Identification and expression analysis of APETALA1 homologues in poplar

Chen, Zhong; Yang, Xiong; Su, Xiaoxing; Rao, Pian; Gao, Kai; Lei, Bingqi; An, Xinmin

doi:10.1007/s11738-015-1805-z

Cited by 8 publications

(4 citation statements)

References 56 publications

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“…Raf.) ( Sun et al, 2014 ), Birch ( Betula platyphylla ) ( Huang et al, 2014 ) and poplar ( Populus tomentosa ) ( Chen et al, 2015 ). Through mainly examining their expression patterns and their effects on heterologous systems, most of these genes have been suggested to affect either the flowering process or floral organ formation.…”

Section: Introductionmentioning

confidence: 99%

DOAP1 Promotes Flowering in the Orchid Dendrobium Chao Praya Smile

et al. 2017

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APETALA1 (AP1) encodes a key MADS-box transcription factor that specifies the floral meristem identity on the flank of the inflorescence meristem, and determines the identity of perianth floral organs in the model plant Arabidopsis thaliana. Orchids are members of the Orchidaceae, one of the largest families of angiosperms. Although the expression patterns of a few AP1-like genes in orchids have been reported, their actual functions in orchid reproductive development are so far largely unknown. In this study, we isolated and characterized an AP1 ortholog, DOAP1, from Dendrobium Chao Praya Smile. DOAP1 was highly expressed in reproductive tissues, including inflorescence apices and flowers at various developmental stages. Overexpression of DOAP1 resulted in early flowering in Arabidopsis, and was able to rescue the floral organ defects of Arabidopsis ap1 mutants. Moreover, we successfully created transgenic Dendrobium Chao Praya Smile orchids overexpressing DOAP1, which displayed earlier flowering and earlier termination of inflorescence meristems into floral meristems than wild-type orchids. Our results demonstrate that DOAP1 plays an evolutionarily conserved role in promoting flowering and floral meristem specification in the Orchidaceae family.

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Section: Introductionmentioning

confidence: 99%

DOAP1 Promotes Flowering in the Orchid Dendrobium Chao Praya Smile

et al. 2017

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“…The ap1 mutation in Arabidopsis causes the absence of petals, petal transformation into bract-like structures, and the production of axillary flowers [ 25 ]; however, its overexpression causes early flowering and transformation of shoot apical meristem into floral meristem [ 26 ]. So far, AP1 orthologs have been identified in a number of species, such as apple [ 27 ], pea [ 27 ], longan [ 28 ], common wheat [ 29 ], birch [ 30 ], trifoliate orange [ 31 ], poplar [ 32 ], and moth orchid [ 33 ]. Conserved function of AP1 orthologs in the flowering process and floral organ formation has been found in different plants.…”

Section: Introductionmentioning

confidence: 99%

Integrated proteomic, transcriptomic, and metabolomic profiling reveals that the gibberellin–abscisic acid hub runs flower development in the Chinese orchid Cymbidium sinense

Ahmad,

Lu,

Gao

et al. 2024

Horticulture Research

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The seasonal flowering Chinese Cymbidium produce axillary floral meristem and require a dormancy period during cold conditions for flower development. However, their bud activation mechanism remains elusive. This study evaluates the multi-omics across six stages of flower development, along with functional analysis of core genes to decipher the innate mechanism of floral bud initiation and outgrowth in a Chinese orchid Cymbidium sinense. The transcriptome and proteome analyses identified 10 modules with essential roles in floral bud dormancy and activation. The gene clusters in the early stages of flower development were mainly related to flowering time regulation and meristem determination, while the late stages were correlated with hormone signaling pathways. The metabolome identified 69 potential hormones, wherein GA and ABA were the main regulatory hubs, and GA4 and GA53 exhibited a reciprocal loop. Extraneous GA application caused rapid elongation of flower buds and promoted the expression of flower development genes. Contrarily, exogenous ABA application extended the dormancy process, and ABA inhibitors indorsed dormancy release. Moreover, CsAPETALA1 (CsAP1) was identified as the potential target of ABA for floral bud activation. Transformation of CsAP1 in Arabidopsis and its transient overexpression in C. sinense protoplast not only affected the flowering time and floral organ morphogenesis in Arabidopsis but also orchestrated the expression of flowering and hormone regulatory genes. Presence of ABA response elements in the CsAP1 promoter, rapid downregulation of CsAP1 after exogenous ABA application and the activation of floral bud after ABA-inhibitor treatment suggest that ABA can control the bud outgrowth through CsAP1.

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“…The functions of the AP1/FUL-like genes diverged during plant evolution. These genes have important functions in uencing plant growth and developmental processes, including oral transitions, fruit ripening, and the opening of the apical hook (Wang et al 2014a;Chen et al 2015;Li et al 2016;Führer et al 2020). In Arabidopsis, AtAP1 expressed in the early oral development and affected the positioning of organs as well as sepal and petal identity (Irish and Sussex 1990).…”

Section: Introductionmentioning

confidence: 99%

Functional Divergence of AP1 And FUL Genes Related To Flowering Regulation In Upland Cotton (Gossypium Hirsutum L.)

Zhang

Ren

et al. 2021

Preprint

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The AP1/FUL transcription factors are important for floral development, but the underlying molecular mechanisms remain unclear. In this study, we cloned and identified two AP1/FUL-like genes, GhAP1.1 and GhFUL2, in upland cotton, which is a commonly cultivated economically valuable crop. Sequence alignments and phylogenetic analyses indicated GhAP1.1 and GhFUL2, which are encoded by genes in the AP1/FUL clade, have conserved N-terminals, but diverse C-terminal domains. A quantitative real-time PCR analysis revealed that GhAP1.1 and GhFUL2 were expressed in the flower and root, and had the opposite expression patterns during different shoot apical meristem stages. The upregulated expression of GhAP1.1 in Arabidopsis and the silencing of GhAP1.1 did not induce significant changes to the flowering time or floral organs, but the transcript levels of the florigen FT gene and the AP1 homolog GhMADS42 increased. The overexpression of GhFUL2 in Arabidopsis delayed flowering and promoted bolting by decreasing the FT and LFY expression levels. Silencing GhFUL2 in cotton dramatically increased the expression of GhFT and GhMADS42 and promoted flowering. Additionally, yeast two-hybrid and bimolecular fluorescence complementation assays indicated that GhAP1.1 can interact with the SVP homolog GhSVP2.2, whereas GhFUL2 can form heterodimers with GhSEP3/GhSEP4 homologs, and GhSVP2.2. Therefore, we proved that the functional divergence of GhAP1.1 and GhFUL2, which involved changes in sequences and expression patterns, influenced the regulation of cotton flower development and plant architecture.

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Identification and expression analysis of APETALA1 homologues in poplar

Cited by 8 publications

References 56 publications

DOAP1 Promotes Flowering in the Orchid Dendrobium Chao Praya Smile

DOAP1 Promotes Flowering in the Orchid Dendrobium Chao Praya Smile

Integrated proteomic, transcriptomic, and metabolomic profiling reveals that the gibberellin–abscisic acid hub runs flower development in the Chinese orchid Cymbidium sinense

Functional Divergence of AP1 And FUL Genes Related To Flowering Regulation In Upland Cotton (Gossypium Hirsutum L.)

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