Identification and expression analysis of blue light receptor aureochrome in the harmful alga Heterosigma akashiwo (raphidophyceae)

Ji, Nanjing; Li, Ling; Lin, Lingxiao; Lin, Senjie

doi:10.1016/j.hal.2016.11.016

Cited by 9 publications

(8 citation statements)

References 71 publications

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“…Here, one of the aureochrome genes in C. antiqua ( Chaan22605 ) was shown to belong to the same clade as Haaureo1 . We cannot strictly compare the expression patterns between Chaan22605 and Haaureo1 because the light:dark cycle used by Ji et al (2017) differed from that used here. Nevertheless, we observed that the expression level of Chaan22605 was significantly higher in the daytime (roughly corresponding to T4 in Ji et al, 2017) than in the nighttime (corresponding to T12 in Ji et al, 2017) (Supplementary Data S2), which corresponds with the expression rhythm of Haaureo1 .…”

Section: Discussionmentioning

confidence: 97%

“…In addition to the aureochrome gene reported previously (Ishikawa et al, 2009), the present study newly identified five aureochrome genes in C. antiqua (Figure 2). In H. akashiwo , one of the four aureochrome genes ( Haaureo1 ) exhibits a clear diel rhythm, with the highest and the lowest transcript abundances occurring at dawn and dusk under a light:dark cycle, although the rhythm disappears under continuous dark conditions (Ji et al, 2017). Here, one of the aureochrome genes in C. antiqua ( Chaan22605 ) was shown to belong to the same clade as Haaureo1 .…”

Section: Discussionmentioning

confidence: 99%

“…Therefore, the motility can be regulated by photoreceptors that have unique spectra for absorbance and action. The gene encoding a blue light receptor aureochrome has been isolated in H. akashiwo and C. antiqua (Ishikawa et al, 2009; Ji et al, 2017), but there is little information on the genes encoding other photoreceptors such as a UV/blue light receptor, cryptochrome (CRY) and photolyase (PHR), and a red/far-red light receptor, phytochrome, which function in physiological control in various organisms (Ahmad and Cashmore, 1993; Furuya, 1993; Smith, 2000; Lin and Todo, 2005).…”

Section: Introductionmentioning

confidence: 99%

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RNA-Seq Analysis Reveals Genes Related to Photoreception, Nutrient Uptake, and Toxicity in a Noxious Red-Tide Raphidophyte Chattonella antiqua

et al. 2019

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Aquaculture industries are under threat from noxious red tides, but harm can be mitigated by precautions such as early harvesting and restricting fish feeding to just before the outbreak of a red tide. Therefore, accurate techniques for forecasting red-tide outbreaks are strongly needed. Omics analyses have the potential to expand our understanding of the eco-physiology of these organisms at the molecular level, and to facilitate identification of molecular markers for forecasting their population dynamics and occurrence of damages to fisheries. Red tides of marine raphidophytes, especially Chattonella species, often extensively harm aquaculture industries in regions with a temperate climate around the world. A red tide of Chattonella tends to develop just after an input of nutrients along the coast. Chattonella displays diurnal vertical migration regulated by a weak blue light, so it photosynthesizes in the surface layer during the daytime and takes up nutrients in the bottom layer during the nighttime. Superoxide produced by Chattonella cells is a strong candidate for the cause of its toxicity to bacteria and fishes. Here we conducted mRNA-seq of Chattonella antiqua to identify genes with functions closely related to the dynamics of the noxious red tide, such as photosynthesis, photoreception, nutrient uptake, and superoxide production. The genes related to photosynthetic pigment biosynthesis and nutrient uptake had high similarity with those of model organisms of plants and algae and other red-tide microalgae. We identified orthologous genes of photoreceptors such as aureochrome (newly five genes), the cryptochrome/photolyase (CRY/PHR) family (6-4PHR, plant CRY or cyclobutane pyrimidine dimer [CPD] Class III, CPD Class II, and CRY-DASH), and phytochrome (four genes), which regulate various physiological processes such as flagellar motion and cell cycle in model organisms. Six orthologous genes of NADPH oxidase, which produces superoxide on the cell membrane, were found and divided into two types: one with 5–6 transmembrane domains and another with 11 transmembrane domains. The present study should open the way for analyzing the eco-physiological features of marine raphidophytes at the molecular level.

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Section: Discussionmentioning

confidence: 97%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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RNA-Seq Analysis Reveals Genes Related to Photoreception, Nutrient Uptake, and Toxicity in a Noxious Red-Tide Raphidophyte Chattonella antiqua

et al. 2019

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“…In the single cellular diatom Phaeodactylum tricornutum, PtAUREO1a and PtAUREO1b mutants exhibit signi cantly decreased photoacclimation to blue light [11,12]. AUREO1 in Heterosigma akashiwois may act as a blue light-sensitive negative gene regulator, which is driven by dark-to-light transitional cues [13]. Although the function of aureochrome has been veri ed in various species, further details regarding the transcription regulation of aureochrome remain to be further explored.…”

Section: Introductionmentioning

confidence: 99%

miR8181 is Involved in the Cell Growth and Development Regulation of Saccharina Japonica

Yang

Wang

Jian

et al. 2020

Preprint

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Background: Aureochrome, a blue-light receptor found in photosynthetic stramenopiles, plays an important role in brown algal growth and development. Aureochrome preserves the reversed effector-sensor domain for blue light reception and acts as the candidate optogenetic tool for light induced post-transcriptional regulation, but the inner rapid regulation of aureochrome remains to be studied. MicroRNA (miRNAs) of plant can cleavage the specific base-pairing site of mRNA by RNA interference mechanism, and such post-transcriptional regulation of miRNAs to photoreceptor has received attention due to the flexible regulation pathway. However, the targeting relationship between aureochrome and miRNA is unclear.Results: In this study, the potential regulatory network between miRNAs and aureochrome were explored by transcriptome and sRNA sequencing in Saccharina japonica. Our results found that 18 miRNAs perfectly paired with aureochrome. Among the screened miRNAs, miR8181-x was negatively correlated with aureochrome5 with high credibility and exhibited tissue-specific expression in S. japonica. Degradome sequencing detected the exact cleavage site of miR8181-x on aureochrome5, confirming their targeting regulation relationship. Among the 54 target genes of miR8181-x, nine genes of ABC transporters, E3 ubiquitin-protein protein ligase, Hsp90, Mx1, PetC, EF2, GSA, HAD-superfamily hydrolase and SET2 that exhibited similar expression with aureochrome5 competed with the same binding site, thus constructing the competing endogenous RNA network. Functional analysis of miR8181-x target genes revealed that regulation of cell differentiation and development was enriched, indicating the potential role of miR8181-x in the regulation of growth and development.Conclusion: Our study found that miR8181-x negatively regulated the expression of aureochrome5. The exact cleavage site in aureochrome5 were verified by degradome sequencing, confirming the targeting relationships. Functional enrichment of miR8181-x target genes revealed that miR8181-x involved in the cell growth and development regulation of S. japonica.

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“…In this review, we focus on the distribution, the structural response, as well as the functional characterization of aureochromes, light driven transcription factors that just recently have been discovered (Takahashi et al, 2007) in some stramenopiles, including brown algae (Ishikawa et al, 2009), diatoms (Bowler et al, 2008), raphidophytes (Jia et al, 2017), and eustigmatophytes (Vieler et al, 2012).…”

Section: Introductionmentioning

confidence: 99%

An update on aureochromes: Phylogeny – mechanism – function

Kroth

Wilhelm

Kottke

2017

Journal of Plant Physiology

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Light is important for algae, as it warrants metabolic independence via photosynthesis. In addition to the absorption of light by the photosystems, algae possess a variety of specific photoreceptors that allow the quantification of the light fluxes as well as the assessment of light qualities. About a decade ago, aureochromes have been described in the xanthophyte alga Vaucheria frigida. These proteins represent a new type of blue light photoreceptor as they possess both a light-oxygen-voltage (LOV) domain for light reception as well as a basic region leucine zipper (bZIP) domain for DNA binding, indicating that they represent light-driven transcription factors. Aureochromes so far have been detected only in a single group of algae, photosynthetic stramenopiles, but not in any other prokaryotic or eukaryotic organisms. Recent biophysical work on aureochromes in the absence and the presence of DNA revealed the mechanism of allosteric communication between the sensor and effector domains despite their unusual inversed arrangement. Different molecular models have been proposed to describe the effect of light on DNA binding. Functional characterization of mutants of the diatom Phaeodactylum tricornutum, in which the aureochrome genes have been silenced or deleted, indicate that different aureochromes may have different functions, being involved in central processes like light acclimation and regulation of the cell cycle.

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Identification and expression analysis of blue light receptor aureochrome in the harmful alga Heterosigma akashiwo (raphidophyceae)

Cited by 9 publications

References 71 publications

RNA-Seq Analysis Reveals Genes Related to Photoreception, Nutrient Uptake, and Toxicity in a Noxious Red-Tide Raphidophyte Chattonella antiqua

RNA-Seq Analysis Reveals Genes Related to Photoreception, Nutrient Uptake, and Toxicity in a Noxious Red-Tide Raphidophyte Chattonella antiqua

miR8181 is Involved in the Cell Growth and Development Regulation of Saccharina Japonica

An update on aureochromes: Phylogeny – mechanism – function

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Identification and expression analysis of blue light receptor aureochrome in the harmful alga Heterosigma akashiwo (raphidophyceae)

Cited by 9 publications

References 71 publications

RNA-Seq Analysis Reveals Genes Related to Photoreception, Nutrient Uptake, and Toxicity in a Noxious Red-Tide Raphidophyte Chattonella antiqua

RNA-Seq Analysis Reveals Genes Related to Photoreception, Nutrient Uptake, and Toxicity in a Noxious Red-Tide Raphidophyte Chattonella antiqua

miR8181&nbsp;is Involved in the&nbsp;Cell Growth and Development Regulation of&nbsp;Saccharina Japonica

An update on aureochromes: Phylogeny – mechanism – function

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miR8181 is Involved in the Cell Growth and Development Regulation of Saccharina Japonica