Identification and expression analysis of &amp;lt;i&amp;gt;miR-144-5p&amp;lt;/i&amp;gt; and &amp;lt;i&amp;gt;miR-130b-5p&amp;lt;/i&amp;gt; in dairy cattle

Li, Zhixiong; Wang, Hongliang; Chen, Ling; Zhai, Mengxing; Chen, Si; Li, Na; Liu, Xiaolin

doi:10.5194/aab-60-199-2017

Cited by 5 publications

(3 citation statements)

References 29 publications

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“…Interestingly, Lai et al [ 63 ] identified five significantly upregulated miRNAs (miR-21, miR-146a, miR-155, miR-222, and miR-383) with the potential to effectively differentiate between California mastitis test-positive milk and non-infected milk. In another study, miR-144-5p and miR-130b-5p were significantly downregulated and upregulated, respectively, in mammary gland tissues infected with mastitis compared to healthy tissues [ 84 ].…”

Section: Mirna Roles In Farm Animal Diseasesmentioning

confidence: 99%

miRNA Regulatory Functions in Farm Animal Diseases, and Biomarker Potentials for Effective Therapies

Ngoc

Dudemaine

Mathur

et al. 2021

IJMS

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MicroRNAs (miRNAs) are small endogenous RNAs that regulate gene expression post-transcriptionally by targeting either the 3′ untranslated or coding regions of genes. They have been reported to play key roles in a wide range of biological processes. The recent remarkable developments of transcriptomics technologies, especially next-generation sequencing technologies and advanced bioinformatics tools, allow more in-depth exploration of messenger RNAs (mRNAs) and non-coding RNAs (ncRNAs), including miRNAs. These technologies have offered great opportunities for a deeper exploration of miRNA involvement in farm animal diseases, as well as livestock productivity and welfare. In this review, we provide an overview of the current knowledge of miRNA roles in major farm animal diseases with a particular focus on diseases of economic importance. In addition, we discuss the steps and future perspectives of using miRNAs as biomarkers and molecular therapy for livestock disease management as well as the challenges and opportunities for understanding the regulatory mechanisms of miRNAs related to disease pathogenesis.

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Section: Mirna Roles In Farm Animal Diseasesmentioning

confidence: 99%

miRNA Regulatory Functions in Farm Animal Diseases, and Biomarker Potentials for Effective Therapies

Ngoc

Dudemaine

Mathur

et al. 2021

IJMS

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“…[31] miR-320a and miR-320b identi ed during S. uberis mastitis due to their roles in enhance immune system. In another investigation, miR-144-5p and miR-130b-5p were signi cantly down-regulated and up-regulated respectively, in mastitis animals [32]. Network analysis of differential expression genes indicated that TNF pathway had positive relationships with genes involved with immune system function [33].…”

mentioning

confidence: 94%

A meta-analysis of differentially expressed microRNA during mastitis disease in dairy cattle

Panahi,

hasanpour,

ghahramani

et al. 2023

Preprint

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Background: Bovine mastitis is an important inflammation disease that affects the mammary gland and causing adverse effects on the quality and quantity of the produced milk, leads to a major economic lost in dairy industry. Streptococcus uberisis one of the bacteria commonly responsible for inducing mastitis in dairy cattle. Susceptibility to develop mastitis is a complex multifactorial phenotype and the improvement of the miRNAs and their target genes has not been comprehensively illustrated. Methods and Results:The purpose of this investigation was to perform a meta-analysis of the miRNAs expression profiling datasets to detect the key miRNAs, targets, and regulatory networks associated with mastitis. To this, publicly available miRNA datasets belong to three experiments on dairy cattle which challenged with S. uberiswere included in our meta-analyzed. The identified differentially expressed miRNAs were used in TargetScan to identify their target genes. The functional impacts of the meta-miRNAs were further analyzed using Gene ontology and Protein-Protein Interaction network analysis. Three meta-miRNAs, namely bta-miR-98, bta-miR-138 and bta-miR-193a-3p, were obtained to be associated with the progress of the immune system and cell differentiation of the mammary gland during the mastitis. A total of 2061 target genes were identified that which bta-miR-98, bta-miR-138 and bta-miR-193a-3p were regulated 1121, 268 and 672 target genes respectively. Gene ontology analysis results were represented 237 biological process, 41 molecular function, 54 cellular component roles and nine KEGG pathways in mastitis disease. A total of 319, 113 and 124 target genes for bta-miR-98, bta-miR-193a-3p and bta-miR-138, respectively were inputted to cytoscape. The resulted network analysis showed that bta-miR-98 and bta-miR-138 have nine, bta-miR-138 and bta-miR-193a-3p have six, and bta-miR-193a-3p and bta-miR-98 have four common target genes. Twenty-one common genes were revealed by combing 360 common meta-genes in our previous research and 2061 meta-miRNA target genes. The procedure reported in this research offers a comprehensive scheme for the identification of the key miRNAs and target genes in mastitis disease by using global transcriptome data, meta-analysis, gene ontology, enrichment analysis and protein protein interaction. Conclusion: The findings of the current work suggest miRNAs are crucial amplifiers of inflammatory response by controlling metabolic pathway and inhibitors of several biological processes during S. uberis infection.

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“…MicroRNA dysregulation has been reported to be associated with several traits in cattle, for example resistance to mastitis in dairy cattle (Li et al, 2017). Additionally, various miRNA regulome polymorphisms -within miRNA genes (miR-SNPs; single nucleotide polymorphisms), regulatory regions (miR-rSNPs), target sites (miR-TS-SNPs), and silencing machinery (miR-SM-SNPs) have also been associated with phenotypic traits and diseases Georges et al, 2007;Hrovatin and Kunej, 2018).…”

mentioning

confidence: 99%

The microRNA gene <i>bta-mir-2313</i> in cattle: an atlas of regulatory elements and an association analysis with growth and carcass traits in the Slovenian Simental cattle breed

Malovrh¹,

Kunej²,

Kovač³

et al. 2018

Arch. Anim. Breed.

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Abstract. MicroRNAs (miRNA) are a class of non-coding RNAs important in posttranscriptional regulation of target genes. Regulation requires complementarity between the target mRNA and the miRNA region responsible for their recognition and binding, also called the seed region. Previous studies have proven that expression profiles and genetic variations of miRNA genes (miR-SNP; SNP -single nucleotide polymorphism) and their target sites (miR-TS-SNPs) have an impact on phenotypic variation and disease susceptibility in human, animal models, and livestock. MicroRNA-associated polymorphisms therefore represent biomarker potential for phenotypic traits in livestock. Effects of miRNA gene polymorphisms on phenotypic traits have been studied in several animal species but much less in cattle. The aim of the present study was therefore to analyze the genetic variability in the bta-mir-2313 gene and test associations with growth and carcass traits of the Slovenian Simmental cattle breed. Additionally, validated and predicted genomic information related to the miRNA gene bta-mir-2313 has been obtained and presented as an atlas of miRNA regulatory elements. Sanger sequencing has been used for biomarker development and genotyping of 145 animals of Slovenian dual-purpose Simmental cattle. Out of nine known polymorphisms located within pre-miRNA regions, one mature miRNA seed SNP was polymorphic in the Slovenian Simmental cattle breed. An additional three polymorphisms were identified within the flanking pri-miRNA regions. There was no significant effect of polymorphisms on 18 tested fattening and carcass traits; however, validated polymorphisms could now be tested in association with other traits in other cattle populations. The microRNA gene bta-mir-2313 warrants further genetic and functional analyses since it overlaps with a large number of quantitative trait loci (QTL), has over 3100 predicted targets and highly polymorphic mature seed regions, and is located within protein-coding gene GRAMD1B, previously associated with production traits in cattle. Mature miRNA seed SNPs present important genomic loci for functional studies because they could affect the gain/loss of downstream targets and should be systematically studied in cattle.

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Identification and expression analysis of <i>miR-144-5p</i> and <i>miR-130b-5p</i> in dairy cattle

Cited by 5 publications

References 29 publications

miRNA Regulatory Functions in Farm Animal Diseases, and Biomarker Potentials for Effective Therapies

miRNA Regulatory Functions in Farm Animal Diseases, and Biomarker Potentials for Effective Therapies

A meta-analysis of differentially expressed microRNA during mastitis disease in dairy cattle

The microRNA gene <i>bta-mir-2313</i> in cattle: an atlas of regulatory elements and an association analysis with growth and carcass traits in the Slovenian Simental cattle breed

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Identification and expression analysis of &lt;i&gt;miR-144-5p&lt;/i&gt; and &lt;i&gt;miR-130b-5p&lt;/i&gt; in dairy cattle

Cited by 5 publications

References 29 publications

miRNA Regulatory Functions in Farm Animal Diseases, and Biomarker Potentials for Effective Therapies

miRNA Regulatory Functions in Farm Animal Diseases, and Biomarker Potentials for Effective Therapies

A meta-analysis of differentially expressed microRNA during mastitis disease in dairy cattle

The microRNA gene &lt;i&gt;bta-mir-2313&lt;/i&gt; in cattle: an atlas of regulatory elements and an association analysis with growth and carcass traits in the Slovenian Simental cattle breed

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Product

Resources

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Identification and expression analysis of <i>miR-144-5p</i> and <i>miR-130b-5p</i> in dairy cattle

The microRNA gene <i>bta-mir-2313</i> in cattle: an atlas of regulatory elements and an association analysis with growth and carcass traits in the Slovenian Simental cattle breed