Identification and expression analysis of major histocompatibility complex IIB gene in orange‐spotted grouper <i>Epinephelus coioides</i>

Lu, Danqi; Yi, Shibai; Yao, Mi; Li, Y. W.; Liu, Xiaochun; Zhang, Y.; Lin, Haoran

doi:10.1111/j.1095-8649.2012.03321.x

Cited by 6 publications

(5 citation statements)

References 46 publications

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“…However, the expression levels of MHC-II were not significantly different among each group before and after L. crocea was immunized with rCiSA32.6t in this work, which is in agreement with earlier reports on inactivate theront-immunized E. coioids (Dan et al 2013 ). It is speculated that MHC-II may not be involved in the presentation of the C. irritans surface antigen CiSA32.6t or MHC-II antigen presentation may occur at an early stage (1 to 7 days) after immunization with C. irritans vaccine (Deng et al 2020 ; Lu et al 2012 ). IgM is discovered to function as the front-line molecules of host defense against pathogen infection and plays an important role in humoral immunity by binding antigens and activating the complement system (Mashoof and Criscitiello 2016 ).…”

Section: Discussionmentioning

confidence: 99%

Immune response of large yellow croaker Larimichthys crocea towards a recombinant vaccine candidate targeting the parasitic ciliate Cryptocaryon irritans

Jiang

Huang

et al. 2023

Aquacult Int

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Cryptocaryon irritans , a parasitic ciliate, pose a major threat to marine teleost fish aquaculture. So far, no effective and safe control method is available. In this study, the protective efficacy of a recombinant truncated surface antigen of C. irritans (rCiSA32.6t) for large yellow croaker ( Larimichthys crocea ) against the parasite challenge with a sub-lethal dose of the infective theronts was evaluated by comparing the relative percent survivals (RPS), the specific antibody titers in sera, and the expression levels of the immune-related genes among the negative or adjuvant control fish, fish intraperitoneally immunized with rCiSA32.6t. The results showed that a RPS of 50.1% in rCiSA32.6t-immunized fish was achieved in comparison to negative control fish against C. irritans . A significant increase was noted in the antigen-specific immunoglobulin M (IgM) and immunoglobulin T (IgT) antibody levels in the sera of the rCiSA32.6t-vaccinated fish. Compared to the negative control fish, quantitative real-time PCR analysis indicated that the interleukin-1beta, IgT, and IgM heavy chain mRNA level in the fish head kidney, spleen, gill, and skin tissue were upregulated post-rCiSA32.6t immunization. This study indicates that the rCiSA32.6t can provide a high level of immune protection against C. irritans infection in grouper and is therefore pursued as a candidate C. irritans vaccine.

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Section: Discussionmentioning

confidence: 99%

Immune response of large yellow croaker Larimichthys crocea towards a recombinant vaccine candidate targeting the parasitic ciliate Cryptocaryon irritans

Jiang

Huang

et al. 2023

Aquacult Int

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“…coioides was sharply down‐regulated in the head kidney, spleen and gills from day 1 to day 3 post C . irritans infection (Lu et al , ).…”

Section: Discussionmentioning

confidence: 99%

Co‐expression of march5b and tlr7 in large yellow croaker Larimichthys crocea in response to Cryptocaryon irritans infection

Zhang

Chen

et al. 2015

Journal of Fish Biology

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In this study, molecular characteristics of march5b and co-expression of march5b and tlr7 in response to the infection of Cryptocaryon irritans in the large yellow croaker Larimichthys crocea were investigated. The full-length complementary (c)DNA of march5b was 1314 bp, including an open reading frame of 846 bp encoding a polypeptide of 281 amino acids, and the full-length genomic sequence was composed of 23,577 nucleotides, including six exons and five introns. The putative March5b protein contained a RINGv motif and four transmembrane domains. The march5b transcripts were broadly distributed in all detected tissues, with a strong expression in blood, brain and gills, and a weak expression in kidney by quantitative PCR analysis. The expression of march5b and tlr7 in the skin, gills, spleen and head kidney changed in the same manner at most time points post-primary infection with C. irritans. Significant increase was observed in the skin with march5b at days 2 and 3 by 26.10 and 6.88 fold, respectively, and with tlr7 at day 3 by 57.68 fold, when compared with the control. Their expressions, however, were decreased in the gills, especially at day 3 (march5b by 8.9%, tlr7 by 22.06%). In the spleen and head kidney, march5b and tlr7 transcripts were up-regulated early, then noticeably declined at day 3. These results suggested that march5b and tlr7 are co-expressed in response to parasite infection and March5b probably catalyses ubiquitination of some proteins of TLR7 signalling pathway.

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“…Primary spleen cells from T. nigroviridis were obtained as previously described, with minor modifications [50]. Briefly, tissue culture medium (TCM) was prepared from RPMI-1640 medium (Life Technologies, Gaithersburg, MD, USA) by adding 10% fetal bovine serum (Life Technologies), 2 mM L-glutamine (Sigma, St. Louis, MO, USA), and 1% penicillin/streptomycin (Sigma).…”

Section: Methodsmentioning

confidence: 99%

Differential Expression Profiling of Spleen MicroRNAs in Response to Two Distinct Type II Interferons in Tetraodon nigroviridis

Wan

et al. 2014

PLoS ONE

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MicroRNAs are endogenous, small non-coding RNAs approximately 18–26 nucleotides in length that regulate target gene expression at the post-transcription level. Interferon-γ (IFN-γ) is a Th1 cytokine that is involved in both the innate and adaptive immune responses. We previously identified two IFN-γ genes in green-spotted puffer fish (Tetraodon nigroviridis). To determine whether miRNAs participate in IFN-γ-related immune responses, T. nigroviridis spleen cells were treated with recombinant IFN-γ isoforms, and a Solexa high-throughput sequencing method was used to identify miRNAs. In total, 1,556, 1,538 and 1,573 miRNAs were found in the three samples, and differentially expressed miRNAs were determined. In total, 398 miRNAs were differentially expressed after rIFN-γ1 treatment, and 438 miRNAs were differentially expressed after rIFN-γ2 treatment; additionally, 403 miRNAs were differentially expressed between the treatment groups. Ten differentially expressed miRNAs were chosen for validation using qRT-PCR. Target genes for the differentially expressed miRNAs were predicted, and GO and KEGG analyses were performed. This study provides basic knowledge regarding fish IFN-γ-induced miRNAs and offers clues for further studies into the mechanisms underlying fish IFN-γ-mediated immune responses.

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Identification and expression analysis of major histocompatibility complex IIB gene in orange‐spotted grouper Epinephelus coioides

Cited by 6 publications

References 46 publications

Immune response of large yellow croaker Larimichthys crocea towards a recombinant vaccine candidate targeting the parasitic ciliate Cryptocaryon irritans

Immune response of large yellow croaker Larimichthys crocea towards a recombinant vaccine candidate targeting the parasitic ciliate Cryptocaryon irritans

Co‐expression of march5b and tlr7 in large yellow croaker Larimichthys crocea in response to Cryptocaryon irritans infection

Differential Expression Profiling of Spleen MicroRNAs in Response to Two Distinct Type II Interferons in Tetraodon nigroviridis

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