Identification and elimination of metabolic bottlenecks in the quinone modification pathway for enhanced coenzyme Q10 production in Rhodobacter sphaeroides

Lu, Wenqiang; Ye, Lidan; Lv, Xiaomei; Xie, Wenping; Gu, Jiali; Chen, Zhaofeng; Zhu, Yujun; Li, Aipeng; Yu, Hongwei

doi:10.1016/j.ymben.2015.03.012

Cited by 48 publications

(53 citation statements)

References 41 publications

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“…More complex structures are usually found in living organisms, and these have heterocyclic motifs or fused rings, such as naphthohydroquinones or anthraquinones . Quinones have diverse biosynthetic pathways, but the best known in higher plants involve the acetate–malonate and shikimic acid routes . Plant quinones are usually employed in energy and hydrogen transport, such as coenzyme Q 10 and plastoquinone, to provide essential nutrients in the case of vitamin K or to serve as cocatalysts like pyrroloquinoline quinone …”

Section: Chemistrymentioning

confidence: 99%

Recent advances in allelopathy for weed control: from knowledge to applications

Macías

Mejías

Molinillo

2019

Pest Management Science

199

131

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Allelopathy is the biological phenomenon of chemical interactions between living organisms in the ecosystem, and must be taken into account in addressing pest and weed problems in future sustainable agriculture. Allelopathy is a multidisciplinary science, but in some cases, aspects of its chemistry are overlooked, despite the need for a deep knowledge of the chemical structural characteristics of allelochemicals to facilitate the design of new herbicides. This review is focused on the most important advances in allelopathy, paying particular attention to the design and development of phenolic compounds, terpenoids and alkaloids as herbicides. The isolation of allelochemicals is mainly addressed, but other aspects such as the analysis and activities of derivatives or analogs are also covered. Furthermore, the use of allelopathy in the fight against parasitic plants is included. The past 12 years have been a prolific period for publications on allelopathy. This critical review discusses future research areas in this field and the state of the art is analyzed from the chemist's perspective. © 2019 Society of Chemical Industry

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Section: Chemistrymentioning

confidence: 99%

Recent advances in allelopathy for weed control: from knowledge to applications

Macías

Mejías

Molinillo

2019

Pest Management Science

199

131

View full text Add to dashboard Cite

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“…Several native producers of CoQ 10 have been identified or optimized as candidates for CoQ 10 production, including S. pombe , S. johnsonii , Rhodobacter sphaeroides and Agrobacterium tumefaciens [78, 83, 87, 88]. Several other organisms, including Pseudomonas , Paracoccus bacteria, Candida and Saitoella yeasts also produce CoQ 10 natively but have not been sufficiently characterized as producing hosts, and many require the inclusion of expensive constituents in the growth media for proper function.…”

Section: Introductionmentioning

confidence: 99%

“…Promoter-based balancing of metabolic flux increased the production to 7.16–8.7 mg/g DCW [60, 98], and a recent study reported production as high as 12.96 mg/g DCW [87] (Table 1). However, other efforts to increase MEP pathway flux did not translate well into increased CoQ 10 production, probably due to an accumulation of toxic intermediates [99].…”

Section: Introductionmentioning

confidence: 99%

“…Neither A. tumefaciens nor R. sphaeroides produce sufficient quantities of CoQ 10 to meet current market demands, and this has led to higher prices of CoQ 10 [38]. Furthermore, rather than optimizing the hosts, recent efforts in the field have been to develop toolkit pieces, such as promoter-regulated vectors [98, 99], or to determine ways to select for particular strains after mutagenesis [105]; only a few studies have attempted to harness metabolic engineering (to increase gene expression) or protein engineering [83, 87]. Other efforts garnered toward a more immediate solution have had to rely on the addition of precursors to increase yield, and this comes at a higher cost and therefore remains less feasible [106, 107].…”

Section: Introductionmentioning

confidence: 99%

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Cellular factories for coenzyme Q10 production

et al. 2017

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Coenzyme Q10 (CoQ10), a benzoquinone present in most organisms, plays an important role in the electron-transport chain, and its deficiency is associated with various neuropathies and muscular disorders. CoQ10 is the only lipid-soluble antioxidant found in humans, and for this, it is gaining popularity in the cosmetic and healthcare industries. To meet the growing demand for CoQ10, there has been considerable interest in ways to enhance its production, the most effective of which remains microbial fermentation. Previous attempts to increase CoQ10 production to an industrial scale have thus far conformed to the strategies used in typical metabolic engineering endeavors. However, the emergence of new tools in the expanding field of synthetic biology has provided a suite of possibilities that extend beyond the traditional modes of metabolic engineering. In this review, we cover the various strategies currently undertaken to upscale CoQ10 production, and discuss some of the potential novel areas for future research.

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“…Currently, the rate‐limiting steps are screened through experimental approaches. Lu et al identified a rate‐limiting step in coenzyme Q10 biosynthesis by overexpressing individual enzymes involved in the quinone modification pathway. Pitera et al identified a rate‐limiting step in isoprenoid production by exogenously supplementing a precursor in the culture medium.…”

Section: Introductionmentioning

confidence: 99%

Prediction of Rate‐Limiting Reactions for Growth‐Associated Production Using a Constraint‐Based Approach

Tokuyama

Toya

Shimizu

2019

Biotechnology Journal

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Identification of a rate‐limiting step in pathways is a key challenge in metabolic engineering. Although the prediction of rate‐limiting steps using a kinetic model is a powerful approach, there are several technical hurdles for developing a kinetic model. In this study, an in silico screening algorithm of key enzyme for metabolic engineering is developed to identify the possible rate‐limiting reactions for the growth‐coupled target production using a stoichiometric model without any experimental data and kinetic parameters. In this method, for each reaction, an upper‐bound flux constraint is imposed and the target production is predicted by linear programming. When the constraint decreases the target production at the optimal growth state, the reaction is thought to be a possible rate‐limiting step. For validation, this method is applied to the production of succinate or 1,4‐butanediol (1,4‐BDO) in Escherichia coli, in which the experimental engineering for eliminating rate‐limiting steps has been previously reported. In succinate production from glycerol, nine reactions including phosphoenolpyruvate carboxylase are predicted as the rate‐limiting steps. In 1,4‐BDO production from glucose, eight reactions including pyruvate dehydrogenase are predicted as the rate‐limiting steps. These predictions include experimentally identified rate‐limiting steps, which would contribute to metabolic engineering as a practical tool for screening candidates of rate‐limiting reactions.

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Identification and elimination of metabolic bottlenecks in the quinone modification pathway for enhanced coenzyme Q10 production in Rhodobacter sphaeroides

Cited by 48 publications

References 41 publications

Recent advances in allelopathy for weed control: from knowledge to applications

Recent advances in allelopathy for weed control: from knowledge to applications

Cellular factories for coenzyme Q10 production

Prediction of Rate‐Limiting Reactions for Growth‐Associated Production Using a Constraint‐Based Approach

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