Identification and Expression Analysis of DUF4228 Domain Containing (DDP) Genes in Potato Under Abiotic and Phytohormone Stress

Abstract

DUF4228 (Domain of unknown function 4228) proteins are widely distributed in plants and performed a vital role in abiotic stress response. In potato (Solanum tuberosum), the study of the DUF4228 gene family is sparse. To understant the role of DUF4228 in potato a comprehensive genome-wide analysis was carried out in potato genome. Further, the StDUAF4228 genes relative expression was also evaluated in various plant tissues. In the present study identified 31 StDUF4228 genes were clustered into six groups. The promoter analysis of StDUF4228 revealed various cis-acting regulatory components response to abiotic and phytohormones stresses. Under selection pressure, gene duplication also showed various positive and purifying selections in StDUF4228 genes. Expression profiling indicated that StDUF4228 genes were broadly expressed in various tissues and organs in potato. Moreover, the StDUF4228 genes profiles expression was characterized through RNA-sequence analysis data under salt and heat treatments. In potato, StDUF4228-4, StDUF4228-14, StDUF4228-21, and StDUF4228-30 represented a high expression under salt and heat stress.Furthermore, 23 genes under IAA treatment and 18 under ABA treatment, showed that IAA and ABA played a vital role in stresses. The RT-qPCR and expression profilling exhibited high expression in root tissues. Moreover multiple miRNA target StDUF4228 genes in potato.These expression results provide primary reference data and functional analysis data for other commercial crops.

Genome-Wide Identification, and In-Silico Expression Analysis of YABBY Gene Family in Response to Biotic and Abiotic Stresses in Potato (Solanum tuberosum)

“…Gene duplication is the main driver of genome expansion in species. Variations in the gene family members across different eukaryotic species might be because of the process of evolution [ 53 ]. Moreover, the dual synteny blocks of potato were constructed with three crops, i.e., Arabidopsis, tomato, and chili, separately to determine the connection between them.…”

Identification and analysis of genomic regions influencing leaf morpho-physiological traits related to stress responses inDioscorea alata

“…A previous report suggests that disruption of ATPbinding cassette transporters causes deregulation of stomatal opening and increases drought susceptibility [86]; therefore, variation in Dioal.12G033500 function or expression could explain the photosynthesis variation in the GWAS panel and could be an excellent candidate for further functional verification in D. alata. Retrotransposon protein [87], Domain of unknown function (DUF4228) [88,89], Non-specific serine/threonine protein kinase [90,91], DNA/RNA helicase protein [92,93] have also been characterized for their potential role in coping with stress conditions and maintaining the plant growth. Gene expression data facilitate the identification of candidate genes from GWAS projects since genetic variants falling in genic or intergenic regions often lead to non-functional genes or altered gene expression.…”