Identification and enhancement of antioxidant P1-peptide isolated from<i>Ganoderma lucidum</i>hydrolysate

Krobthong, Sucheewin; Yingchutrakul, Yodying

doi:10.1080/08905436.2020.1844228

Cited by 15 publications

(11 citation statements)

References 24 publications

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“…For example, 28785 documents are related to Ganoderma when searched using SciFinder till August 29, 2021. The taste and functional components of the Ganoderma fungus lead to its applications as food additives, raw materials, dietary supplements and remedies, even in Western countries ( Leskosek-Cukalovic et al, 2010 ; Veljović et al, 2019 ; Krobthonga and Yingchutrakul, 2020 ). Polysaccharides and triterpenoids have long been considered the major chemical compositional elements of Ganoderma , whereas the other chemical composition remains largely unknown.…”

Section: Introductionmentioning

confidence: 99%

Meroterpenoids From Ganoderma lucidum Mushrooms and Their Biological Roles in Insulin Resistance and Triple-Negative Breast Cancer

et al. 2021

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Ganoderma fungi as popular raw materials of numerous functional foods have been extensively investigated. In this study, five pairs of meroterpenoid enantiomers beyond well-known triterpenoids and polysaccharides, dayaolingzhiols I−M (1–5), were characterized from Ganoderma lucidum. Their structures were identified using spectroscopic and computational methods. Structurally, compound 1 features a novel dioxabicyclo[2.2.2]octan-3-one motif in the side chain. Ethnoknowledge-derived biological evaluation found that (+)-5 could activate Akt and AMPK phosphorylation in insulin-stimulated C2C12 cells, and (+)-5 could activate glucose uptake dose dependently in C2C12 cells. Furthermore, we found that (+)-1 (+)-4, and (–)-4 could significantly inhibit cell migration of the MDA-MB-231 cell line, of which (+)-4 showed significant inhibitory effects against cell migration of the MDA-MB-231 cell line in a dose-dependent manner. These findings revealed the meroterpenoidal composition of G. lucidum and its roles in the prevention of chronic diseases such as diabetes mellitus and triple-negative breast cancer.

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Section: Introductionmentioning

confidence: 99%

Meroterpenoids From Ganoderma lucidum Mushrooms and Their Biological Roles in Insulin Resistance and Triple-Negative Breast Cancer

et al. 2021

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“…4.1 | Pharmacological/biological assays G. lucidum hydrolysate was prepared as described previously. [7] The tyrosinase-inhibitory peptide was analyzed by Impact II LC-MS/MS (Bruker Daltonics Ltd.). Briefly, the peptides were injected into the UltiMate 3000 LC System (Thermo Fisher Scientific), which consisted of a trapping column (PepMap100, C 18 , 300 μm × 5 mm, i.d.)…”

Section: Methodsmentioning

confidence: 99%

“…In general, the novel functional peptides in food materials are discovered using proteases for hydrolyzing the proteins. [6,7] Such novel functional peptides with health-promoting effects can be identified, purified, modified, and developed into functional ingredients in commercial food products.…”

Section: Introductionmentioning

confidence: 99%

The C‐terminally shortened analogs of a hexapeptide derived from Lingzhi hydrolysate with enhanced tyrosinase‐inhibitory activity

Krobthong

Yingchutrakul

Samutrtai

et al. 2021

Archiv der Pharmazie

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Ganoderma lucidum or Lingzhi (Chinese) is a medicinal fungus widely used in traditional medicine as a health supplement. This study was conducted to identify an approach to enhance the anti-tyrosinase activity of a peptide from G. lucidum by chemical modification of its C-terminus. The original peptide was obtained from protease-digested Lingzhi proteins, followed by ultrafiltration (molecular weight cut-off 3 kDa) and C18 solid-phase extraction. The hexapeptide (NH 2 -VLTCGF-COOH) possessing the anti-tyrosinase activity was identified by liquid chromatography-tandem mass spectrometry (LC-MS/MS). This hexapeptide was subjected to shortening to enhance the anti-tyrosinase activity. Both the original peptide and the shortened peptides were synthesized by solid-phase peptide synthesis. The purity and mass of the synthetic peptide and the modified peptide were evaluated by high-performance liquid chromatography and LC-MS, respectively. Comparison of the tyrosinase activities showed that the modified peptide demonstrated more than 23.27 ± 1.07% activity, which was better than that of the hexapeptide. The structure-related biological activity was explained by molecular docking, wherein the VLT-tyrosinase complex showed two interaction forces: Asn260 and Gly281 through H-bonding and Glu256 through electrostatic interaction. This information could help toward gaining further understanding of the correlation between the anti-tyrosinase activity and the molecular structure of the modified hexapeptide and support its potential use as a safe cosmetic ingredient with tyrosinase-suppressing ability.

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“…Several antioxidant compounds, such as gallic acid, ascorbic acid, or peptides enhanced cell resistance to ROS and prevent skin inflammation [8,9]. Although there are synthetic antioxidant compounds, such as butylated hydroxyanisole, tertiary butylhydroquinone, and butylated hydroxytoluene, the adverse effects of these compounds limit their use in cosmeceutical products [10,11].…”

Section: Introductionmentioning

confidence: 99%

Cosmeceutical Potentials of Grammatophyllum speciosum Extracts: Anti-Inflammations and Anti-Collagenase Activities with Phytochemical Profile Analysis Using an Untargeted Metabolomics Approach

Yingchutrakul

Sittisaree²,

Mahatnirunkul

et al. 2021

Cosmetics

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Grammatophyllum speciosum is the largest orchid species and a well-known traditional medicinal plant. Due to skin aging, natural products that inhibit this process can attract the attention of consumers and scientists because radical-scavenging activity, collagenase inhibition, and inflammatory suppression are valuable in dermatological applications. This study investigated the phytochemicals in G. speciosum leaves extracts that have cosmeceutical potentials, including radical-scavenging, anticollagenase, and anti-inflammatory abilities. G. speciosum leaves were extracted using water-based extraction methods. High-resolution mass spectrometry was used to identify the phytochemicals in the extracts. Fibroblast and keratinocyte cell cytotoxicity was determined. Antioxidant abilities were measured using DPPH and ABTS assays. The effect of the extracts on nitric oxide (NO) in macrophage cells was investigated. ELISA of the collagenase enzyme was determined. A total of 721 annotated metabolites were identified in the extracts. Vitexin and orientin were the most abundant metabolites. Cell viability was >80% in both cell lines when the extract concentration was <1 mg/mL. The IC50 values for DPPH and ABTS were 56 and 117 μg/mL, respectively. Furthermore, the extracts revealed that NO and collagenase activity were suppressed by 42% and 23%, respectively. The extracts can suppress ROS, inflammatory, and collagenase activities without causing fibroblast and keratinocyte cell death. Thus, this study provides information on metabolites in G. speciosum leaves, which is promising as cosmeceuticals or pharmaceuticals with anti-inflammatory and anti-collagenase activities.

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Identification and enhancement of antioxidant P1-peptide isolated fromGanoderma lucidumhydrolysate

Cited by 15 publications

References 24 publications

Meroterpenoids From Ganoderma lucidum Mushrooms and Their Biological Roles in Insulin Resistance and Triple-Negative Breast Cancer

Meroterpenoids From Ganoderma lucidum Mushrooms and Their Biological Roles in Insulin Resistance and Triple-Negative Breast Cancer

The C‐terminally shortened analogs of a hexapeptide derived from Lingzhi hydrolysate with enhanced tyrosinase‐inhibitory activity

Cosmeceutical Potentials of Grammatophyllum speciosum Extracts: Anti-Inflammations and Anti-Collagenase Activities with Phytochemical Profile Analysis Using an Untargeted Metabolomics Approach

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