Identification and expression analysis of the IPT and CKX gene families during axillary bud outgrowth in apple ( Malus domestica Borkh.)

Tan, Ming; Li, Guofang; Qi, Shihua; Liu, Xiaojie; Chen, Xilong; Ma, Junsheng; Zhang, Dong; Han, Mingyu

doi:10.1016/j.gene.2018.01.101

Cited by 50 publications

(48 citation statements)

References 66 publications

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“…In Arabidopsis, IPT single or multiple mutants exhibited significant reductions in branching [50]. According to the researchers, it is possible that the primary endogenous cytokinin synthesized mainly by adenylate IPTs plays a greater functional role in plant development and is more highly regulated [47,51]. In contrast, most of the CKX genes were downregulated in HB-E, HB-G1 and HB-G2 ( Fig.…”

Section: Cytokinin and Gibberellin Are Important Regulators Of Axillamentioning

confidence: 97%

“…Many of the differentially expressed genes between Z571 and mZ571 related to the axillary bud type coincided with recent reports. In particular, an miaA gene (LOC107903664) and one IPT gene (LOC107912422) that were upregulated in apple [18,47] were also found to be upregulated in mZ571. In fact, recent studies have explored the functions of IPT and CKX genes in various plant species.…”

Section: Cytokinin and Gibberellin Are Important Regulators Of Axillamentioning

confidence: 98%

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Transcriptome analyses provide insights into the homeostatic regulation of axillary buds in upland cotton (G. hirsutum L.)

et al. 2020

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Background: The axillary bud is an important index of cotton plant-type traits, and the molecular mechanism of axillary bud development in upland cotton has not yet been reported. We obtained a mutant (designated mZ571) with a high-budding phenotype in axillary bud development from the low-budding phenotype variety G. hirsutum Z571 (CCRI 9A02), which provided ideal materials for the study of complex regulatory networks of axillary bud development. In this study, RNA sequencing was carried out to detect gene expression levels during three stages of axillary buds in Z571 (LB, low budding) and mZ571 mutant (HB, high budding). Results: A total of 7162 DEGs were identified in the three groups (HB-E vs. LB-E, HB-G1 vs. LB-G1, HB-G2 vs. LB-G2), including 4014 downregulated and 3184 upregulated DEGs. Additionally, 221 DEGs were commonly identified in all three groups, accounting for approximately 3.09% of the total DEGs. These DEGs were identified, annotated and classified. A significant number of DEGs were related to hormone metabolism, hormone signal transduction, and starch and sucrose metabolism. In addition, 45, 22 and 9 DEGs involved in hormone metabolic pathways and 67, 22 and 19 DEGs involved in hormone signal transduction pathwayspathway were identified in HB-E vs. LB-E, HB-G1 vs. LB-G1, and HB-G2 vs. LB-G2, respectively, suggesting that endogenous hormones are the primary factors influencing cotton axillary bud growth. Hormone and soluble sugar content measurements revealed that mZ571 exhibited higher concentrations of zeatin, gibberellins and soluble sugar in all three stages, which confirmed that these hormone metabolism-, hormone signal transduction-and starch metabolism-related genes showed interaction effects contributing to the divergence of axillary bud growth between mZ571 and Z571. Conclusions: Our results confirmed the importance of endogenous hormones and sugars in the development of axillary buds, and we found that mZ571 plants, with a high-budding phenotype of axillary buds, exhibited higher endogenous hormone and sugar concentrations. Overall, we present a model for the emergence and development of cotton axillary buds that provides insights into the complexity and dynamic nature of the regulatory network during axillary bud emergence and development.

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Section: Cytokinin and Gibberellin Are Important Regulators Of Axillamentioning

confidence: 97%

Section: Cytokinin and Gibberellin Are Important Regulators Of Axillamentioning

confidence: 98%

Transcriptome analyses provide insights into the homeostatic regulation of axillary buds in upland cotton (G. hirsutum L.)

et al. 2020

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“…Among those GFMs, AtIPT3 and AtPT9 expression was down regulated under drought conditions [64]. Since the application of whole-genome sequencing to a wider range of plant species, the IPT family has been successfully identified and annotated, with 9 members in maize [35], 14 members in soybean (Glycine max) [65], 10 members in rice (Oryza sativa) [66], and 12 members in apple (Malus domestica) [67]. An increasing number of investigations focus on the participation of IPT genes in the regulation of plant defense response to water deficit in crop species.…”

Section: Response Of Ck Metabolic Genes To Droughtmentioning

confidence: 99%

“…The other important component of CK metabolism is the CKX enzyme, which accounts for the irreversible inactivation of CKs by cleaving the side chain from CK molecules [46]. Genome-wide association studies have recently shed more light on the potential function of CKX members in plant species, with 13 CKX GFMs in maize [71], 11 CKX GFMs in rice [66], 12 CKX GFMs in apple [67], CKX GFMs in wheat (Triticum aestivum) [72] and 23 CKX GFMs in rapeseed (Brassica napus) [73]. Increasing lines of evidence suggest that CKX GFMs play important roles in various plant physiological and developmental modifications under drought stress [74][75][76] (Table 1).…”

Section: Response Of Ck Metabolic Genes To Droughtmentioning

confidence: 99%

Role and Regulation of Cytokinins in Plant Response to Drought Stress

Hải

Chuong

et al. 2020

Plants

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Cytokinins (CKs) are key phytohormones that not only regulate plant growth and development but also mediate plant tolerance to drought stress. Recent advances in genome-wide association studies coupled with in planta characterization have opened new avenues to investigate the drought-responsive expression of CK metabolic and signaling genes, as well as their functions in plant adaptation to drought. Under water deficit, CK signaling has evolved as an inter-cellular communication network which is essential to crosstalk with other types of phytohormones and their regulating pathways in mediating plant stress response. In this review, we revise the current understanding of CK involvement in drought stress tolerance. Particularly, a genetic framework for CK signaling and CK crosstalk with abscisic acid (ABA) in the precise monitoring of drought responses is proposed. In addition, the potential of endogenous CK alteration in crops towards developing drought-tolerant crops is also discussed.

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“…S18). 30,[37][38][39][40] After pretreated with lovastatin or colchicine for one day to deplete endogenous cytokinins or to reduce cell division activities, the seedlings were then transferred to hydrostimulating medium containing either lovastatin or colchicine. Interestingly, with the presence of any one of the inhibitors, root hydrotropism was completely diminished, although roots were still able to elongate possibly due to cell expansion (Supplementary information, Fig.…”

Section: Asymmetric Distribution Of Cytokinins Can Cause Unequal Cellmentioning

confidence: 99%

Asymmetric distribution of cytokinins determines root hydrotropism in Arabidopsis thaliana

Chang

et al. 2019

Cell Res

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The phenomenon of plant root tips sensing moisture gradient in soil and growing towards higher water potential is designated as root hydrotropism, which is critical for plants to survive when water is a limited factor. Molecular mechanisms regulating such a fundamental process, however, are largely unknown. Here we report our identification that cytokinins are key signaling molecules directing root growth orientation in a hydrostimulation (moisture gradient) condition. Lower water potential side of the root tip shows more cytokinin response relative to the higher water potential side. Consequently, two cytokinin downstream type-A response regulators, ARR16 and ARR17, were found to be up-regulated at the lower water potential side, causing increased cell division in the meristem zone, which allows the root to bend towards higher water potential side. Genetic analyses indicated that various cytokinin biosynthesis and signaling mutants, including the arr16 arr17 double mutant, are significantly less responsive to hydrostimulation. Consistently, treatments with chemical inhibitors interfering with either cytokinin biosynthesis or cell division completely abolished root hydrotropic response. Asymmetrically induced expression of ARR16 or ARR17 effectively led to root bending in both wild-type and miz1, a previously known hydrotropism-defective mutant. These data demonstrate that asymmetric cytokinin distribution is a primary determinant governing root hydrotropism.Cell Research (2019) 29:984-993; https://doi.

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Identification and expression analysis of the IPT and CKX gene families during axillary bud outgrowth in apple ( Malus domestica Borkh.)

Cited by 50 publications

References 66 publications

Transcriptome analyses provide insights into the homeostatic regulation of axillary buds in upland cotton (G. hirsutum L.)

Transcriptome analyses provide insights into the homeostatic regulation of axillary buds in upland cotton (G. hirsutum L.)

Role and Regulation of Cytokinins in Plant Response to Drought Stress

Asymmetric distribution of cytokinins determines root hydrotropism in Arabidopsis thaliana

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