Identification and Estimation of Total Tannins from Citrus limon l. Burm. F. (lemon) and its endophytes

Abstract

Citrus limon (Lemon) is a member of the Rutaceae family and it is also known as Kagdi Limbu or Lime. The purpose of the study was to determine the total tannin content of Citrus limon plant and endophytes isolated from the leaves, steam, root, seed, and peel. By using Bromocresol green approach and HPLC with Gallic acid and tannic acid as standards, the total tannin content (TTC) was determined spectrophotometrically. Tannins present in excess amount. Leaf extricates had the highest tannin content of the various sections studied. TLC, spectrophotometric, FTIR, HPLC, and GCMS tests were used to determine the total tannins content of different Endophytes isolated from whole Citrus limon plant vs extract of Citrus limon plant. Intriguingly, a close examination of all out tannins in Endophytes was completed, which can be used.