Identification and Expression Analysis of DFR Gene Family in Brassica napus L.

Qian, Xingzhi; Wenyin, Zheng; Hu, Jian; Ma, Jie; Sun, Mei; Li, Yong; Liu, Nian; Chen, Tianhua; Wang, Meiqi; Hou, Xiaolu; Cai, Qingao; Ye, Zhaoshun; Zhang, Fugui; Zhu, Zhiliang

doi:10.3390/plants12132583

Cited by 3 publications

(1 citation statement)

References 54 publications

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“…This indicates that the localization of the DFR gene is species-specific, and its localization differed from species to species; however, its role in scavenging ROS could remain same across species. In Brassica napus, a total of 26 BnDFR genes were localized in chloroplasts, cytoplasm, nuclei, and mitochondria [51], which is in accordance with our study. Interestingly, in our transcriptome analysis, genes (such as DFR, LAR, AT1, MAT, CHS2, CYP73A12, CHS3, BAHD1, and FLS) in the biosynthesis of secondary metabolite pathways were identified to be upregulated after 20 d exposure to different Mg levels (Figure 13), suggesting spontaneous activation of precursors involved in the biosynthesis of various antioxidants and flavonoids, which assist in counteracting the detrimental effects of Mg stresses.…”

Section: Mulberry Response To Mg Imbalances Trigger Antioxidants and ...supporting

confidence: 92%

Comparative Physio-Biochemical and Transcriptome Analyses Reveal Contrasting Responses to Magnesium Imbalances in Leaves of Mulberry (Morus alba L.) Plants

Shi,

Jin,

Ackah

et al. 2024

Antioxidants

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Magnesium (Mg) deficiency is a major factor limiting the growth and development of plants. Mulberry (Morus alba L.) is an important fruit tree crop that requires Mg for optimal growth and yield, especially in acid soils. However, the molecular mechanism of Mg stress tolerance in mulberry plants remains unknown. In this study, we used next-generation sequencing technology and biochemical analysis to profile the transcriptome and physiological changes of mulberry leaves under different Mg treatments (deficiency: 0 mM, low: 1 mM, moderate low: 2 mM, sufficiency: 3 mM, toxicity: 6 mM, higher toxicity: 9 mM) as T1, T2, T3, CK, T4, T5 treatments, respectively, for 20 days. The results showed that Mg imbalance altered the antioxidant enzymatic activities, such as catalase (CAT), peroxidase (POD), and superoxide dismutase (SOD), and non-enzymatic, including soluble protein, soluble sugar, malondialdehyde (MDA), and proline (PRO), contents of the plant. The Mg imbalances disrupted the ultrastructures of the vital components of chloroplast and mitochondria relative to the control. The transcriptome data reveal that 11,030 genes were differentially expressed (DEGs). Genes related to the photosynthetic processes (CAB40, CAB7, CAB6A, CAB-151, CAP10A) and chlorophyll degradation (PAO, CHLASE1, SGR) were altered. Antioxidant genes such as PER42, PER21, and PER47 were downregulated, but DFR was upregulated. The carbohydrate metabolism pathway was significantly altered, while those involved in energy metabolism processes were perturbed under high Mg treatment compared with control. We also identified several candidate genes associated with magnesium homeostasis via RT-qPCR validation analysis, which provided valuable information for further functional characterization studies such as promoter activity assay or gene overexpression experiments using transient expression systems.

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Section: Mulberry Response To Mg Imbalances Trigger Antioxidants and ...supporting

confidence: 92%

Comparative Physio-Biochemical and Transcriptome Analyses Reveal Contrasting Responses to Magnesium Imbalances in Leaves of Mulberry (Morus alba L.) Plants

Shi,

Jin,

Ackah

et al. 2024

Antioxidants

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Genome-wide identification and characterization of flavonol synthase (FLS) gene family in Brassica vegetables and their roles in response to biotic and abiotic stresses

Yue,

Zhong,

Kang

et al. 2024

Scientia Horticulturae

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Genome-wide identification of putative dihydroflavonol 4-reductase (DFR) gene family in eight Solanaceae species and expression analysis in Solanum lycopersicum

Li,

Zhang,

Liu

et al. 2023

PeerJ

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Dihydroflavonol 4-reductase (DFR; EC1.1.1.219) is an important rate-limiting enzyme in the plant flavonoid pathway toward both anthocyanins and proanthocyanidins. Although DFR genes have been isolated from multiple plants and their functions have been well characterized in some plants, little is known about DFRs in Solanaceae species. Therefore, in this study, we performed genome-wide analysis and identified 6, 5, 4, 5, 5, 6, 6 and 5 DFR gene family members in eight Solanaceae species (S. lycopersicum, S. pennellii, S. tuberosum, S. melongena, C. annuum, N. tabacum, P. inflata, and P. axillaris) respectively. The putative DFR genes were systematically identified using bioinformatics to predict their protein properties, cellular location, phylogenetic relationships, gene structure, conserved motifs, and cis-acting elements in the promoters. Furthermore, quantitative real-time PCR (qRT-PCR) was used to identify the expression pattern of DFRs in tomato. We classified all DFRs into five groups based on their phylogenetic features. Sequence analysis showed that all encoded DFR protein sequences possess a highly conserved NAD-dependent epimerase/dehydratase. In addition, almost all the members of each group displayed similar gene structures and motif distributions, which might be related to their identical executive functions. All 42 DFRs possess a series of light-responsive, phytohormone-responsive, MYB-responsive, stress-responsive, and tissue-specific expression-related cis-elements in the promoter sequences. qRT-PCR analysis showed that tomato DFRs were expressed in many different organs. This study will provide a theoretical basis for further investigation of the function of DFRs in Solanaceae.

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Identification and Expression Analysis of DFR Gene Family in Brassica napus L.

Cited by 3 publications

References 54 publications

Comparative Physio-Biochemical and Transcriptome Analyses Reveal Contrasting Responses to Magnesium Imbalances in Leaves of Mulberry (Morus alba L.) Plants

Comparative Physio-Biochemical and Transcriptome Analyses Reveal Contrasting Responses to Magnesium Imbalances in Leaves of Mulberry (Morus alba L.) Plants

Genome-wide identification and characterization of flavonol synthase (FLS) gene family in Brassica vegetables and their roles in response to biotic and abiotic stresses

Genome-wide identification of putative dihydroflavonol 4-reductase (DFR) gene family in eight Solanaceae species and expression analysis in Solanum lycopersicum

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