Identification and expression analysis of Sorghum bicolor gibberellin oxidase genes with varied gibberellin levels involved in regulation of stem biomass

Wang, Yongli; Sun, Jianzhong; Ali, Sameh S.; Gao, Lu; Ni, Xingnan; Li, Xia; Wu, Yanfang; Jiang, Jianxiong

doi:10.1016/j.indcrop.2019.111951

Cited by 21 publications

(12 citation statements)

References 85 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Transport of GA from the LB:LS collar to growing internodes could occur through the endodermis of LS and stem vascular bundles 87 , 89 . In addition, GA distribution in organs and tissues is shaped by enzymes such as GA 2-oxidases that mediate GA turnover and GA 20-oxidases that provide precursors for GA 3-oxidases 90 . GA 2-oxidase expression has been documented in the preligule tissue of maize 91 and at the transition zone of cell elongation in maize leaf blades 80 .…”

Section: Discussionmentioning

confidence: 99%

High planting density induces the expression of GA3-oxidase in leaves and GA mediated stem elongation in bioenergy sorghum

McKinley

Rooney

et al. 2021

Sci Rep

View full text Add to dashboard Cite

The stems of bioenergy sorghum hybrids at harvest are > 4 m long, contain > 40 internodes and account for ~ 80% of harvested biomass. In this study, bioenergy sorghum hybrids were grown at four planting densities (~ 20,000 to 132,000 plants/ha) under field conditions for 60 days to investigate the impact shading has on stem growth and biomass accumulation. Increased planting density induced a > 2-fold increase in sorghum internode length and a ~ 22% decrease in stem diameter, a typical shade avoidance response. Shade-induced internode elongation was due to an increase in cell length and number of cells spanning the length of internodes. SbGA3ox2 (Sobic.003G045900), a gene encoding the last step in GA biosynthesis, was expressed ~ 20-fold higher in leaf collar tissue of developing phytomers in plants grown at high vs. low density. Application of GA3 to bioenergy sorghum increased plant height, stem internode length, cell length and the number of cells spanning internodes. Prior research showed that sorghum plants lacking phytochrome B, a key photoreceptor involved in shade signaling, accumulated more GA1 and displayed shade avoidance phenotypes. These results are consistent with the hypothesis that increasing planting density induces expression of GA3-oxidase in leaf collar tissue, increasing synthesis of GA that stimulates internode elongation.

show abstract

Section: Discussionmentioning

confidence: 99%

High planting density induces the expression of GA3-oxidase in leaves and GA mediated stem elongation in bioenergy sorghum

McKinley

Rooney

et al. 2021

Sci Rep

View full text Add to dashboard Cite

show abstract

“…In oleaginous yeasts, lipids are synthesized via two distinct biosynthetic pathways. Lignocellulosic biomass sugars are used as a hydrophilic carbon substrate in the de novo pathway [ 200 ]. However, oils are used as a hydrophobic carbon substrate for lipid biosynthesis in the ex novo pathway.…”

Section: Biosynthetic Pathways Of Lipids By Oleaginous Yeasts For Bio...mentioning

confidence: 99%

Could termites be hiding a goldmine of obscure yet promising yeasts for energy crisis solutions based on aromatic wastes? A critical state-of-the-art review

Ali

Al-Tohamy

Mohamed

et al. 2022

Biotechnol Biofuels

Self Cite

View full text Add to dashboard Cite

Biodiesel is a renewable fuel that can be produced from a range of organic and renewable feedstock including fresh or vegetable oils, animal fats, and oilseed plants. In recent years, the lignin-based aromatic wastes, such as various aromatic waste polymers from agriculture, or organic dye wastewater from textile industry, have attracted much attention in academia, which can be uniquely selected as a potential renewable feedstock for biodiesel product converted by yeast cell factory technology. This current investigation indicated that the highest percentage of lipid accumulation can be achieved as high as 47.25% by an oleaginous yeast strain, Meyerozyma caribbica SSA1654, isolated from a wood-feeding termite gut system, where its synthetic oil conversion ability can reach up to 0.08 (g/l/h) and the fatty acid composition in yeast cells represents over 95% of total fatty acids that are similar to that of vegetable oils. Clearly, the use of oleaginous yeasts, isolated from wood-feeding termites, for synthesizing lipids from aromatics is a clean, efficient, and competitive path to achieve "a sustainable development" towards biodiesel production. However, the lacking of potent oleaginous yeasts to transform lipids from various aromatics, and an unknown metabolic regulation mechanism presented in the natural oleaginous yeast cells are the fundamental challenge we have to face for a potential cell factory development. Under this scope, this review has proposed a novel concept and approach strategy in utilization of oleaginous yeasts as the cell factory to convert aromatic wastes to lipids as the substrate for biodiesel transformation. Therefore, screening robust oleaginous yeast strain(s) from wood-feeding termite gut system with a set of the desirable specific tolerance characteristics is essential. In addition, to reconstruct a desirable metabolic pathway/network to maximize the lipid transformation and accumulation rate from the aromatic wastes with the applications of various “omics” technologies or a synthetic biology approach, where the work agenda will also include to analyze the genome characteristics, to develop a new base mutation gene editing technology, as well as to clarify the influence of the insertion position of aromatic compounds and other biosynthetic pathways in the industrial chassis genome on the expressional level and genome stability. With these unique designs running with a set of the advanced biotech approaches, a novel metabolic pathway using robust oleaginous yeast developed as a cell factory concept can be potentially constructed, integrated and optimized, suggesting that the hypothesis we proposed in utilizing aromatic wastes as a feedstock towards biodiesel product is technically promising and potentially applicable in the near future.

show abstract

“…In a potential biofuel crop, switchgrass, ectopic expression of ZmGA20ox resulted in increased growth and biomass-related traits ( Do et al, 2016 ). Recently, nine genes of GA20-ox1 were identified in sweet sorghum (bioenergy sorghum) and showed differential spatiotemporal patterns of expression while SbGA20ox1 was predominantly related to increased stem biomass and assimilates partitioning ( Wang et al, 2020 ). “Green revolution” gene GA20-oxidase is involved in the synthesis of principal biopolymer in the cell wall, i.e., cellulose in sorghum, whereas dwarf1-1 cellulose deficient and male gametophyte-dysfunctional mutant showed ablation of GA and altered expression of three CESA genes generating cellulose deficient and dwarf phenotypes ( Petti et al, 2015 ).…”

Section: Genetic Basis For Biomassmentioning

confidence: 99%

Genetic Determinants of Biomass in C4 Crops: Molecular and Agronomic Approaches to Increase Biomass for Biofuels

et al. 2022

View full text Add to dashboard Cite

Bio-based fuels have become popular being efficient, cost-effective, and eco-friendly alternatives to fossil fuels. Among plant sources exploited as feedstocks, C4 grasses, such as sugarcane, maize, sorghum, and miscanthus, are highly resourceful in converting solar energy into chemical energy. For a sustainable and reliable supply of feedstocks for biofuels, we expect dedicated bioenergy crops to produce high biomass using minimum input resources. In recent years, molecular and genetic advancements identified various factors regulating growth, biomass accumulation, and assimilate partitioning. Here, we reviewed important genes involved in cell cycle regulation, hormone dynamics, and cell wall biosynthesis. A number of important transcription factors and miRNAs aid in activation of important genes responsible for cell wall growth and re-construction. Also, environmental components interacting with genetic controls modulate plant biomass by modifying gene expression in multiple interacting pathways. Finally, we discussed recent progress using hybridization and genome editing techniques to improve biomass yield in C4 grasses. This review summarizes genes and environmental factors contributing biomass yield in C4 biofuel crops which can help to discover and design bioenergy crops adapting to changing climate conditions.

show abstract

Identification and expression analysis of Sorghum bicolor gibberellin oxidase genes with varied gibberellin levels involved in regulation of stem biomass

Cited by 21 publications

References 85 publications

High planting density induces the expression of GA3-oxidase in leaves and GA mediated stem elongation in bioenergy sorghum

High planting density induces the expression of GA3-oxidase in leaves and GA mediated stem elongation in bioenergy sorghum

Could termites be hiding a goldmine of obscure yet promising yeasts for energy crisis solutions based on aromatic wastes? A critical state-of-the-art review

Genetic Determinants of Biomass in C4 Crops: Molecular and Agronomic Approaches to Increase Biomass for Biofuels

Contact Info

Product

Resources

About