Identification and expression analysis of Pofst3 suggests a role during Pleurotus ostreatus primordia formation

Qi, Yuancheng; Chen, Huijuan; Zhang, Mengke; Wen, Qing; Qiu, Liyou; Shen, Jinwen

doi:10.1016/j.funbio.2018.12.008

Cited by 20 publications

(12 citation statements)

References 38 publications

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“…fst3 is also reported to be able to control the number of fruit body by inhibiting mushroom clustering formation, which is extremely important for fungal survival as it will ensure that at least one fruit‐body fully develops under unfavourable conditions (Sakamoto, 2018) such as low oxygen. Deletion of fst3 will lead to a small size of fruit bodies compared to the wild type (Ohm et al ., 2010; Qi et al ., 2019). Similarly, the expression of gat 1 is also significantly downregulated under lack of oxygen compared to aerobic conditions, especially in the fruit body.…”

Section: Discussionmentioning

confidence: 99%

Effect of oxygen concentrations and branched‐chain amino acids on the growth and development of sub‐seafloor fungus, Schizophyllum commune 20R‐7‐F01

Arifeen

et al. 2021

Environmental Microbiology

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Summary Fungi have been reported to be the dominant eukaryotic group in anoxic sub‐seafloor sediments, but how fungi subsist in the anoxic sub‐marine sedimental environment is rarely understood. Our previous study demonstrated that the fungus, Schizophyllum commune 20R‐7‐F01 isolated from a ~2 km sediment below the seafloor, can grow and produce primordia in the complete absence of oxygen with enhanced production of branched‐chain amino acids (BCAAs), but the primordia cannot be developed into fruit bodies without oxygen. Here, we present the individual and synergistic effects of oxygen and BCAAs on the fruit‐body development of this strain. It was found that the fungus required a minimum oxygen concentration of 0.5% pO2 to generate primordia and 1% pO2 to convert primordia into mature fruit body. However, if BCAAs (20 mM) were added to the medium, the primordium could be developed into fruit body at a lower oxygen concentration up to 0.5% pO2 where genes fst4 and c2h2 playing an important role in compensating oxygen deficiency. Moreover, under hypoxic conditions, the fungus showed an increase in mitochondrial number and initiation of auto‐phagocytosis. These findings suggest that the fruit‐body formation of S. commune may have multiple mechanisms, including energy and amino acid metabolism in response to oxygen concentrations.

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Section: Discussionmentioning

confidence: 99%

Effect of oxygen concentrations and branched‐chain amino acids on the growth and development of sub‐seafloor fungus, Schizophyllum commune 20R‐7‐F01

Arifeen

et al. 2021

Environmental Microbiology

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“…Fst3 and fst4 were the transcription factor genes that expressed in the primordium or mature fruiting stage of Schizophyllum commune . Fst3 can inhibit the formation of redundant primordia clusters, and fst4 plays a role in the transformation of Schizophyllum commune from vegetative growth stage to reproductive growth stage [3] and the Pofst3 in P. ostreatus also plays the same role as fst3 in Schizophyllum commune [16]. After ASA was added, the expression of PoWC‐1 , PoWC‐2 , Pofst3, and Pofst4 genes changed, and the expression of PoWC‐2 and Pofst4 was significantly upregulated in the primordium stage (Figure 5).…”

Section: Discussionmentioning

confidence: 99%

“…Transcription factor fst4 can promote the formation of primordia of Schizophyllum commune , but fst3 does the opposite [3]. We found that the Pofst3 in P. ostreatus also plays the same role as fst3 in Schizophyllum commune [16].…”

Section: Introductionmentioning

confidence: 99%

Effects of exogenous ascorbic acid on the mycelia growth and primordia formation of Pleurotus ostreatus

Zhang

et al. 2021

J Basic Microbiol

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Primordia formation is the first and most critical step in the development of fruiting bodies of edible fungi. In this study, the effects of exogenous ascorbic acid (ASA) on the Pleurotus ostreatus mycelia growth and primordia formation were researched and the results showed that the growth rate of P. ostreatus mycelia was accelerated and the time of primordia formation was advanced. The protein content and ascorbate oxidase (AAO) activity analysis showed that with the increase of ASA concentration, the protein content of mycelia first decreased and then increased, and in a certain concentration range, exogenous ASA could significantly promote the activity of AAO. Further expression analysis of the development regulating genes (Pofst3 and Pofst4) as well as blue light receptor coding genes (PoWC‐1 and PoWC‐2) showed the expression levels of those four genes all changed after the exogenous ASA addition, which indicated that the expression changes of PoWC‐1 and PoWC‐2, two key genes in the light morphogenesis, might affect the expression levels of development regulating genes Pofst3 and Pofst4, so as to lead to the formation of primordia in advance.

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“…The cDNA was synthesized from the total RNA using a PrimeScript RT Reagent Kit with gDNA Eraser (TaKaRa, Dalian, China). Subsequently, the RT-qPCR assay was performed according to a previous report [ 21 ]. Actin was used as an internal control [ 21 ].…”

Section: Methodsmentioning

confidence: 99%

Effects of Heat Stress and Exogenous Salicylic Acid on Secondary Metabolites Biosynthesis in Pleurotus ostreatus (Jacq.) P. Kumm

Chai

Chen

et al. 2022

Life

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Pleurotus ostreatus (Jacq.) P. Kumm has high medicinal value, but few studies exist on regulating secondary metabolite biosynthesis. Environmental factors play a substantial role in the accumulation of microbial secondary metabolites. In this study, the effects of heat stress (24 h) and salicylic acid (0.05 mmol/L) treatment on the secondary metabolism of P. ostreatus were analyzed by metabolome, transcriptome, and gene differential expression analysis. Metabolome and transcriptome analyses showed that salicylic acid significantly increased the accumulation of antibiotics and polyketones, while heat stress increased the accumulation of flavonoids, polyketones, terpenoids, and polysaccharides. The content and the biosynthetic genes expression of heparin were markedly increased by heat stress, and the former was increased by 4565.54-fold. This study provides a reference for future studies on secondary metabolite accumulation in edible fungi.

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Identification and expression analysis of Pofst3 suggests a role during Pleurotus ostreatus primordia formation

Cited by 20 publications

References 38 publications

Effect of oxygen concentrations and branched‐chain amino acids on the growth and development of sub‐seafloor fungus, Schizophyllum commune 20R‐7‐F01

Effect of oxygen concentrations and branched‐chain amino acids on the growth and development of sub‐seafloor fungus, Schizophyllum commune 20R‐7‐F01

Effects of exogenous ascorbic acid on the mycelia growth and primordia formation of Pleurotus ostreatus

Effects of Heat Stress and Exogenous Salicylic Acid on Secondary Metabolites Biosynthesis in Pleurotus ostreatus (Jacq.) P. Kumm

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