Identification and Estrogen Induction of Two Estrogen Receptors (ER) Messenger Ribonucleic Acids in the Rainbow Trout Liver: Sequence Homology with other ERs

Pakdel, Farzad; Guellec, Catherine Le; Vaillant, Colette; Roux, Marie Gaëlle Le; Valotaire, Yves

doi:10.1210/mend-3-1-44

Cited by 170 publications

(79 citation statements)

References 29 publications

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“…The rtER cDNA insert (Pakdel et al, 1989) and the Vg cDNA insert (Le were labelled with the random primer labelling kit (Amersham), in the presence of [ cr-32P]dCTP ( -800 Ci/mmol).…”

Section: Labelling Of Cdna Probementioning

confidence: 99%

In vivo estrogen induction of hepatic estrogen receptor mRNA and correlation with vitellogenin mRNA in rainbow trout

Pakdel

Féon

Gac

et al. 1991

Molecular and Cellular Endocrinology

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160

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SummaryWe have previously described the cloning, sequencing and in vitro expression of a full-length rainbow trout estrogen receptor cDNA (rtER cDNA). This full cDNA randomly labelled was used to study the estrogen induction of hepatic rtER mRNA in correlation with vitellogenin (Vg) mRNA in different physiolo~cal situations.In this paper, we show that in the liver two mRNA species are under hormonal control and their level increases about g-fold after estrogen stimulation.These two mRNAs are expressed and induced in the liver as early as the hatching stage in correlation with the expression of Vg mRNA. A long-term analysis of rtER mRNA after estradiol (E2) injection shows a transient induction of the nuclear ER and its mRNA which recover to the basal level after 2 weeks. Nevertheless, a memory effect was observed on the expression of the Vg gene which does not appear to be directly related to the estrogen receptor level.

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“…The rtER cDNA insert (Pakdel et al, 1989) and the Vg cDNA insert (Le were labelled with the random primer labelling kit (Amersham), in the presence of [ cr-32P]dCTP ( -800 Ci/mmol).…”

Section: Labelling Of Cdna Probementioning

confidence: 99%

In vivo estrogen induction of hepatic estrogen receptor mRNA and correlation with vitellogenin mRNA in rainbow trout

Pakdel

Féon

Gac

et al. 1991

Molecular and Cellular Endocrinology

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160

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“…Hybridization was performed under high stringency conditions (Thomas, 1980) using rainbow trout PRL cDNA . The nylon membrane (BiodynPall) was then stripped and reprobed for p-actin mRNA using rainbow trout cDNA (Pakdel et al, 1989). The autoradiographs were quantified by densitometric scanning.…”

Section: Prl Mrh!a Measurementmentioning

confidence: 99%

“…Hybridization was performed with a 32P-labeled complete rtER cDNA under Thomas' conditions (1980). Trout pro-opiomelanocortin (POMC) ) and rtER (Pakdel et al, 1989) cDNAs were cloned in our laboratory. Full-length rtER cDNA as well as two different restriction fragments, PstI-PstI encoding the C and D domains and ApaI-Apal encoding E domain of rtER, were used in hybridization experiments.…”

Section: Northern Blot Analysismentioning

confidence: 99%

“…This was completed by localization of estradiol receptor (ER) in the pituitary, in particular with regard to PRL cells which are located in the rostra1 part of the adenohypophysis. Using a cDNA probe coding for rainbow trout estradiol receptor (rtER) (Pakdel et al, 1989), Northern blot and in situ hybridization analyses of different parts of the pituitary were performed.…”

Section: Introductionmentioning

confidence: 99%

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Absence of direct regulation of prolactin cells by estradiol-17β in rainbow trout (Oncorhynchus mykiss)

Goff

Salbert

Prunet

et al. 1992

Molecular and Cellular Endocrinology

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SummaryThe effects of estradiol-17/3 (E,) implants on plasma prolactin (PRL) concentrations, pituitary PRL content and pituitary PRL mRNA levels were examined in rainbow trout (Oncorhynchus mykiss). Intact immature fish treated with 1 mg estradiol-17/? did not show significant changes in both PRL mRNA levels and pituitary PRL content after 3 days of treatment. In a similar experiment, no changes were observed in plasma PRL levels followed during 7 days. Similarly, lack of estradiol-17/3 effect on plasma PRL levels and on final PRL pituitary content was observed in ovariectomized female rainbow trout treated during 48 days with 25 mg estradiol-17P and in mature male fish over a 3-week treatment period. Localization of estradiol receptor (ER) mRNAs in the pituitary was carried out by Northern blot analysis using a full-length rainbow trout estrogen receptor (rtER) cDNA as a probe. The rostra1 pars distalis of the pituitary which contained mostly PRL cells showed the lower amount of rtER mRNA when compared to other parts of the pituitary. Moreover, two mRNAs of different size (3.5 and 1.4 kb) were detected in different parts of the pituitary. Further hybridization experiments using probes containing part of the rtER cDNA (E domain or C and D domains) indicated that the small-sized mRNA (1.4 kb) probably encodes a truncated ER protein lacking hormone binding domain or an ER-related protein. Thus, only the 3.56 kb mRNA appeared to be involved in the regulation of pituitary function by estradiol. In situ hybridization analysis allowed a more precise localization of this rtER mRNA in the pituitary. No labeling was discernible over PRL cells whereas other parts of the pituitary were labeled. Grain counting corroborated this localization. These results indicate that (1) in vivo estradiol-17P treatment did not modify PRL cell activity in rainbow trout, (2) PRL secretion, in this fish species, is not directly regulated by estradiol.

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“…From the work of Saceda et al (57), it appears that estradiol (E2) can upregulate the ER gene activity in MCF-7 cells, but the DNA sequences mediating this effect are still unknown. In several oviparous species and in rat, such an autoregulation has been shown in liver cells, where estrogen exert a positive control on ER mRNA and/or protein levels (48,49,61,65). We have recently demonstrated that in rainbow trout, upregulation is mediated by two distinct mechanisms: estrogen induces both a stabilization of the rainbow trout ER (rtER) mRNA and an increase in the rtER gene Cell culture and transient transfection.…”

mentioning

confidence: 99%

The Nuclear Orphan Receptors COUP-TF and ARP-1 Positively Regulate the Trout Estrogen Receptor Gene through Enhancing Autoregulation

Lazennec

Kern

Valotaire

et al. 1997

Molecular and Cellular Biology

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The rainbow trout estrogen receptor (rtER) is a positively autoregulated gene in liver cells. In a previous report, we showed that upregulation is mediated by an estrogen response element (ERE) located in the proximal promoter of the gene and that a half binding site for nuclear receptors (5-TGACCT-3) located 15 bp upstream of the ERE is involved in the magnitude of the estrogen response. We now report that the human orphan receptor COUP-TF and a COUP-TF-like protein from trout liver are able to bind to the consensus half-site. When cotransfected with the rtER gene proximal promoter, COUP-TF had no regulatory functions on its own. Interestingly, COUP-TF enhanced rtER transactivation properties in the presence of estradiol in a dose-dependent manner when cotransfected with the rtER gene promoter. Unliganded retinoid receptor heterodimers had the same helper function as COUP-TF in the presence of estradiol but were switched to repressors when the ligand all-trans-retinoic acid was added. Mutation of the consensus half-site only slightly reduced COUP-TF helper function, suggesting that it actually results from a complex mechanism that probably involves both DNA binding of COUP-TF to the promoter and protein-protein interaction with another transcription factor bound to the promoter. Nevertheless, a DNA-binding-defective mutant of COUP-TF was also defective in ER helper function. Competition footprinting analysis suggested that COUP-TF actually establishes contacts with the consensus upstream half-site and the downstream ERE half-site that would form a DR-24-like response element. Interaction of COUP-TF with the DR-24 element was confirmed in footprinting assays by using nuclear extracts from Saccharomyces cerevisiae expressing COUP-TF. Finally, interaction of COUP-TF with mutants of the rtER gene promoter showed that COUP-TF recognizes the ERE when the upstream half-site is mutated. These data show that COUP-TF may activate transcription through interaction with other nuclear receptors. This cross-talk between liganded nuclear receptors and orphan receptors is likely to modulate the spectrum of action of a particular ligand-receptor complex and may participate in the cell-type specificity of the ligand effect.

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Identification and Estrogen Induction of Two Estrogen Receptors (ER) Messenger Ribonucleic Acids in the Rainbow Trout Liver: Sequence Homology with other ERs

Cited by 170 publications

References 29 publications

In vivo estrogen induction of hepatic estrogen receptor mRNA and correlation with vitellogenin mRNA in rainbow trout

In vivo estrogen induction of hepatic estrogen receptor mRNA and correlation with vitellogenin mRNA in rainbow trout

Absence of direct regulation of prolactin cells by estradiol-17β in rainbow trout (Oncorhynchus mykiss)

The Nuclear Orphan Receptors COUP-TF and ARP-1 Positively Regulate the Trout Estrogen Receptor Gene through Enhancing Autoregulation

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