Identification and Expression Analysis of Candidate Genes Associated with Defense Responses to Phytophthora capsici in Pepper Line “PI 201234”

Wang, Ping-Yong; Liu, Xiaodan; Guo, Junhui; Liu, Chen; Fu, Nan; Shen, Huolin

doi:10.3390/ijms160511417

Cited by 26 publications

(18 citation statements)

References 70 publications

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“…8). However, the magnitude of the fold changes varied between RNA-seq and qRT-PCR experiments, and this phenomenon also appeared in several previous studies (Cao et al, 2018;Dong et al, 2017b;Wang et al, 2015;Zhang et al, 2018b). This may be due to sample collection for previous RNAseq and qPCR experiments at different times, or differences in RNA extraction or cDNA synthesis.…”

Section: Discussionsupporting

confidence: 52%

Peer Review #1 of "MYB transcription factors in alfalfa (Medicago sativa): genome-wide identification and expression analysis under abiotic stresses (v0.1)"

2019

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Background. Alfalfa is the most widely cultivated forage legume and one of the most economically valuable crops in the world. Its survival and production are often hampered by environmental changes. However, there are few studies on stress-resistance genes in alfalfa because of its incomplete genomic information and rare expression profile data. The MYB proteins are characterized by a highly conserved DNA-binding domain, which is large, functionally diverse, and represented in all eukaryotes. The role of MYB proteins in plant development is essential; they function in diverse biological processes, including stress and defense responses, and seed and floral development. Studies on the MYB gene family have been reported in several species, but they have not been comprehensively analyzed in alfalfa. Methods. To identify more comprehensive MYB transcription factor family genes, the sequences of 168 Arabidopsis thaliana, 430 Glycine max, 185 Medicago truncatula, and 130 Oryza sativa MYB proteins were downloaded from the Plant Transcription Factor Database. These sequences were used as queries in a BLAST search against the Medicago sativa proteome sequences provided by the Noble Research Institute. Results. In the present study, a total of 265 MsMYB proteins were obtained, including 50 R1-MYB, 186 R2R3-MYB, 26 R1R2R3-MYB, and three atypical-MYB proteins. These predicted MsMYB proteins were divided into 12 subgroups by phylogenetic analysis, and gene ontology (GO) analysis indicated that most of the MsMYB genes are involved in various biological processes. The expression profiles and quantitative real-time PCR analysis indicated that some MsMYB genes might play a crucial role in the response to abiotic stresses. Additionally, a total of 170 and 914 predicted protein-protein and protein-DNA interactions were obtained, respectively. The interactions between MsMYB043 and MSAD320162, MsMYB253 and MSAD320162, and MsMYB253 and MSAD308489 were confirmed by a yeast two-hybrid system. This work provides information on the MYB family in alfalfa that was previously lacking and might promotes the cultivation of stress-resistant alfalfa.

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Section: Discussionsupporting

confidence: 52%

Peer Review #1 of "MYB transcription factors in alfalfa (Medicago sativa): genome-wide identification and expression analysis under abiotic stresses (v0.1)"

2019

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“…Recent studies have indicated that a novel peroxidase ( CanPOD ) and oxysterol-binding protein ( CanOBP ) genes, which are involved in the defense response to P. capsici infection, exhibit expression patterns similar to these CaSBP s ( Liu, 2009 ; Wang et al, 2013b ). Moreover, similar expression patterns are also found in some defense-related genes – such as the disease-associated protein gene ( CABPR1 ), β-1,3-glucanase gene ( CABGLU ), and peroxidase gene (CAPO1 ) – in pepper roots after inoculation with compatible and incompatible P. capsici ( Wang, 2013 ). However, according to Kim and Hwang (2000) , the expression of CABPR1 is higher in the compatible interaction than in the incompatible interaction.…”

Section: Discussionmentioning

confidence: 72%

Genome-Wide Identification and Analysis of the SBP-Box Family Genes under Phytophthora capsici Stress in Pepper (Capsicum annuum L.)

Zhang

Jin

et al. 2016

Front. Plant Sci.

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SQUAMOSA promoter binding protein (SBP)-box genes encode plant-specific transcription factors that are extensively involved in many physiological and biochemical processes, including growth, development, and signal transduction. However, pepper (Capsicum annuum L.) SBP-box family genes have not been well characterized. We investigated SBP-box family genes in the pepper genome and characterized these genes across both compatible and incompatible strain of Phytophthora capsici, and also under different hormone treatments. The results indicated that total 15 members were identified and distributed on seven chromosomes of pepper. Phylogenetic analysis showed that SBP-box genes of pepper can be classified into six groups. In addition, duplication analysis within pepper genome, as well as between pepper and Arabidopsis genomes demonstrated that there are four pairs of homology of SBP-box genes in the pepper genome and 10 pairs between pepper and Arabidopsis genomes. Tissue-specific expression analysis of the CaSBP genes demonstrated their diverse spatiotemporal expression patterns. The expression profiles were similarly analyzed following exposure to P. capsici inoculation and hormone treatments. It was shown that nine of the CaSBP genes (CaSBP01, 02, 03, 04, 05, 06, 11, 12, and 13) exhibited a dramatic up-regulation after compatible HX-9 strain (P. capsici) inoculation, while CaSBP09 and CaSBP15 were down-regulated. In case of PC strain (P. capsici) infection six of the CaSBP genes (CaSBP02, 05, 06, 11, 12, and 13) were arose while CaSBP14 was down regulated. Furthermore, Salicylic acid, Methyl jasmonate and their biosynthesis inhibitors treatment indicated that some of the CaSBP genes are potentially involved in these hormone regulation pathways. This genome-wide identification, as well as characterization of evolutionary relationships and expression profiles of the pepper CaSBP genes, will help to improve pepper stress tolerance in the future.

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“…As Figure 3, Figure 6, and Figure 7 showed, CaSBP12 plays a negative regulatory role in the process of P. capsici infection. Therefore, the disease index percent was also calculated after inoculation with the HX-9 strain using the root-drench method as described by Wang (2013) [20]. After inoculation with the HX-9 strain, the infection was monitored at day 16.…”

Section: Resultsmentioning

confidence: 99%

“…Zoospore concentration was counted and adjusted to 1 × 10 5 cfu/mL using the method described by Jin et al (2016) [1]. Then, 5 mL of this zoospore was used to inoculate the CaSBP12 -silenced and control pepper plants using the root-drench method as described by Wang (2013) [20].…”

Section: Methodsmentioning

confidence: 99%

“…Pepper seedlings at the two true leaves (40 days after sowing) stage were used for the silencing of CaSBP12 as described by Zhang et al (2013) [16]. All the injected plants were grown in a growth chamber with the having same growing conditions as described by Wang (2013) [20]. Forty-five days post-infiltration, leaf and root samples from the control and silenced plants were collected to measure the silencing efficiency.…”

Section: Methodsmentioning

confidence: 99%

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A Novel Transcription Factor CaSBP12 Gene Negatively Regulates the Defense Response against Phytophthora capsici in Pepper (Capsicum annuum L.)

Zhang

Ali

Feng

et al. 2018

IJMS

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SBP-box (Squamosa-promoter binding protein) genes are a type of plant-specific transcription factor and play important roles in plant growth, signal transduction and stress response. However, little is known about the SBP-box genes in pepper (CaSBP), especially in the process of Phytophthora capsici infection. In this study, a novel gene (CaSBP12) was selected from the CaSBP gene family, which was isolated from the pepper genome database in our previous study. The CaSBP12 gene was located in the nucleus of the cell and its silencing in the pepper plant enhanced the defense response against Phytophthora capsici infection. After inoculation with Phytophthora capsici, the root activity of the CaSBP12-silenced plants is compared to control plants, while malondialdehyde (MDA) content is compared viceversa. Additionally, the expression of defense related genes (CaPO1, CaSAR8.2, CaBPR1, and CaDEF1) in the silenced plants were induced to different degrees and the peak of CaSAR8.2 and CaBPR1 were higher than that of CaDEF1. The CaSBP12 over-expressed Nicotiana benthamiana plants were more susceptible to Phytophthora capsici infection with higher EC (electrical conductivity) and MDA contents as compared to the wild-type. The relative expression of defense related genes (NbDEF, NbNPR1, NbPR1a, and NbPR1b) in transgenic and wild-type Nicotiana benthamiana plants were induced, especially the NbPR1a and NbPR1b. In conclusion, these results indicate that CaSBP12 gene negative regulates the defense response against Phytophthora capsici infection which suggests their potentially significant role in plant defense. To our knowledge, this is the first report on CaSBP gene which negative regulate defense response.

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Identification and Expression Analysis of Candidate Genes Associated with Defense Responses to Phytophthora capsici in Pepper Line “PI 201234”

Cited by 26 publications

References 70 publications

Peer Review #1 of "MYB transcription factors in alfalfa (Medicago sativa): genome-wide identification and expression analysis under abiotic stresses (v0.1)"

Peer Review #1 of "MYB transcription factors in alfalfa (Medicago sativa): genome-wide identification and expression analysis under abiotic stresses (v0.1)"

Genome-Wide Identification and Analysis of the SBP-Box Family Genes under Phytophthora capsici Stress in Pepper (Capsicum annuum L.)

A Novel Transcription Factor CaSBP12 Gene Negatively Regulates the Defense Response against Phytophthora capsici in Pepper (Capsicum annuum L.)

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