Also, the expression level in the gizzard was gradually increased with aging. Our results may serve as reference to better studies of the functional characterization and structure of the FABP2 in pigeon.

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Section: Discussionsupporting

confidence: 63%

Bioinformatics analysis and tissue-specific expression of intestinal-type fatty acid binding protein in domestic pigeons (Columba livia)

Mao

Liu

et al. 2020

Journal of Applied Animal Research

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“…; Chen et al . ; Venold et al . ), and its nutritional control has also been demonstrated (Jordal et al .…”

Section: Discussionmentioning

confidence: 99%

Effects of fish oil replacement by a vegetable oil blend on digestibility, postprandial serum metabolite profile, lipid and glucose metabolism of European sea bass (Dicentrarchus labrax) juveniles

et al. 2014

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Metabolism Aquaculture, Saint-P ee-sur-Nivelle, France A digestibility trial was conducted to evaluate the effects of dietary fish oil (FO) replacement by a vegetable oil blend (VO) (rapeseed, linseed and palm oils) on nutrient digestibility, lipid absorption and transport and lipid and carbohydrate metabolic pathways of Dicentrarchus labrax juveniles. Following 36 days, the liver and intestine of fish were sampled 6 h after the last meal. Remaining fish were fed one more week and blood was collected at different postprandial time intervals. Dietary VO decreased apparent digestibility coefficients (ADC) of lipid and energy. Among the serum parameters analysed, only high-density lipoprotein was affected, being higher in VO group. Hepatic and intestinal expression of genes related to lipid transport and lipoprotein metabolism (FABP, DGAT, ApoB, ApoA4, ApoA1, MTP) showed no differences in transcript levels. No alteration in genes related to cholesterol biosynthesis (HMGCR), glucose transport (GLUT2) or metabolism (GK, PK, G6Pase and PEPCK) in the liver and intestine was found. However, hepatic transcript levels of FADS2 and elovl5 were up-regulated in VO group. In conclusion, replacement of FO by VO did not induce major alterations at molecular and biochemical levels, although a nutritional modulation of LC-polyunsaturated fatty acids biosynthesis pathway was observed.

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“…). For immunocytochemistry, larvae and tissues from adult fish were fixed in Bouin's solution and embedded in paraffin, and the 6 µm serial sections of the larvae and tissues were stained with human CYP19A antibody (Chen et al , ). Immunohistochemical analysis shows that CYP19A is distributed in the stroma cells of the connective tissue in adult fish ovaries.…”

Section: Primers Used For Carassius Auratus Gene Cloning and The Exprmentioning

confidence: 99%

Molecular characterization and expression of cyp19a gene in Carassius auratus

Chen

Jiang

2014

Journal of Fish Biology

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A cyp19a gene that contains nine exons and eight introns was identified from Carassius auratus and was mainly expressed in the ovary. The cyp19a mRNA level after hatching was initially low, but began to increase from 25 days after hatching. A number of cis-acting elements, such as the oestrogen receptor, steroidogenic factor 1 and SOX-5 recognition sites, were found in the promoter of the cyp19 gene, which possesses a promoter function confirmed by a recombination green fluorescent protein checking system in vitro.

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Identification and expression analysis of fabp2 gene from common carp Cyprinus carpio

Cited by 15 publications

References 27 publications

Bioinformatics analysis and tissue-specific expression of intestinal-type fatty acid binding protein in domestic pigeons (Columba livia)

Bioinformatics analysis and tissue-specific expression of intestinal-type fatty acid binding protein in domestic pigeons (Columba livia)

Effects of fish oil replacement by a vegetable oil blend on digestibility, postprandial serum metabolite profile, lipid and glucose metabolism of European sea bass (Dicentrarchus labrax) juveniles

Molecular characterization and expression of cyp19a gene in Carassius auratus

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