Identification and expression analysis of cDNA encoding chitinase-like protein (CLP) gene in Japanese scallop Mizuhopecten yessoensis

Abstract

ABSTRACT. Chitinase-like proteins (CLP) are important members of the glycoside hydrolase family 18 (GH18) and are involved in growth control and remodeling processes. In this study, a CLP transcript was isolated and sequenced from the Japanese scallop (Mizuhopecten yessoensis) after screening expressed sequence tags. The full-length complementary DNA of M. yessoensis CLP (My-Clp1) was 1555 bp in length, consisting of a 75-bp 5'-untranslated region (UTR), a 160-bp 3'-UTR, and a 1320-bp open reading frame bearing characteristics of the GH18 family. The My-Clp1 protein was well conserved, with similar domain structures and architecture across species (e.g., from mollusks to mammals). Expression analysis in healthy tissues and across developmental stages revealed a strong preference for expression; My-Clp1 was abundantly expressed in the mantle and throughout metamorphosis, which suggests the involvement of My-Clp1 in the synthesis of extracellular components, and tissue degeneration and remodeling. My-Clp1 expression was induced after infection with a bacterial pathogen, Vibrio anguillarum, suggesting its involvement in immunity against this intracellular pathogen.

The Inhibition of Ocean Acidification on the Formation of Oyster Calcified Shell by Regulating the Expression of Cgchs1 and Cgchit4

“…Phylogenic analysis also showed that Cg chs1 was close to chitin synthase from invertebrates such as B. glabrata, M. yessoensis (Bulawa, 1992; Luo et al, 2015). Cg chit4 exhibited high sequence similarity (41–52%) with chitinase from other vertebrates and invertebrates, and it was clustered with chitinases from invertebrates such as P. fucata and H. cumingii (Gao et al, 2014; Li et al, 2017). These results indicated that Cg chs1 and Cg chit4 might belong to the family of chitin synthase and chitinase in molluscs, respectively.…”

Chitotriosidase: a marker and modulator of lung disease

“…In Drosophila, IDGF is essential for larval and adult moulting as it organises extracellular matrix formation at barrier tissues [54]. In Japanese scallops, the expression of My-Clp1 was observed in various developmental stages of metamorphosis, which suggest its roles in tissue degeneration and remodelling [55]. In oysters, Cg-Clp1 and Cg-Clp2 were reported to be involved in immune responses to bacterial lipopolysaccharide challenge [53].…”

Chitinase-like proteins as regulators of innate immunity and tissue repair: helpful lessons for asthma?

“…For example, Drosophila express imaginal disc growth factor (IDGF) proteins, which are ~25% homologous to mammalian CLPs (Figure 1) and recent studies have characterised functional readouts for IDGFs during nematode infection, wound injury and healing [17,18]. Similarly mollusk CLPs including My-Clp1 from Japanese scallops and Cg-Clp1 and -Clp2 from oysters appear equally involved in developmental tissue remodelling and immune defence [19,20]. CLPs across species are also broadly expressed in similar cell types ( Table 1).…”

“…Mutation of IDGF3 leaves its host more susceptible to nematode or bacterial infection [17] and microarray analysis demonstrated that IDGF2 expression resulted in activation of innate immune genes associated with phagocytosis [18]. Similarly, following bacterial infection, mollusks rapidly upregulate My-Clp1, a CLP gene with implicated roles in innate defence and pathogen recognition [19]. The complexities of specific CLP functions still need to be untangled but overall data suggests prominent roles in regulating innate immune responses and/or pathogen recognition.…”