Identification And Evaluation of Reference Genes For Cynanchum Auriculatum Under Various Stress Conditions

Abstract

Baishouwu (Cynanchum auriculatum) is a kind of critical Chinese herbal medicine. However, compared with the studies of other Chinese herbal medicines, the screening study on the reference genes of C. auriculatum is still the blank. Deterioration of the natural environment severely affects the growth and development of C. auriculatum. This study screened and identified suitable reference genes of C. auriculatum under various stress conditions. Based on qRT-PCR, geNorm, NormFinder, BestKeeper, and RefFinder were used for the expression stability evaluation of 12 potential reference genes from C. auriculatum. The ranking table showed that optimal reference genes included EF2 and SAMDC (heat stress), CYP and TUB-β (cold stress), TUB-α and GAPDH (drought stress), SAMDC and TUB-α (waterlogging stress), along with EF2 and ACT7 (salt stress). These results also demonstrated that under different abiotic stresses, suitable reference genes of plants should be selected for qRT-PCR analysis.

Transcriptome-wide identification of WRKY transcription factors and their expression profiles under different stress in Cynanchum thesioides

“…Relative expression levels were calculated using the 2 −ΔΔCT method ( Livak & Schmittgen, 2001 ). By screening four reference genes of Cynanchum auriculatum, we found that ACT7 was the most stable under different abiotic stresses and hormone treatments and that GAPDH was the most stable in different tissues, so we selected ACT7 and GAPDH as internal control ( Zhu et al, 2022 ).…”