Identification and evaluation of the probiotic potential of lactobacilli isolated from canine milk

Martı́n, Rocı́o; Olivares, Mónica; Pérez, M.A. Ferrús; Xaus, Jordi; Torre, Celina; Fernández, Leónides; Rodríguez, Juan M.

doi:10.1016/j.tvjl.2009.04.014

Cited by 40 publications

(35 citation statements)

References 46 publications

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“…The KAA counts (<1.16 × 10 3 CFU/mL) were similar to those reported for hygienically-obtained human milk on MRS plates, a medium also suitable for isolation of enterococci [6,7]. As previously reported for lactobacilli in porcine and canine milk [8,9], the enterococcal pattern observed in the milk samples seems to be restricted to a low number of species and strains, and also to have a high degree of individual variability. To our knowledge, this is the first description of enterococci isolated from fresh milk of healthy canine, feline and porcine hosts.…”

Section: Discussionsupporting

confidence: 84%

Antibiotic resistance, virulence determinants and production of biogenic amines among enterococci from ovine, feline, canine, porcine and human milk

et al. 2013

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BackgroundRecent studies have shown that mammalian milk represents a continuous supply of commensal bacteria, including enterococci. The objectives of this study were to evaluate the presence of enterococci in milk of different species and to screen them for several genetic and phenotypic traits of clinical significance among enterococci.ResultsSamples were obtained from, at least, nine porcine, canine, ovine, feline and human healthy hosts. Enterococci could be isolated, at a concentration of 1.00 × 102 -1.16 × 103 CFU/ml, from all the porcine samples and, also from 85, 50, 25 and 25% of the human, canine, feline and ovine ones, respectively. They were identified as Enterococcus faecalis, Enterococcus faecium, Enterococcus hirae, Enterococcus casseliflavus and Enterococcus durans. Among the 120 initial enterococcal isolates, 36 were selected on the basis of their different PFGE profiles and further characterized. MLST analysis revealed a wide diversity of STs among the E. faecalis and E. faecium strains, including some frequently associated to hospital infections and novel STs. All the E. faecalis strains possessed some of the potential virulence determinants (cad, ccf, cob, cpd, efaAfs, agg2, gelE, cylA, espfs) assayed while the E. faecium ones only harboured the efaAfm gene. All the tested strains were susceptible to tigecycline, linezolid and vancomycin, and produced tyramine. Their susceptibility to the rest of the antimicrobials and their ability to produce other biogenic amines varied depending on the strain. Enterococci strains isolated from porcine samples showed the widest spectrum of antibiotic resistance.ConclusionsEnterococci isolated from milk of different mammals showed a great genetic diversity. The wide distribution of virulence genes and/or antibiotic resistance among the E. faecalis and E. faecium isolates indicates that they can constitute a reservoir of such traits and a risk to animal and human health.

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Section: Discussionsupporting

confidence: 84%

Antibiotic resistance, virulence determinants and production of biogenic amines among enterococci from ovine, feline, canine, porcine and human milk

et al. 2013

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“…above 15 mm (+++) were observed in a total of 6 isolates (L8, L12, L15, L16, L18 and L38) against 9 out of 11 test pathogens examined, 4 isolates (L13, L26, L33 and L42) against 5 out of 11 test pathogens examined, one isolate (L6) against 6 out of 11 test pathogens examined, one isolate (L2) against 5 out of 11 test pathogens examined, 5 isolates (L11, L14, L17, L35 and L41) against 1 out of 11 test pathogens examined, whereas in 3 isolates (L20, L31 and L36) inhibition zones above 15 mm (+++) were not observed (Table 1). The differences among antimicrobial activities displayed by different strains tested were supposed to be due to differences in production of organic acids like lactic acid or proteinaceous inhibitory substances (Martin et al, 2010). Antibacterial activity was found to be prominent (with inhibition zones above 15 mm) against O49, O128 and rough (R) serotypes of E. coli compared to O20 and O141 E. coli serotypes (Table 1).…”

Section: Resultsmentioning

confidence: 99%

Modeling lactation curve for genetic evaluation of Murrah buffaloes

Sahoo

Singh

Dash

et al. 2017

IJAR

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The present study aimed at the detection of suitable Lactobacillus species from dogs for usage as probiotic. A total of 67 rectal swabs from healthy pups were analyzed and 49 (73.1%) Lactobacillus isolates were identified based on morphological, biochemical characteristics and confirmed by genus specific PCR targeting 16S rRNA gene. A total of 20 isolates that showed strong aggregation, high cell surface hydrophobicity, acid and bile tolerance were screened for in-vitro antibacterial activity by agar well diffusion assay, where prominent inhibitory zones were observed against majority of the test pathogens. Reduction in antibacterial activity was noticed after neutralization, proteinase K and heat treatment of supernatants. Partial 16S rRNA nucleotide sequence analysis of seven Lactobacillus isolates that showed prominent in-vitro antibacterial activity revealed maximum sequence homology with Lactobacillus fermentum (for six isolates) and Lactobacillus agilis (for one isolate). The in-vitro antibacterial activity results highlighted the need for in-vivo studies in India to establish probiotic potential of canine faecal Lactobacillus species in the near future.

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“…L. reuteri has been experimentally shown to be maternally transmitted in humans and pigs (63,64). Interestingly, L. reuteri is present in low numbers in milk from humans, pigs, and dogs, and thus transmission to the next generation might be facilitated by inoculation during lactation (65)(66)(67).…”

Section: Lactobacillus Reuteri As a Model Gut Symbiontmentioning

confidence: 99%

Host-microbial symbiosis in the vertebrate gastrointestinal tract and the Lactobacillus reuteri paradigm

Walter

Britton

Roos

2010

Proc. Natl. Acad. Sci. U.S.A.

282

279

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Vertebrates engage in symbiotic associations with vast and complex microbial communities that colonize their gastrointestinal tracts. Recent advances have provided mechanistic insight into the important contributions of the gut microbiome to vertebrate biology, but questions remain about the evolutionary processes that have shaped symbiotic interactions in the gut and the consequences that arise for both the microbes and the host. Here we discuss the biological principles that underlie microbial symbiosis in the vertebrate gut and the potential of the development of mutualism. We then review phylogenetic and experimental studies on the vertebrate symbiont Lactobacillus reuteri that have provided novel insight into the ecological and evolutionary strategy of a gut microbe and its relationship with the host. We argue that a mechanistic understanding of the microbial symbiosis in the vertebrate gut and its evolution will be important to determine how this relationship can go awry, and it may reveal possibilities by which the gut microbiome can be manipulated to support health.vertebrate symbiont | microbiota | mutualism

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Identification and evaluation of the probiotic potential of lactobacilli isolated from canine milk

Cited by 40 publications

References 46 publications

Antibiotic resistance, virulence determinants and production of biogenic amines among enterococci from ovine, feline, canine, porcine and human milk

Antibiotic resistance, virulence determinants and production of biogenic amines among enterococci from ovine, feline, canine, porcine and human milk

Modeling lactation curve for genetic evaluation of Murrah buffaloes

Host-microbial symbiosis in the vertebrate gastrointestinal tract and the Lactobacillus reuteri paradigm

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